8um Carboxyl Red-Fluo Microspheres CFR8UM-10

8um Carboxyl Red-Fluo Microspheres CFR8UM-10

SHBC CFR8UM-10 are 8 μm carboxyl-functionalized red fluorescent microspheres developed for flow cytometry, fluorescence microscopy, cell interaction studies and biomolecule immobilization.

The microspheres feature excitation at 535 nm, emission at 610 nm and 1% solid content. Surface carboxyl groups support covalent coupling with antibodies, antigens, proteins and peptides.

Product Specifications

Batch-specific particle size, particle size distribution, carboxyl content and fluorescence data are available in the Certificate of Analysis.

Red Fluorescence at 535/610nm

CFR8UM-10 produces red fluorescence with excitation at approximately 535 nm and emission centered at approximately 610 nm.

The fluorescence profile is suitable for compatible flow cytometers, fluorescence microscopes, imaging systems and fluorescence readers.

Key Advantages

Uniform 8um Particle Size

Controlled particle sizing supports repeatable fluorescence detection, surface coupling and particle analysis.

Readily Detectable Fluorescence

The micrometer-scale particles provide a clear fluorescent signal for imaging and flow-based detection.

Reactive Carboxyl Surface

Surface COOH groups support covalent immobilization of antibodies, antigens, proteins, peptides and other amino-containing molecules.

Large Coupling Surface

The 8 μm particle diameter provides substantial surface area per particle for biomolecule immobilization.

Easy Particle Recovery

The micrometer-scale particles can be collected by centrifugation during washing and conjugation procedures.

Custom and Bulk Supply

SHBC supports laboratory samples, customized specifications, pilot production and bulk manufacturing.

Main Applications

Flow Cytometry

CFR8UM-10 can be evaluated for particle detection, fluorescence channel testing and flow-based assay development.

Fluorescence Microscopy

The red fluorescent signal supports particle localization, distribution and imaging studies.

Phagocytosis Studies

The 8 μm particles can be used in macrophage uptake, phagocytosis and immune-cell interaction research.

Cell–Particle Interaction

Suitable for studying particle adhesion, cellular contact, surface recognition and uptake.

Particle-Based Immunoassays

Antibodies or antigens can be immobilized on the carboxyl surface for suspension immunoassays and agglutination studies.

Biomolecule Immobilization

Suitable for coupling proteins, enzymes, peptides and other molecules containing primary amine groups.

Microfluidic and Flow Research

Can be evaluated in particle transport, channel visualization, filtration and fluid-flow studies.

Instrument Evaluation

Suitable for selected fluorescence detection, particle counting and imaging method development.

Biomolecule Coupling

Surface carboxyl groups can be activated using EDC/NHS chemistry and coupled with primary amine groups on antibodies or proteins.

A typical coupling workflow includes:

1. Wash and redisperse the microspheres.

2. Activate the carboxyl groups with EDC and NHS.

3. Add the antibody or target protein.

4. Block remaining active sites.

5. Wash and resuspend the conjugated particles.

6. Evaluate coupling and fluorescence performance.

Activation pH, reagent concentration, protein loading and reaction time should be optimized for each biomolecule.

Coupling Benefits

CFR8UM-10 provides:

• Reactive surface carboxyl groups

• Large surface area per particle

• Stable covalent immobilization

• Easy centrifugation and washing

• Clear fluorescence detection

• Flexible ligand modification

• Compatibility with bead-based assays

• Custom conjugation support

Flow Cytometry Considerations

Recommended parameters to optimize include:

• Particle concentration

• Sample flow rate

• Excitation source

• Emission filter

• FSC and SSC settings

• Detector voltage

• Gating strategy

• Doublet discrimination

• Particle aggregation

Select a fluorescence channel compatible with emission near 610 nm.

Cell and Phagocytosis Studies

For cellular studies, evaluate:

• Particle-to-cell ratio

• Incubation time

• Cell type

• Particle surface modification

• Culture conditions

• Washing efficiency

• Internalization controls

• Background fluorescence

Include untreated cells, particle-only samples and suitable fluorescence controls.

The product should be evaluated for compatibility with the selected cell model before use.

Dispersion and Handling

Before use:

• Mix by gentle inversion or controlled vortexing

• Redisperse settled particles completely

• Avoid excessive bubbles and foam

• Protect the suspension from strong light

• Prevent the particles from drying

• Avoid repeated freeze-thaw cycles

• Confirm buffer compatibility

• Use clean laboratory equipment

Controlled bath sonication may be used to break weak aggregates when required.

Storage Recommendations

Keep the container tightly closed and protected from light.

Do not return unused suspension to the original container. Refer to the product label, Technical Data Sheet and Certificate of Analysis for batch-specific storage conditions.

Custom Carboxyl Fluorescent Microspheres

SHBC can customize fluorescent microspheres according to research and production requirements.

Customization options may include:

• Custom particle size

• Custom fluorescence wavelength

• Different fluorescence colors

• Carboxyl group density

• Solid content

• Dispersion buffer

• Surface modification

• Biomolecule conjugation

• Custom packaging

• OEM and ODM production

Please provide the target application, required wavelength, surface ligand and order quantity when requesting customization.

Quality Control and Documents

Available product documents may include:

• Certificate of Analysis

• Technical Data Sheet

• Safety Data Sheet

• Particle size report

• Particle size distribution data

• Fluorescence spectrum

• Carboxyl content data

• Microscopy images

• Batch inspection report

Why Choose SHBC?

Shanghai SanYu Biotechnology Co., Ltd. supplies fluorescent microspheres, colored latex particles, magnetic beads and functional microsphere materials.

SHBC provides:

• Controlled microsphere manufacturing

• Stable particle size distribution

• Multiple fluorescence wavelengths

• Functional surface modification

• Batch-to-batch consistency

• Sample evaluation support

• Pilot and bulk production

• OEM and ODM services

Frequently Asked Questions

What is CFR8UM-10?

CFR8UM-10 is an 8 μm carboxyl-functionalized red fluorescent microsphere suspension with 1% solid content.

What are the excitation and emission wavelengths?

The excitation wavelength is 535 nm and the emission wavelength is 610 nm.

What is CFR8UM-10 mainly used for?

It is suitable for flow cytometry, fluorescence microscopy, phagocytosis research, cell–particle interaction and biomolecule coupling.

Can antibodies be coupled to CFR8UM-10?

Yes. Antibodies and proteins containing primary amine groups can be covalently coupled to the carboxyl surface.

Which coupling chemistry is recommended?

EDC/NHS chemistry is commonly used to activate surface carboxyl groups for protein immobilization.

Is CFR8UM-10 suitable for flow cytometry?

Yes. Excitation compatibility, emission filters, scatter settings, particle concentration and gating conditions should be optimized.

Can CFR8UM-10 be used for phagocytosis studies?

Yes. The 8 μm particles can be evaluated in macrophage uptake and particle–immune cell interaction studies.

Is CFR8UM-10 suitable for lateral flow assays?

The 8 μm particles are generally too large for conventional nitrocellulose membrane migration. Smaller fluorescent particles are usually more suitable for standard LFIA formats.

How should CFR8UM-10 be dispersed?

Use gentle inversion, controlled vortexing or compatible bath sonication while avoiding excessive foam and particle damage.

Can CFR8UM-10 be customized?

Yes. Particle size, fluorescence wavelength, carboxyl density, solid content, buffer, surface ligand and packaging can be customized.

Are samples and bulk quantities available?

Yes. SHBC supports laboratory samples, pilot orders and industrial batch production.

Request a Sample or Quotation

Contact SHBC for CFR8UM-10 samples, technical specifications, conjugation support and bulk pricing.

Please provide:

• Target application

• Target biomolecule

• Detection instrument

• Required quantity

• Preferred packaging

• Estimated annual demand

Product: 8um Carboxyl Red-Fluo Microspheres
Catalog Number: CFR8UM-10
Particle Size: 8 μm
Solid Content: 1%
Excitation/Emission: 535/610 nm
Surface Group: Carboxyl, COOH
Brand: SHBC
Manufacturer: Shanghai SanYu Biotechnology Co., Ltd.