7um Carboxyl Red-Fluo Microspheres CFR7UM-10

7um Carboxyl Red-Fluo Microspheres CFR7UM-10

SHBC CFR7UM-10 are 7 μm carboxyl-functionalized red fluorescent microspheres developed for flow cytometry, fluorescence microscopy, cell–particle interaction studies and biomolecule immobilization.

The microspheres feature 535 nm excitation, 610 nm emission and 1% solid content. Surface carboxyl groups support covalent coupling with antibodies, antigens, proteins and peptides.

Product Specifications

Batch-specific particle size, size distribution, fluorescence intensity and carboxyl content are available in the Certificate of Analysis.

Red Fluorescence at 535/610nm

CFR7UM-10 produces red fluorescence with excitation at approximately 535 nm and emission centered at approximately 610 nm.

The fluorescence profile is suitable for compatible flow cytometers, fluorescence microscopes, imaging systems and fluorescence readers.

Key Advantages

Uniform 7um Particle Size

Controlled particle sizing supports repeatable fluorescence detection, surface coupling and particle-based analysis.

Strong Signal per Particle

The large 7 μm particle body can carry a high fluorescent signal, supporting clear particle identification.

Reactive Carboxyl Surface

Surface COOH groups provide active sites for covalent immobilization of antibodies, antigens and proteins.

Easy Optical Detection

Micrometer-scale particles are readily detected using compatible flow cytometry and fluorescence imaging systems.

Large Coupling Surface

The 7 μm diameter provides substantial surface area per particle for biomolecule immobilization.

Custom and Bulk Supply

SHBC supports sample evaluation, customized specifications, pilot production and bulk manufacturing.

Main Applications

Flow Cytometry

CFR7UM-10 can be used for particle detection, fluorescence channel evaluation and flow-based assay development.

Fluorescence Microscopy

The red fluorescent signal supports particle localization, distribution and imaging studies.

Phagocytosis Research

The 7 μm particles can be evaluated in macrophage uptake, phagocytosis and immune-cell interaction studies.

Cell–Particle Interaction Studies

Suitable for studying particle adhesion, cellular contact, uptake and surface recognition.

Particle-Based Immunoassays

Antibodies or antigens can be immobilized on the carboxyl surface for suspension immunoassays and agglutination research.

Biomolecule Immobilization

Suitable for coupling proteins, peptides, enzymes and other amino-containing biomolecules.

Microfluidic Research

Can be used in particle transport, channel testing, filtration and fluid-flow studies.

Fluorescence Method Development

Suitable for developing particle detection, imaging, counting and analytical methods.

Biomolecule Coupling

The carboxyl surface can be activated using EDC/NHS chemistry and coupled with primary amine groups on antibodies or proteins.

A typical workflow includes:

1. Wash and redisperse the microspheres.

2. Activate the COOH groups with EDC and NHS.

3. Add the antibody or target protein.

4. Block remaining reactive sites.

5. Wash and resuspend the conjugated particles.

6. Evaluate coupling and fluorescence performance.

Coupling conditions should be optimized for each biomolecule.

Coupling Advantages

CFR7UM-10 provides:

• Reactive surface carboxyl groups

• Large surface area per particle

• Stable covalent immobilization

• Easy recovery by centrifugation

• Strong fluorescence detection

• Flexible ligand modification

• Compatibility with particle-based assays

• Custom conjugation support

Flow Cytometry Considerations

Recommended parameters to optimize include:

• Particle concentration

• Sample flow rate

• Excitation source

• Emission filter

• FSC and SSC settings

• Detector voltage

• Gating strategy

• Doublet discrimination

• Particle aggregation

Use a fluorescence channel configured for emission near 610 nm.

Cell and Phagocytosis Studies

For cellular experiments, evaluate:

• Particle-to-cell ratio

• Incubation time

• Cell type

• Surface ligand density

• Culture conditions

• Washing efficiency

• Internalization controls

• Background fluorescence

Include untreated cells, particle-only controls and appropriate fluorescence controls.

Dispersion and Handling

Before use:

• Mix by gentle inversion or controlled vortexing

• Redisperse settled particles completely

• Avoid excessive foam

• Protect the suspension from strong light

• Prevent the microspheres from drying

• Avoid repeated freeze-thaw cycles

• Confirm buffer compatibility

• Use clean laboratory equipment

Controlled bath sonication may be used to break weak aggregates when required.

Storage Recommendations

Keep the container tightly closed and protected from light.

Do not return unused suspension to the original container. Refer to the product label, Technical Data Sheet and Certificate of Analysis for batch-specific storage conditions.

Custom Carboxyl Fluorescent Microspheres

SHBC can customize fluorescent microspheres according to research and production requirements.

Customization options may include:

• Custom particle size

• Custom fluorescence wavelength

• Different fluorescence colors

• Carboxyl group density

• Solid content

• Dispersion buffer

• Surface modification

• Biomolecule conjugation

• Custom packaging

• OEM and ODM production

Please provide the application, required wavelength, surface ligand and order quantity when requesting customization.

Quality Control and Documents

Available product documents may include:

• Certificate of Analysis

• Technical Data Sheet

• Safety Data Sheet

• Particle size report

• Particle size distribution data

• Fluorescence spectrum

• Carboxyl content data

• Microscopy images

• Batch inspection report

Why Choose SHBC?

Shanghai SanYu Biotechnology Co., Ltd. supplies fluorescent microspheres, colored latex particles, magnetic beads and functional particle materials.

SHBC provides:

• Controlled microsphere manufacturing

• Stable particle size distribution

• Multiple fluorescence wavelengths

• Functional surface modification

• Batch-to-batch consistency

• Sample evaluation support

• Pilot and bulk production

• OEM and ODM services

Frequently Asked Questions

What is CFR7UM-10?

CFR7UM-10 is a 7 μm carboxyl-functionalized red fluorescent microsphere suspension with 1% solid content.

What are its excitation and emission wavelengths?

The excitation wavelength is 535 nm and the emission wavelength is 610 nm.

What is CFR7UM-10 mainly used for?

It is suitable for flow cytometry, fluorescence microscopy, phagocytosis studies, cell–particle interaction and biomolecule coupling.

Can antibodies be coupled to CFR7UM-10?

Yes. Antibodies and proteins containing primary amine groups can be covalently coupled to the carboxyl surface.

Which coupling chemistry is recommended?

EDC/NHS chemistry is commonly used to activate surface carboxyl groups for protein immobilization.

Is CFR7UM-10 suitable for flow cytometry?

Yes. Detector settings, excitation compatibility, emission filters, particle concentration and gating conditions should be optimized.

Can CFR7UM-10 be used for phagocytosis studies?

Yes. The 7 μm size can be evaluated in macrophage uptake and particle–immune cell interaction research.

Is CFR7UM-10 suitable for lateral flow assays?

The 7 μm particles are generally too large for conventional nitrocellulose membrane migration. Smaller fluorescent particles are usually more suitable for standard LFIA formats.

How should CFR7UM-10 be dispersed?

Use gentle inversion, controlled vortexing or compatible bath sonication while avoiding excessive foam and particle damage.

Can CFR7UM-10 be customized?

Yes. Particle size, fluorescence wavelength, carboxyl density, solid content, buffer, surface ligand and packaging can be customized.

Request a Sample or Quotation

Contact SHBC for CFR7UM-10 samples, technical specifications, conjugation support and bulk pricing.

Please provide:

• Target application

• Target biomolecule

• Detection instrument

• Required quantity

• Preferred packaging

• Estimated annual demand

Product: 7um Carboxyl Red-Fluo Microspheres
Catalog Number: CFR7UM-10
Particle Size: 7 μm
Solid Content: 1%
Excitation/Emission: 535/610 nm
Surface Group: Carboxyl, COOH
Brand: SHBC
Manufacturer: Shanghai SanYu Biotechnology Co., Ltd.