Product Overview
SHBC CNBr TopAC 4B is a cyanogen bromide-activated agarose gel designed for the covalent immobilization of biomolecules containing primary amine (-NH2) groups.
According to the supplied product information, biomolecules containing primary amines can be covalently coupled to the activated matrix. This allows CNBr TopAC 4B to be used for the preparation of specific affinity chromatography media and immobilized enzymes.
The specified coupling density is 25–60 mg/mL using α-lactalbumin as the reference protein.
Key Features
Cyanogen bromide-activated agarose gel
Pre-activated affinity chromatography resin
Designed for biomolecules containing primary amine (-NH2) groups
Covalent biomolecule immobilization
Coupling density: 25–60 mg/mL (α-Lactalbumin)
Suitable for preparation of specific affinity media
Suitable for enzyme immobilization
Available in both weight and volume packaging
Package sizes up to 10 kg and 20 L
Technical Specifications
Parameter | Specification |
|---|
Product Name | CNBr TopAC 4B |
Brand | SHBC |
English Name | CNBr Pre-activated Affinity Resin 4B |
Series | 4B |
Activation | Cyanogen Bromide Activated |
Matrix | Activated Agarose Gel |
Coupling Group | Primary Amine (-NH2) Containing Biomolecules |
Coupling Density | 25–60 mg/mL (α-Lactalbumin) |
Product Type | Pre-activated Affinity Resin |
Main Application | Biomolecule Coupling / Enzyme Immobilization |
Weight Package | 25 g / 100 g / 500 g / 1 kg / 5 kg / 10 kg |
Volume Package | 25 mL / 100 mL / 300 mL / 1 L / 5 L / 10 L / 20 L |
SHBC Art.No. | Packaging Specification |
|---|
A5148 | 25 g |
A5149 | 100 g |
A5150 | 500 g |
A5151 | 1 kg |
A5152 | 5 kg |
A5153 | 10 kg |
A5154 | 25 mL |
A5155 | 100 mL |
A5156 | 300 mL |
A5157 | 1 L |
A5158 | 5 L |
A5159 | 10 L |
A5160 | 20 L |
Cyanogen Bromide-Activated Agarose
CNBr TopAC 4B is a cyanogen bromide-activated agarose gel.
The activated matrix allows biomolecules containing suitable primary amine groups to be covalently immobilized onto the agarose support.
This provides a pre-activated matrix for preparing customized affinity chromatography media.
Primary Amine (-NH2) Coupling
According to the supplied product information, biomolecules containing primary amine (-NH2) groups can be covalently coupled to CNBr TopAC 4B.
This allows users to immobilize suitable biomolecules onto the agarose matrix for downstream affinity chromatography or immobilization applications.
Coupling Density
The specified coupling density of CNBr TopAC 4B is:
25–60 mg/mL (α-Lactalbumin)
The supplied table specifically uses α-lactalbumin as the reference protein.
Therefore, 25–60 mg/mL should be presented as a coupling density, not as dynamic binding capacity.
CNBr TopAC 4B can be used to prepare specific affinity chromatography media.
A suitable biomolecule containing primary amine groups can be covalently coupled to the activated agarose matrix, creating a customized affinity medium for subsequent separation or purification applications.
Enzyme Immobilization
The supplied product information specifically identifies immobilized enzyme preparation as an application of CNBr TopAC 4B.
Enzymes containing suitable primary amine groups can be covalently coupled to the activated agarose matrix.
Biomolecule Immobilization
CNBr TopAC 4B provides an activated solid support for covalent biomolecule immobilization.
The key functional requirement stated in the supplied information is that the biomolecule contains primary amine (-NH2) groups suitable for coupling to the activated matrix.
Applications
SHBC CNBr TopAC 4B can be used for:
Biomolecule immobilization
Primary amine (-NH2) coupling
Affinity ligand immobilization
Preparation of specific affinity media
Customized affinity chromatography resin preparation
Enzyme immobilization
Immobilized enzyme preparation
Affinity chromatography research
Product Advantages
CNBr TopAC 4B provides:
Pre-activated agarose matrix
Cyanogen bromide activation
Covalent coupling of primary amine-containing biomolecules
25–60 mg/mL coupling density using α-lactalbumin
Suitable for customized affinity media preparation
Suitable for immobilized enzyme preparation
Both weight and volume packaging options
Scale range from 25 g to 10 kg and 25 mL to 20 L
CNBr Activated Resin for Biomolecule Coupling
CNBr-activated agarose provides a matrix for immobilizing biomolecules containing primary amine groups.
For CNBr TopAC 4B, the supplied specification gives a coupling density of:
25–60 mg/mL (α-Lactalbumin)
This specification provides a reference for evaluating the coupling performance of the activated matrix.
CNBr Activated Resin for Enzyme Immobilization
CNBr TopAC 4B can be used for immobilized enzyme preparation.
Suitable enzymes containing primary amine groups can be covalently coupled to the activated agarose matrix, providing an immobilized enzyme support for appropriate applications.
CNBr TopAC 4B Packaging Options
CNBr TopAC 4B is supplied in two packaging formats.
Weight packaging:
25 g / 100 g / 500 g / 1 kg / 5 kg / 10 kg
Volume packaging:
25 mL / 100 mL / 300 mL / 1 L / 5 L / 10 L / 20 L
This provides options for both laboratory-scale evaluation and larger-scale applications.
Frequently Asked Questions
What is CNBr TopAC 4B?
CNBr TopAC 4B is a cyanogen bromide-activated agarose gel for covalent coupling of biomolecules containing primary amine groups.
What is the English product name?
The supplied English name is CNBr Pre-activated Affinity Resin 4B.
What does CNBr mean?
CNBr refers to cyanogen bromide, which is used to activate the agarose matrix.
What biomolecules can be coupled to CNBr TopAC 4B?
According to the supplied information, biomolecules containing primary amine (-NH2) groups can be covalently coupled to the matrix.
What is the coupling density?
The specified coupling density is 25–60 mg/mL using α-lactalbumin as the reference protein.
Is 25–60 mg/mL the dynamic binding capacity?
No. The supplied table specifically defines 25–60 mg/mL as Coupling Density, with α-lactalbumin as the reference protein.
What is CNBr TopAC 4B used for?
It is designed for biomolecule coupling, preparation of specific affinity media and immobilized enzyme preparation.
Can CNBr TopAC 4B be used for enzyme immobilization?
Yes. Immobilized enzyme preparation is specifically stated as an application in the supplied product information.
Can it be used to prepare customized affinity chromatography resin?
Yes. Suitable biomolecules containing primary amine groups can be covalently coupled to the activated matrix to prepare specific affinity media.
Is particle size specified?
No. The supplied table does not provide a numerical particle size.
Is pressure resistance specified?
No. The supplied information does not provide a numerical pressure resistance value.
Is the recommended flow rate specified?
No. A numerical recommended flow rate is not provided in the supplied table.
What weight packages are available?
The available weight packages are 25 g, 100 g, 500 g, 1 kg, 5 kg and 10 kg.
What volume packages are available?
The available volume packages are 25 mL, 100 mL, 300 mL, 1 L, 5 L, 10 L and 20 L.
What are the SHBC Art.No. numbers?
The corresponding Art.No. are A5148–A5160.
What brand is CNBr TopAC 4B?
CNBr TopAC 4B is supplied under the SHBC brand.
