60nm Carboxyl Red-Fluo Microspheres CFR060-10 Overview

CFR060-10 60nm Carboxyl Red-Fluo Microspheres are high-performance fluorescent polymer microspheres designed for lateral flow immunoassay (LFIA), fluorescent immunoassay development, antibody conjugation, and diagnostic reagent manufacturing.

With a controlled particle size of 60nm, red fluorescent signal, carboxyl-functionalized surface, and 0.5% solids concentration, CFR060-10 provides a reliable labeling platform for biotechnology companies, diagnostic reagent developers, and research institutions.

The surface carboxyl groups enable covalent coupling with antibodies, antigens, proteins, peptides, and other biomolecules through EDC/NHS activation chemistry, making them suitable for developing fluorescent conjugates with improved stability and reproducibility.

CFR060-10 supports applications from early-stage assay research to pilot-scale validation and bulk production of fluorescent lateral flow reagents.

Product Specifications of CFR060-10 Red Fluorescent Microspheres

Key Features of 60nm Carboxyl Red-Fluo Microspheres

60nm Particle Size for Fluorescent Immunoassay Development

CFR060-10 features a nanoscale particle diameter of approximately 60nm.

Compared with larger fluorescent latex particles, 60nm microspheres provide:

• High particle number concentration

• Large surface area for biomolecule interaction

• Good dispersion stability

• Efficient migration characteristics

• Flexible optimization for fluorescent lateral flow systems

The 60nm size provides a balance between nanoscale mobility and fluorescent signal performance, making it suitable for researchers developing high-sensitivity immunoassays.

Red Fluorescent Signal for Quantitative Detection

CFR060-10 contains red fluorescent dye incorporated into the polymer matrix.

The fluorescent signal can be detected using compatible fluorescence readers, supporting:

• Quantitative lateral flow assays

• Digital immunochromatography

• Instrument-based detection

• Multiplex assay development

• Research diagnostic platforms

Compared with traditional visible labels, fluorescence-based detection allows objective signal measurement and reduces interpretation differences caused by visual evaluation.

Carboxyl Functional Surface for Antibody Coupling

The surface of CFR060-10 contains carboxyl groups (-COOH), providing reactive sites for biomolecule immobilization.

Using EDC or EDC/NHS chemistry, the microspheres can be conjugated with:

• Monoclonal antibodies

• Polyclonal antibodies

• Antigens

• Recombinant proteins

• Peptides

• Enzymes

• Other amine-containing biomolecules

The covalent coupling method provides stable attachment and supports consistent fluorescent conjugate preparation.

Uniform Particle Size and Batch Consistency

Particle size consistency is critical for fluorescent lateral flow assay performance.

CFR060-10 is manufactured with controlled particle distribution to support:

• Reproducible conjugation

• Stable suspension

• Consistent membrane migration

• Reliable assay development

• Batch-to-batch quality

Applications of CFR060-10 in Lateral Flow Immunoassays

Fluorescent Lateral Flow Immunoassay (FLIA)

CFR060-10 can be used as a fluorescent reporter material for lateral flow assay development.

Typical applications include:

• Infectious disease detection research

• Biomarker analysis

• Food safety testing

• Veterinary diagnostics

• Environmental detection

• Pharmaceutical research

The fluorescent signal enables integration with fluorescence readers for quantitative analysis.

Antibody and Protein Labeling

Carboxyl fluorescent microspheres provide a convenient platform for antibody and protein conjugation.

Common biomolecules include:

• Detection antibodies

• Capture antibodies

• Antigens

• Binding proteins

• Peptides

• Nucleic acid probes

Researchers can optimize coupling density according to assay requirements.

Quantitative Immunochromatography Development

Fluorescent LFIA platforms are increasingly used for quantitative detection.

CFR060-10 supports development of systems requiring:

• Numerical fluorescence output

• Reader-based analysis

• Improved detection evaluation

• Digital result interpretation

Diagnostic Reagent Manufacturing

CFR060-10 is suitable for companies developing commercial immunochromatography products.

Applications include:

• Raw material screening

• Conjugate optimization

• Prototype development

• Pilot manufacturing

• Process transfer

• Bulk reagent production

How Do 60nm Carboxyl Red-Fluo Microspheres Work in LFIA?

In a typical fluorescent lateral flow assay:

1. CFR060-10 microspheres are conjugated with detection antibodies.

2. The sample is added onto the test strip.

3. Target molecules bind with fluorescent antibody conjugates.

4. The complex migrates through the nitrocellulose membrane.

5. The target complex is captured at the test line.

6. Fluorescence intensity is measured by a fluorescence reader.

7. The signal is converted into qualitative or quantitative results.

The final assay performance depends on:

• Antibody affinity

• Coupling efficiency

• Particle concentration

• Membrane selection

• Buffer formulation

• Reader sensitivity

Antibody Coupling of CFR060-10 Carboxyl Microspheres

Carboxyl Activation

The microsphere surface carboxyl groups are activated using EDC or EDC/NHS chemistry.

Activation efficiency depends on:

• pH condition

• Reaction time

• EDC concentration

• Microsphere concentration

Protein Conjugation

Activated microspheres react with primary amino groups of antibodies or proteins.

Important optimization parameters include:

• Protein concentration

• Particle-to-protein ratio

• Reaction temperature

• Incubation time

• Buffer system

Blocking and Purification

After coupling, remaining active groups are blocked and unbound proteins are removed.

Purification methods may include:

• Centrifugation

• Filtration

• Dialysis

• Chromatography

Conjugate Performance Evaluation

Recommended evaluation parameters:

• Particle size distribution

• Fluorescence intensity

• Coupling efficiency

• Dispersion stability

• LFIA strip performance

Advantages of CFR060-10 Compared with Traditional Labels

Quality Control for Research and Bulk Production

For diagnostic reagent manufacturers, raw material consistency directly affects final product performance.

CFR060-10 quality control includes:

Particle Size Analysis

Ensures consistent migration behavior and conjugation performance.

Fluorescence Performance

Evaluates fluorescent intensity and optical stability.

Surface Functional Group Testing

Confirms available carboxyl groups for biomolecule coupling.

Solid Content Control

Ensures consistent formulation during conjugate preparation.

Batch Consistency

Supports reliable transition from laboratory development to production.

Customization Options for CFR060-10

SHBC provides customized fluorescent microsphere solutions according to customer assay requirements.

Particle Size Customization

Available sizes can be optimized for:

• Membrane migration

• Detection sensitivity

• Reader compatibility

Fluorescence Customization

Options include:

• Different fluorescent colors

• Customized emission characteristics

• Reader matching

Surface Chemistry Customization

Available functional groups:

• Carboxyl

• Amino

• Streptavidin

• Other customized surfaces

Concentration Customization

Solid content can be adjusted according to:

• Coupling process

• Shipping requirements

• Production workflow

OEM and Bulk Supply

SHBC supports:

• Research samples

• Pilot batches

• Bulk manufacturing

• Customized packaging

• Long-term supply cooperation

Frequently Asked Questions About CFR060-10

What are 60nm Carboxyl Red-Fluo Microspheres?

CFR060-10 are 60nm fluorescent polymer microspheres with carboxyl surface groups designed for LFIA, immunoassay development and antibody conjugation.

Can CFR060-10 be used for lateral flow assays?

Yes. CFR060-10 is developed for fluorescent lateral flow assay research and reagent production applications.

How are antibodies attached to CFR060-10?

Antibodies are commonly coupled through EDC/NHS chemistry, forming stable amide bonds between antibody amino groups and microsphere carboxyl groups.

Why choose 60nm fluorescent microspheres?

60nm microspheres provide a balance between particle mobility, surface area, fluorescence performance and conjugation flexibility.

What is the recommended application concentration?

The optimal concentration depends on antibody loading, membrane type, reader sensitivity and assay design. Optimization is recommended during development.

Can CFR060-10 be supplied in bulk?

Yes. SHBC provides laboratory samples, pilot-scale quantities and bulk production supply.

Can particle specifications be customized?

Yes. Particle size, fluorescence properties, surface chemistry, solids concentration and packaging can be customized.

Why Choose SHBC CFR060-10 Carboxyl Red-Fluo Microspheres?

SHBC specializes in fluorescent microsphere materials for immunodiagnostic research and reagent manufacturing.

CFR060-10 provides:

• Controlled 60nm particle size

• Red fluorescent labeling

• Carboxyl surface chemistry

• Reliable antibody coupling capability

• LFIA development support

• Research-to-production supply

• OEM and customization services

Contact SHBC for technical specifications, samples, and bulk quotation for 60nm Carboxyl Red-Fluo Microspheres CFR060-10.