800um Amino Silica Microspheres ASi800UM-10 5%

800um Amino Silica Microspheres for Immunoassay R&D

SHBC ASi800UM-10 Amino Silica Microspheres are large silica particles with a nominal diameter of 800 µm, a 5% solid content and amino-functionalized surfaces.

The large particle size supports convenient handling, filtration and solid-phase separation. Surface amino groups provide reactive sites for immobilizing antibodies, antigens, proteins, peptides and other biomolecules.

ASi800UM-10 is designed for immunoassay research, solid-phase carrier development, biomolecule coupling and batch production.

Product Specifications

Dispersion medium, amino group density, particle size distribution and storage conditions should be confirmed in the batch-specific specification or Certificate of Analysis.

Key Advantages

Large and Easy-to-Handle Particles

The 800 µm particle size supports convenient visual observation, transfer, washing and recovery during laboratory and pilot-scale processes.

Reactive Amino Surface

Surface amino groups provide sites for chemical crosslinking and covalent immobilization of compatible biomolecules.

Easy Solid-Phase Separation

The microspheres can be retained using suitable filters, meshes, columns or reaction chambers without requiring magnetic separation.

Stable Silica Matrix

The silica structure provides good mechanical and chemical stability for repeated washing, surface modification and reagent development.

Suitable for Scale-Up

SHBC supports product evaluation, pilot production, customized packaging and bulk manufacturing for research institutions and reagent developers.

Amino Surface for Biomolecule Immobilization

The amino-functionalized surface can be evaluated for immobilizing:

• Antibodies

• Antigens

• Proteins

• Peptides

• Enzymes

• Oligonucleotides

• Fluorescent labels

• Biotin derivatives

• Affinity ligands

Amine-modified silica microspheres normally require an appropriate chemical linker or activated biomolecule for stable covalent immobilization. The coupling method should be selected according to the reactive groups on the target molecule. l coupling routes include:

• Glutaraldehyde-mediated coupling

• NHS ester reactions

• Isothiocyanate reactions

• Carbodiimide-assisted coupling

• Heterobifunctional linker chemistry

Coupling efficiency, biomolecule activity and nonspecific adsorption should be experimentally optimized.

Immunoassay and Bioseparation Applications

ASi800UM-10 can be evaluated for:

• Solid-phase immunoassay development

• Antibody immobilization

• Antigen coating

• Protein capture research

• Affinity separation

• Filtration-assisted immunoreactions

• Column-based assay development

• Flow-through detection systems

• Biomolecule coupling studies

• Enzyme immobilization

• Biosensor carrier development

• Diagnostic reagent raw material research

• Protein and antibody purification research

• Laboratory bioseparation processes

Large amine-functionalized silica particles are commercially positioned for protein and antibody purification, nucleic acid purification and cell isolation applications. Compatibility with each assay format must be verified by the customer. hoose 800um Amino Silica Microspheres?

The 800 µm size is suitable for systems where convenient recovery and fixed solid-phase operation are more important than stable suspension.

Compared with smaller microspheres, ASi800UM-10 offers:

• Easier filtration and recovery

• Faster sedimentation

• Convenient manual handling

• Easy visual process monitoring

• Reduced dependence on centrifugation

• Suitability for mesh-retained systems

• Compatibility with columns and cartridges

• Convenient washing and buffer exchange

• Flexible amino surface chemistry

Because 800 µm particles are much larger than microspheres normally used in suspension immunoassays, equipment and assay compatibility should be confirmed before scale-up.

Recommended Separation Methods

Filtration

Use a filter with a pore size smaller than the microsphere diameter to retain the particles during washing and buffer exchange.

Mesh Retention

A suitable mesh or reaction chamber can hold the microspheres while allowing liquid reagents to pass through.

Sedimentation

The large particle size may support gravity-assisted settling in selected laboratory processes.

Packed Columns

The particles can be evaluated as a fixed solid phase in columns, cartridges or flow-through devices.

The selected separation method should minimize particle loss and maintain consistent contact between the microspheres and sample.

Handling and Assay Optimization

Mix the suspension gently before sampling.

Recommended precautions include:

• Avoid allowing the microspheres to dry.

• Use wide-opening containers and transfer tools.

• Avoid narrow pipette tips and tubing.

• Confirm that dispensing equipment can handle 800 µm particles.

• Use gentle mixing to reduce particle damage.

• Select filters and meshes with suitable pore sizes.

• Optimize reaction time and mixing efficiency.

• Confirm buffer and preservative compatibility.

• Evaluate blocking conditions after biomolecule coupling.

• Avoid freezing unless permitted by the batch instructions.

Before scale-up, customers should confirm particle transfer, sedimentation, washing efficiency, biomolecule loading and final assay performance.

Customization and Bulk Production

Shanghai SanYu Biotechnology Co., Ltd. provides amino silica microsphere manufacturing services for biotechnology companies, research institutions and diagnostic reagent developers.

Available customization may include:

• Different particle sizes

• Different solid contents

• Amino group density adjustment

• Custom dispersion media

• Additional surface functionalization

• Protein or antibody conjugation

• Custom packaging volumes

• Pilot-scale production

• Bulk manufacturing

• OEM and ODM services

Customization feasibility depends on the required particle size, surface chemistry, concentration, packaging and order volume.

Why Choose SHBC?

• Specialized microsphere manufacturer

• Large 800 µm silica particle specification

• Amino-functionalized surface

• Standard 5% solid content

• Suitable for solid-phase carrier research

• Convenient filtration and recovery

• Custom surface modification available

• Laboratory samples and pilot orders

• Stable batch manufacturing

• Bulk supply capability

• Custom packaging

• OEM and ODM support

Quality Documentation

Available batch documentation may include:

• Certificate of Analysis

• Safety Data Sheet

• Product specification

• Particle size information

• Solid-content information

• Batch identification

• Handling recommendations

• Storage guidance

Please confirm the available testing items before placing a bulk order.

Frequently Asked Questions

What is ASi800UM-10?

ASi800UM-10 is an 800 µm amino-functionalized silica microsphere suspension with a solid content of 5%, supplied by SHBC.

What is the main advantage of 800um particles?

The large particle size supports convenient filtration, washing, visual handling and recovery during solid-phase processes.

Can antibodies be attached to the surface?

Yes. Antibodies and other biomolecules can be evaluated for covalent immobilization using suitable amine-reactive coupling chemistry.

Can the microspheres be used in suspension immunoassays?

The 800 µm particles sediment faster than conventional immunoassay microspheres. They are more suitable for fixed, filtered or packed solid-phase formats.

How can the particles be separated?

Filtration, mesh retention, sedimentation or packed-column separation can be evaluated.

Is a crosslinker required?

A suitable linker or activated biomolecule is normally required for stable covalent coupling to the amino surface.

Can SHBC customize the amino group density?

Custom surface chemistry and amino group density can be discussed according to the application and order volume.

Are bulk quantities available?

Yes. SHBC supports sample evaluation, pilot orders and bulk production.

Is ASi800UM-10 a finished diagnostic reagent?

No. It is a raw material for research and reagent development. Customers must complete application-specific validation.

Request Samples and Bulk Pricing

Contact SHBC for ASi800UM-10 samples, product specifications, customized surfaces and bulk quotations.

Please provide:

• Required quantity

• Target application

• Target biomolecule

• Preferred coupling chemistry

• Packaging requirements

• Expected annual demand

Product: 800um Amino Silica Microspheres
Catalog Number: ASi800UM-10
Particle Size: 800 µm
Solid Content: 5%
Surface Group: Amino, –NH₂
Brand: SHBC
Manufacturer: Shanghai SanYu Biotechnology Co., Ltd.
Intended Use: Research and Reagent Development Use