7um Carboxyl Green-Fluo Microspheres CFG7UM-10 1%

7um Carboxyl Green-Fluo Microspheres CFG7UM-10

SHBC CFG7UM-10 Carboxyl Green-Fluo Microspheres are uniform 7um fluorescent polymer particles with reactive carboxyl groups on the surface.

With excitation at 470/488nm and emission at 525nm, these microspheres provide a bright green fluorescent signal for flow cytometry, fluorescence microscopy, cell tracking, immunoassay development and biomolecule conjugation.

Product Specifications

Key Product Advantages

• Bright green fluorescence at 525nm

• Compatible with 470nm and 488nm excitation

• Reactive carboxyl surface

• Uniform 7um particle size

• High fluorescence signal per particle

• Easy detection by microscopy

• Large surface area for biomolecule loading

• Suitable for flow cytometry and cell research

• Available for research and batch production

Why Choose 7um Green Fluorescent Microspheres?

The 7um particle size provides high fluorescence per particle and clear optical identification.

Compared with smaller fluorescent microspheres, CFG7UM-10 offers:

• Easier visualization under fluorescence microscopy

• Stronger signal from individual particles

• More surface area for antibody or protein immobilization

• Clear particle discrimination in flow-based systems

• Convenient particle counting and tracking

• Good suitability for cell–particle interaction studies

Carboxyl Surface for Covalent Coupling

CFG7UM-10 contains surface carboxyl groups that can be activated for covalent conjugation with primary amine-containing biomolecules.

Compatible ligands include:

• Antibodies

• Antigens

• Proteins

• Peptides

• Enzymes

• Oligonucleotides

• Other amino-containing molecules

EDC/NHS chemistry can be used to form stable amide bonds between the microspheres and target biomolecules.

Main Applications

Flow Cytometry

The 7um particle size is readily distinguished by common flow cytometry systems.

CFG7UM-10 can be evaluated for:

• Fluorescent particle analysis

• Flow cytometer performance testing

• Fluorescence channel evaluation

• Particle counting

• Binding assay development

• Microsphere population discrimination

Fluorescence Microscopy

The micrometer-scale size and bright green signal make the particles easy to observe using a compatible fluorescence microscope.

Typical uses include:

• Particle localization

• Fluorescence imaging

• Particle tracking

• Surface binding studies

• Material distribution analysis

• Microfluidic visualization

Cell Labeling and Tracking

CFG7UM-10 can be used in research involving interactions between cells and fluorescent particles.

Potential applications include:

• Cell uptake studies

• Cell–particle binding

• Phagocytosis research

• Intracellular transport studies

• Immune cell research

• Particle localization around cells

Fluorescence Immunoassays

Antibodies, antigens or proteins can be immobilized on the carboxyl surface to prepare fluorescent capture or detection particles.

The microspheres can be evaluated for:

• Particle-based immunoassays

• Sandwich binding assays

• Competitive assays

• Antibody detection

• Antigen detection

• Fluorescent agglutination research

Multiplex Suspension Assays

Different particle sizes, fluorescence levels or surface ligands can be used to create distinguishable microsphere populations.

CFG7UM-10 can support the development of:

• Suspension arrays

• Multiplex immunoassays

• Liquid-phase biochips

• Protein detection panels

• Nucleic acid hybridization research

Biosensors and Microfluidics

The carboxyl-functionalized surface supports biological ligand immobilization for biosensors, biochips and microfluidic platforms.

The clear fluorescent signal helps visualize particle movement, capture and distribution.

Instrument and Method Development

Uniform fluorescent particles can be evaluated for optical alignment, detector response and fluorescence method development.

CFG7UM-10 is supplied as a research material and should not be described as a certified calibration standard unless separately certified.

Recommended Coupling Workflow

1. Mix the microsphere suspension thoroughly.

2. Wash the microspheres with a suitable activation buffer.

3. Activate the carboxyl groups using EDC and NHS.

4. Add the antibody, protein or target ligand.

5. Incubate under optimized reaction conditions.

6. Block the remaining active sites.

7. Wash the conjugated microspheres.

8. Resuspend in a suitable storage buffer.

9. Evaluate coupling efficiency and fluorescence performance.

Reaction pH, reagent concentration, coupling time and blocking conditions should be optimized for each biomolecule.

Recommended Handling

• Mix gently before use.

• Protect the suspension from strong light.

• Avoid prolonged high-power ultrasonication.

• Do not allow the microspheres to dry.

• Avoid repeated freezing and thawing.

• Use clean, low-binding laboratory consumables.

• Confirm buffer compatibility before scale-up.

• Follow the product label for storage requirements.

Application Notes

Because 7um microspheres are larger than nanoparticles commonly used in lateral flow assays, membrane migration should be carefully evaluated.

For flow cytometry and microscopy, users should optimize:

• Particle concentration

• Detector voltage

• Fluorescence filters

• Flow rate

• Sample dispersion

• Gating strategy

For cell experiments, particle concentration and incubation time should be optimized for the selected cell type.

Customization and Bulk Supply

SHBC supports fluorescent microsphere projects from laboratory evaluation to batch manufacturing.

Customization options include:

• Different particle sizes

• Different fluorescence wavelengths

• Fluorescence intensity adjustment

• Surface carboxyl density adjustment

• Alternative functional groups

• Solids-content adjustment

• Custom suspension buffers

• Custom packaging

• OEM and ODM production

Why Choose SHBC?

Shanghai SanYu Biotechnology Co., Ltd. manufactures functional microspheres and magnetic beads for fluorescence detection, immunoassays, bioseparation and biological research.

SHBC provides:

• Controlled particle-size production

• Stable fluorescence performance

• Functional surface modification

• Reliable batch-to-batch consistency

• Biomolecule coupling support

• Research samples

• Flexible packaging

• Pilot-scale and bulk production

Frequently Asked Questions

What is CFG7UM-10?

CFG7UM-10 is a 7um carboxyl-functionalized green fluorescent microsphere suspension with 1% solids content.

What are the excitation and emission wavelengths?

The recommended excitation wavelengths are 470nm and 488nm. The emission wavelength is 525nm.

Is CFG7UM-10 compatible with a 488nm laser?

Yes. It can be excited using a compatible 488nm laser and detected through a suitable green fluorescence channel.

Can the microspheres be coupled with antibodies?

Yes. The surface carboxyl groups can be activated using EDC/NHS chemistry for covalent antibody or protein conjugation.

Is CFG7UM-10 suitable for flow cytometry?

Yes. Its 7um particle size and green fluorescence make it suitable for flow cytometry research, particle counting and binding assay development.

Can it be used for fluorescence microscopy?

Yes. The large particle size and bright green fluorescence support easy visualization, localization and particle tracking.

Can it be used for cell uptake studies?

Yes. CFG7UM-10 can be evaluated for phagocytosis, cell uptake and cell–particle interaction research.

Is it suitable for lateral flow assays?

The 7um particle size is larger than particles commonly used in lateral flow tests. Membrane migration and assay compatibility must be validated before use.

Can SHBC provide samples?

Yes. Samples are available for coupling evaluation, fluorescence testing and application development.

Is bulk production available?

Yes. SHBC supports pilot-scale orders and bulk manufacturing for research institutions, biotechnology companies and reagent manufacturers.

Can the product be customized?

Yes. Particle size, fluorescence wavelength, surface group, solids content, buffer and packaging can be customized.

Ordering Information

Product Name: 7um Carboxyl Green-Fluo Microspheres
Catalog Number: CFG7UM-10
Particle Size: 7um
Solids Content: 1%
Excitation Wavelength: 470/488nm
Emission Wavelength: 525nm
Surface Group: Carboxyl, COOH
Brand: SHBC
Manufacturer: Shanghai SanYu Biotechnology Co., Ltd.

Contact SHBC for samples, technical documents, customized specifications and bulk production quotations.

For research and manufacturing use. Performance in the final application should be validated by the customer.