7um Carboxyl Blue-Fluo Microspheres CFB7UM-10

7um Carboxyl Blue-Fluo Microspheres CFB7UM-10

SHBC CFB7UM-10 are 7um blue fluorescent microspheres with reactive carboxyl groups on the particle surface.

With excitation at 366nm and emission at 460nm, the microspheres provide a clear blue fluorescent signal for fluorescence microscopy, UV-compatible flow cytometry, cell tracking, particle-based assays and biomolecule conjugation.

Product Specifications

Key Product Advantages

• Blue fluorescence at 460nm

• Compatible with suitable 366nm UV excitation

• Reactive carboxyl-functionalized surface

• Controlled 7um particle size

• Strong fluorescence per particle

• Easy microscopic observation

• Large surface for biomolecule immobilization

• Suitable for research and batch production

Why Choose 7um Blue Fluorescent Microspheres?

The 7um particle size provides strong particle-level fluorescence and clear optical identification.

CFB7UM-10 offers:

• Easy particle counting

• Clear microscopic localization

• Strong single-particle fluorescence

• Large biomolecule-loading surface

• Convenient flow cytometry discrimination

• Good suitability for cell–particle interaction studies

• Easy separation from smaller particles

Carboxyl Surface for Biomolecule Coupling

CFB7UM-10 contains reactive carboxyl groups for covalent conjugation with primary amine-containing biomolecules.

Compatible ligands include:

• Antibodies

• Antigens

• Proteins

• Peptides

• Enzymes

• Oligonucleotides

• Other amine-containing molecules

The surface carboxyl groups can be activated using EDC/NHS chemistry to form stable amide bonds with target biomolecules.

Main Applications

Fluorescence Microscopy

The 7um particle size and blue fluorescent signal support easy observation using compatible fluorescence microscopes.

Typical applications include:

• Particle localization

• Fluorescence imaging

• Particle counting

• Surface-binding studies

• Material distribution analysis

• Particle movement tracking

UV-Compatible Flow Cytometry

CFB7UM-10 can be evaluated for flow cytometry systems equipped with a suitable UV excitation source and blue fluorescence detector.

Potential uses include:

• Fluorescent particle counting

• Particle population analysis

• Surface-binding assays

• Fluorescence channel evaluation

• Method development

• Multiplex bead research

Cell Tracking and Phagocytosis Research

The microspheres can be evaluated for studying interactions between cells and fluorescent particles.

Potential applications include:

• Cell–particle binding

• Particle uptake studies

• Phagocytosis research

• Immune cell interaction

• Particle adhesion

• Intracellular localization

Particle concentration and incubation conditions should be optimized for each cell model.

Particle-Based Immunoassays

Antibodies, antigens or proteins can be immobilized on the carboxyl surface to prepare fluorescent capture particles.

Potential assay formats include:

• Fluorescent binding assays

• Sandwich immunoassays

• Competitive assays

• Antibody detection

• Antigen detection

• Fluorescent agglutination research

Multiplex Suspension Assays

CFB7UM-10 can be combined with microspheres of different sizes, fluorescence colors or surface ligands to create distinguishable particle populations.

Potential applications include:

• Multiplex immunoassays

• Suspension arrays

• Liquid-phase biochips

• Protein detection panels

• Nucleic acid hybridization research

Biosensors and Microfluidics

The carboxyl surface supports biological ligand immobilization for biosensors, biochips and microfluidic systems.

The blue fluorescent signal helps visualize particle capture, movement and distribution.

Particle Tracking and Fluid Tracing

CFB7UM-10 can be used for:

• Fluid-flow visualization

• Filtration studies

• Microfluidic tracking

• Particle transport research

• Porous-media analysis

• Material distribution testing

Recommended Coupling Workflow

1. Mix the microsphere suspension thoroughly.

2. Wash with a suitable activation buffer.

3. Activate the surface carboxyl groups using EDC and NHS.

4. Add the antibody, protein or target ligand.

5. Incubate under optimized reaction conditions.

6. Block the remaining active sites.

7. Wash the conjugated microspheres.

8. Resuspend in a suitable storage buffer.

9. Evaluate coupling efficiency and fluorescence performance.

Coupling pH, reagent concentration, reaction time and blocking conditions should be optimized for each biomolecule.

Handling Recommendations

• Mix gently before sampling.

• Protect the product from strong light.

• Avoid prolonged high-power ultrasonication.

• Do not allow the microspheres to dry.

• Avoid repeated freezing and thawing.

• Use clean, low-binding consumables.

• Confirm buffer compatibility before scale-up.

• Follow the product label for storage conditions.

Application Notes

For flow cytometry, confirm that the instrument has a compatible UV excitation source and blue emission channel.

For fluorescence microscopy, use optical filters suitable for 366nm excitation and 460nm emission.

The 7um particle size is larger than conventional fluorescent lateral flow reporters. Membrane migration must be fully validated before use in lateral flow assays.

Customization and Bulk Supply

SHBC supports fluorescent microsphere projects from laboratory evaluation to batch manufacturing.

Customization options include:

• Different particle sizes

• Different fluorescence wavelengths

• Fluorescence intensity adjustment

• Surface carboxyl density adjustment

• Alternative functional groups

• Solids-content adjustment

• Custom suspension buffers

• Custom packaging

• OEM and ODM manufacturing

Why Choose SHBC?

Shanghai SanYu Biotechnology Co., Ltd. manufactures functional microspheres and magnetic beads for fluorescence detection, immunoassays, particle analysis and biological research.

SHBC provides:

• Controlled particle-size production

• Stable fluorescence performance

• Functional surface modification

• Reliable batch consistency

• Biomolecule coupling support

• Research samples

• Flexible packaging

• Pilot-scale and bulk production

Frequently Asked Questions

What is CFB7UM-10?

CFB7UM-10 is a 7um carboxyl-functionalized blue fluorescent microsphere suspension with 1% solids content.

What are the excitation and emission wavelengths?

The excitation wavelength is 366nm, and the emission wavelength is 460nm.

Can CFB7UM-10 be coupled with antibodies?

Yes. The surface carboxyl groups can be activated using EDC/NHS chemistry for covalent antibody or protein conjugation.

Is CFB7UM-10 suitable for flow cytometry?

It can be used with flow cytometers equipped with a compatible UV excitation source, blue fluorescence detector and fluidics suitable for 7um particles.

Can it be used for fluorescence microscopy?

Yes. The 7um particle size provides easy visualization, counting, localization and particle tracking.

Can it be used for cell uptake studies?

CFB7UM-10 can be evaluated for phagocytosis, cell uptake and cell–particle interaction research.

Is it suitable for multiplex assays?

Yes. Different particle sizes, fluorescence colors or surface ligands can be used to create distinguishable bead populations.

Is it suitable for lateral flow assays?

The 7um particle size is larger than conventional LFIA reporter particles. Membrane migration and assay compatibility must be validated.

Can SHBC provide samples and bulk quantities?

Yes. SHBC supports research samples, pilot-scale evaluation and bulk manufacturing.

Can the product be customized?

Yes. Particle size, fluorescence wavelength, surface functional group, solids content, suspension buffer and packaging can be customized.

Ordering Information

Product Name: 7um Carboxyl Blue-Fluo Microspheres
Catalog Number: CFB7UM-10
Particle Size: 7um
Solids Content: 1%
Excitation Wavelength: 366nm
Emission Wavelength: 460nm
Surface Group: Carboxyl, COOH
Brand: SHBC
Manufacturer: Shanghai SanYu Biotechnology Co., Ltd.

Contact SHBC for samples, technical documents, customized specifications and bulk production quotations.

For research and manufacturing use. Performance in the final application should be validated by the customer.