8um Carboxyl Green-Fluo Microspheres CFG8UM-10 1%

8um Carboxyl Green-Fluo Microspheres CFG8UM-10

SHBC CFG8UM-10 Carboxyl Green-Fluo Microspheres are 8um fluorescent polymer particles with carboxyl-functionalized surfaces.

With excitation at 470/488nm and emission at 525nm, the microspheres provide a bright green fluorescent signal for flow cytometry, fluorescence microscopy, cell tracking, biomolecule conjugation and particle-based assays.

Product Specifications

Key Product Advantages

• Bright green fluorescence at 525nm

• Compatible with 470nm and 488nm excitation

• Reactive carboxyl surface

• High fluorescence signal per particle

• Easy observation under fluorescence microscopy

• Large surface area for biomolecule loading

• Suitable for flow cytometry and cell research

• Available for research and batch production

Why Choose 8um Green Fluorescent Microspheres?

The 8um particle size provides easy optical identification and a strong fluorescent signal from each particle.

Compared with smaller fluorescent microspheres, CFG8UM-10 offers:

• Easier particle counting and tracking

• Stronger fluorescence per particle

• More surface area for ligand immobilization

• Clear discrimination in flow cytometry

• Convenient microscopic observation

• Good suitability for cell–particle interaction studies

Carboxyl Surface for Covalent Coupling

CFG8UM-10 contains reactive carboxyl groups for the covalent immobilization of amine-containing biomolecules.

Compatible ligands include:

• Antibodies

• Antigens

• Proteins

• Peptides

• Enzymes

• Oligonucleotides

• Other amino-containing molecules

The carboxyl groups can be activated using EDC/NHS chemistry to form stable amide bonds with target biomolecules.

Main Applications

Flow Cytometry

The 8um particle size can be clearly identified by suitable flow cytometry systems.

Typical applications include:

• Fluorescent particle analysis

• Particle counting

• Binding assay development

• Fluorescence channel evaluation

• Microsphere population discrimination

• Flow cytometry method development

Fluorescence Microscopy

CFG8UM-10 provides a visible green fluorescent signal for microscopic observation.

It can be used for:

• Particle localization

• Fluorescence imaging

• Particle movement analysis

• Surface binding studies

• Material distribution research

• Microfluidic visualization

Cell Uptake and Phagocytosis Studies

The 8um particle size is suitable for studying interactions between fluorescent particles and selected cell types.

Potential uses include:

• Phagocytosis research

• Cell–particle binding

• Particle uptake studies

• Immune cell research

• Intracellular localization

• Particle transport analysis

Particle concentration and incubation conditions should be optimized for each cell model.

Fluorescence Immunoassays

Antibodies, antigens or proteins can be immobilized on the carboxyl surface to prepare fluorescent capture particles.

CFG8UM-10 can be evaluated for:

• Particle-based immunoassays

• Sandwich binding assays

• Competitive assays

• Antibody detection

• Antigen detection

• Fluorescent agglutination research

Multiplex Suspension Assays

Different particle sizes, fluorescence signals or surface ligands can be used to create distinguishable microsphere populations.

Potential applications include:

• Multiplex immunoassays

• Suspension arrays

• Liquid-phase biochips

• Protein detection panels

• Nucleic acid hybridization research

Biosensors and Microfluidics

The carboxyl surface supports biological ligand immobilization for biosensors, microarrays, biochips and microfluidic systems.

The green fluorescence helps visualize particle capture, movement and distribution.

Particle Tracking and Tracing

CFG8UM-10 can be used as a fluorescent tracer in material transport, filtration, fluid movement and particle distribution studies.

Recommended Coupling Workflow

1. Mix the microsphere suspension thoroughly.

2. Wash the microspheres with a suitable activation buffer.

3. Activate the surface carboxyl groups using EDC and NHS.

4. Add the antibody, protein or target ligand.

5. Incubate under optimized reaction conditions.

6. Block the remaining active sites.

7. Wash the conjugated microspheres.

8. Resuspend in a suitable storage buffer.

9. Evaluate coupling efficiency and fluorescence performance.

Reaction pH, reagent concentration, coupling time and blocking conditions should be optimized for each biomolecule.

Handling Recommendations

• Mix gently before use.

• Protect the product from strong light.

• Avoid prolonged high-power ultrasonication.

• Do not allow the microspheres to dry.

• Avoid repeated freezing and thawing.

• Use clean, low-binding consumables.

• Confirm buffer compatibility before scale-up.

• Follow the product label for storage conditions.

Application Notes

For flow cytometry, optimize:

• Particle concentration

• Sample dispersion

• Flow rate

• Detector settings

• Fluorescence filters

• Gating strategy

For cell studies, optimize the particle-to-cell ratio, incubation time and washing conditions.

Because 8um particles are larger than fluorescent nanoparticles commonly used in lateral flow tests, membrane migration must be validated before use in lateral flow applications.

Customization and Bulk Supply

SHBC supports fluorescent microsphere projects from laboratory evaluation to batch manufacturing.

Customization options include:

• Different particle sizes

• Different fluorescence wavelengths

• Fluorescence intensity adjustment

• Surface carboxyl density adjustment

• Alternative functional groups

• Solids-content adjustment

• Custom suspension buffers

• Custom packaging

• OEM and ODM production

Why Choose SHBC?

Shanghai SanYu Biotechnology Co., Ltd. manufactures functional microspheres and magnetic beads for fluorescence detection, immunoassays, bioseparation and biological research.

SHBC provides:

• Controlled particle-size production

• Stable fluorescence performance

• Functional surface modification

• Reliable batch-to-batch consistency

• Biomolecule coupling support

• Research samples

• Flexible packaging

• Pilot-scale and bulk production

Frequently Asked Questions

What is CFG8UM-10?

CFG8UM-10 is an 8um carboxyl-functionalized green fluorescent microsphere suspension with 1% solids content.

What are the excitation and emission wavelengths?

The recommended excitation wavelengths are 470nm and 488nm. The emission wavelength is 525nm.

Is CFG8UM-10 compatible with a 488nm laser?

Yes. The microspheres can be excited using a suitable 488nm laser and detected through a compatible green fluorescence channel.

Can CFG8UM-10 be coupled with antibodies?

Yes. Its surface carboxyl groups can be activated using EDC/NHS chemistry for covalent antibody or protein conjugation.

Is it suitable for flow cytometry?

Yes. The 8um particle size and green fluorescence are suitable for flow cytometry research, particle counting and binding assay development.

Can it be used for fluorescence microscopy?

Yes. Its micrometer-scale size and bright fluorescence support easy visualization, localization and particle tracking.

Can it be used for phagocytosis studies?

CFG8UM-10 can be evaluated for phagocytosis, cell uptake and cell–particle interaction research.

Is it suitable for lateral flow assays?

The 8um particle size is larger than particles commonly used in lateral flow tests. Membrane migration and assay compatibility must be validated.

Can SHBC provide samples and bulk quantities?

Yes. SHBC supports research samples, pilot-scale evaluation and bulk manufacturing.

Can the product be customized?

Yes. Particle size, fluorescence wavelength, surface functional group, solids content, buffer and packaging can be customized.

Ordering Information

Product Name: 8um Carboxyl Green-Fluo Microspheres
Catalog Number: CFG8UM-10
Particle Size: 8um
Solids Content: 1%
Excitation Wavelength: 470/488nm
Emission Wavelength: 525nm
Surface Group: Carboxyl, COOH
Brand: SHBC
Manufacturer: Shanghai SanYu Biotechnology Co., Ltd.

Contact SHBC for samples, technical documents, customized specifications and bulk production quotations.

For research and manufacturing use. Performance in the final application should be validated by the customer.