80um Hydroxypropyl Dextran Gel Filtration Fillers TopGF LH-20

80 μm Hydroxypropyl Dextran Gel Filtration Media TopGF LH-20

SHBC TopGF LH-20 is an 80 μm hydroxypropyl dextran gel filtration medium supplied as a dry powder.

The crosslinked hydroxypropyl dextran matrix provides both hydrophilic and lipophilic characteristics, making TopGF LH-20 suitable for size-exclusion and solvent-dependent separation of natural products, small molecules, lipids and other organic compounds.

It is designed for laboratory purification, process development and scalable chromatography applications.

TopGF LH-20 Technical Specifications

What Is Hydroxypropyl Dextran LH-20?

LH-20 type chromatography media are based on crosslinked dextran modified with hydroxypropyl groups.

Hydroxypropyl modification gives the dextran matrix both hydrophilic and lipophilic characteristics. This enables the swollen chromatography beads to operate in aqueous mixtures and various polar organic solvent systems.

Compared with conventional aqueous gel filtration media, LH-20 is especially useful for purification of natural products and relatively small organic molecules.

How Does TopGF LH-20 Separation Work?

TopGF LH-20 can provide more than one chromatographic separation mechanism depending on the sample and mobile phase.

Size Exclusion

Molecules can be separated according to their ability to enter the porous dextran network.

Larger molecules have less access to the internal pores and generally move through the column more rapidly, while smaller molecules penetrate the pores to a greater extent.

Partition-Based Separation

Because hydroxypropyl dextran has both hydrophilic and lipophilic character, interactions between the sample, stationary phase and solvent can also contribute to separation.

This makes LH-20 useful for closely related compounds that may be difficult to resolve by molecular size alone. Cytiva specifically describes both molecular sizing and partition behavior for LH-20 depending on the solvent system.

Key Advantages of TopGF LH-20

• 80 μm nominal dry particle size

• Hydroxypropyl crosslinked dextran matrix

• Supplied as dry powder

• Hydrophilic and lipophilic characteristics

• Suitable for size-exclusion separation

• Suitable for solvent-dependent partition separation

• Compatible with aqueous and selected organic solvent systems

• Suitable for natural product purification

• Suitable for small-molecule fractionation

• Suitable for laboratory and process-scale development

• Bulk supply available

Natural Product Purification

LH-20 chromatography media are widely associated with purification and fractionation of natural products.

Typical applications may include:

• Plant extracts

• Traditional Chinese medicine extracts

• Herbal extracts

• Natural pigments

• Terpenoids

• Steroids

• Lipids

• Natural bioactive compounds

• Secondary metabolites

• Complex organic extracts

Cytiva specifically positions LH-20 for preparative purification of natural products including steroids, terpenoids and lipids, and also identifies traditional Chinese medicine purification as an application area.

Small Molecule and Biomolecule Applications

TopGF LH-20 may also be evaluated for separation of:

• Low-molecular-weight peptides

• Hormones

• Vitamins

• Lipids

• Closely related organic molecules

• Small bioactive compounds

• Reaction products

• Organic impurities

• Purification intermediates

Sigma-Aldrich describes LH-20 as suitable for fractionation of lipids, hormones, vitamins and other small biomolecules, as well as low-molecular-weight peptides.

Organic Solvent Chromatography

One of the key advantages of hydroxypropyl dextran LH-20 is its ability to swell and function in a range of aqueous and organic solvent systems.

This makes the medium especially useful for samples that have limited water solubility or require organic mobile phases.

Potential solvent systems may include:

• Water

• Methanol

• Ethanol-based systems

• Aqueous-organic mixtures

• Other validated polar organic solvents

The degree of swelling and chromatographic behavior can change with solvent composition, so column parameters should be optimized for the selected mobile phase. Cytiva notes that both particle size after swelling and exclusion behavior depend on the solvent used.

Why Choose 80 μm TopGF LH-20?

The 80 μm nominal dry particle format provides a practical balance between chromatographic resolution and column handling.

Potential benefits include:

• Efficient column packing

• Suitable flow characteristics

• Good contact between sample and porous matrix

• Practical preparative chromatography

• Suitable for laboratory scale-up

• Convenient dry-powder storage and transportation

Particle dimensions change after swelling, so 80 μm should be treated as the nominal dry particle size rather than the final swollen particle diameter.

For comparison, Cytiva's commercial LH-20 is reported at approximately 70 μm average particle size dry and approximately 103 μm after swelling in methanol.

General TopGF LH-20 Preparation Workflow

1. Select the Mobile Phase

Choose an aqueous, organic or mixed solvent according to sample solubility and desired separation mechanism.

2. Swell the Dry Media

Add TopGF LH-20 dry powder to the selected solvent and allow sufficient time for complete swelling.

3. Prepare the Slurry

Prepare a homogeneous slurry while avoiding aggressive mixing that could damage swollen particles.

4. Pack the Column

Transfer the slurry into a suitable chromatography column and allow the bed to form uniformly.

5. Equilibrate the Column

Equilibrate with the selected mobile phase before sample loading.

6. Load the Sample

Apply the sample in a concentrated volume appropriate for the desired resolution.

7. Elute and Collect Fractions

Run the selected mobile phase and collect fractions according to the purification method.

8. Analyze the Fractions

Evaluate fractions using UV, HPLC, LC-MS or another suitable analytical method.

Commercial LH-20 media are likewise supplied as dry powder and require swelling before use; Cytiva recommends swelling its own LH-20 product in the intended solvent before column packing.

How to Select the Mobile Phase

Mobile-phase selection is one of the most important parameters in LH-20 chromatography.

Consider:

• Sample solubility

• Molecular size

• Compound polarity

• Hydrophobicity

• Desired selectivity

• Target purity

• Sample loading

• Detection method

Changing the solvent can alter both bead swelling and interactions between the compound and hydroxypropyl dextran matrix.

For difficult separations, solvent composition can be optimized experimentally to improve selectivity.

TopGF LH-20 vs. Conventional Dextran G-25 Media

LH-20 is therefore better positioned for natural-product and organic-solvent chromatography, while G-25 type media are generally more strongly associated with aqueous desalting and buffer-exchange workflows.

Typical Industries

TopGF LH-20 can be evaluated in:

• Pharmaceutical research

• Natural product research

• Traditional Chinese medicine research

• Herbal extract purification

• Biochemical research

• Food and nutraceutical research

• Fine chemical purification

• Academic laboratories

• Preparative chromatography

• Process development

Process Development Considerations

For reproducible chromatography, optimize:

• Dry media quantity

• Swelling solvent

• Swelling time

• Column dimensions

• Bed height

• Sample concentration

• Sample volume

• Mobile-phase composition

• Flow rate

• Fraction volume

Because swelling depends on solvent type, process scale-up should preferably use the same or a closely controlled solvent system used during method development.

Quality Control for Bulk Production

For chromatography process development, important quality parameters may include:

• Dry particle-size consistency

• Bead morphology

• Moisture control

• Swelling behavior

• Bed-volume consistency

• Chromatographic performance

• Flow characteristics

• Batch-to-batch consistency

• Packaging integrity

Why Choose SHBC TopGF LH-20?

• 80 μm nominal dry particle size

• Hydroxypropyl crosslinked dextran matrix

• Dry-powder format

• Suitable for natural product purification

• Suitable for small-molecule fractionation

• Suitable for aqueous-organic chromatography

• Laboratory sample evaluation

• Bulk manufacturing capability

• Scale-up supply

• Custom packaging support

Storage and Handling

Recommended practices include:

• Store the dry powder in a sealed container.

• Protect from excess moisture.

• Avoid contamination during handling.

• Use clean and chemically compatible equipment.

• Fully swell the media before column packing.

• Avoid aggressive mechanical stirring after swelling.

• Validate solvent compatibility before use.

Frequently Asked Questions

What is TopGF LH-20?

TopGF LH-20 is an 80 μm nominal dry hydroxypropyl crosslinked dextran chromatography medium supplied as a dry powder.

What is hydroxypropyl dextran?

It is a modified dextran matrix containing hydroxypropyl groups that provide both hydrophilic and lipophilic characteristics.

What is TopGF LH-20 mainly used for?

It is designed for purification and fractionation of natural products, small molecules, lipids, steroids, terpenoids and related compounds.

Is TopGF LH-20 a size-exclusion resin?

Yes. Size exclusion is an important separation mechanism, but solvent-dependent partition effects may also contribute to selectivity.

Can LH-20 be used with organic solvents?

Yes. Hydroxypropyl dextran LH-20 media are specifically associated with chromatography in aqueous-organic and polar organic solvent systems.

Is the particle size 80 μm after swelling?

No. The specified 80 μm is the nominal dry particle size. The particle size can increase after swelling, and the final size depends on the solvent system.

Is TopGF LH-20 supplied pre-swollen?

No. TopGF LH-20 is supplied as dry powder and should be properly swollen before column packing.

Can it be used for traditional Chinese medicine purification?

It may be evaluated for fractionation and purification of complex TCM and herbal extracts. Commercial LH-20 media are also specifically positioned for natural-product and TCM purification.

Can it separate steroids and terpenoids?

Yes. LH-20 type media are commonly used for purification of steroids, terpenoids and other natural organic compounds.

Can TopGF LH-20 be used for protein desalting?

For routine protein desalting and buffer exchange, G-25 type dextran gel filtration media are generally a better choice. LH-20 is better positioned for natural products, small molecules and solvent-based chromatography.

Can SHBC provide bulk quantities?

Yes. SHBC supports laboratory evaluation, process development and bulk supply of TopGF LH-20 chromatography media.

Request TopGF LH-20 Samples and Bulk Pricing

Contact Shanghai SanYu Biotechnology Co., Ltd. for TopGF LH-20 samples, technical information and bulk quotations.

Please provide:

• Target compound

• Sample type

• Mobile phase

• Column size

• Required quantity

• Purification scale

• Estimated annual demand

SHBC TopGF LH-20 is intended for research, purification and process-development applications.