Product Overview
SHBC CNBr TopAC 4FF is a cyanogen bromide-activated agarose resin designed for covalent coupling of biomolecules containing primary amine (-NH2) groups.
According to the supplied product information, suitable biomolecules can be covalently coupled to the activated matrix for preparation of specific affinity chromatography media and immobilized enzymes.
CNBr TopAC 4FF provides a specified coupling density of 13–26 mg/mL using α-lactalbumin as the reference protein.
Key Features
CNBr pre-activated affinity resin
Cyanogen bromide-activated agarose matrix
Designed for primary amine (-NH2) coupling
Covalent biomolecule immobilization
Coupling density: 13–26 mg/mL (α-Lactalbumin)
Suitable for customized affinity media preparation
Suitable for enzyme immobilization
Weight and volume packaging available
Laboratory to large-scale package options
Technical Specifications
Parameter | Specification |
|---|
Product Name | CNBr TopAC 4FF |
Brand | SHBC |
English Name | CNBr Pre-activated Affinity Resin 4FF |
Series | 4FF |
Activation | Cyanogen Bromide Activated |
Matrix | Activated Agarose Gel |
Coupling Target | Primary Amine (-NH2) Containing Biomolecules |
Coupling Density | 13–26 mg/mL (α-Lactalbumin) |
Product Type | Pre-activated Affinity Resin |
Main Application | Biomolecule Coupling / Enzyme Immobilization |
Weight Package | 25 g / 100 g / 500 g / 1 kg / 5 kg / 10 kg |
Volume Package | 25 mL / 100 mL / 300 mL / 1 L / 5 L / 10 L / 20 L |
SHBC Art.No. | Packaging Specification |
|---|
A5161 | 25 g |
A5162 | 100 g |
A5163 | 500 g |
A5164 | 1 kg |
A5165 | 5 kg |
A5166 | 10 kg |
A5167 | 25 mL |
A5168 | 100 mL |
A5169 | 300 mL |
A5170 | 1 L |
A5171 | 5 L |
A5172 | 10 L |
A5173 | 20 L |
Cyanogen Bromide-Activated Agarose Resin
CNBr TopAC 4FF is a cyanogen bromide-activated agarose resin.
The pre-activated matrix enables biomolecules containing suitable primary amine groups to be covalently immobilized onto the agarose support.
This allows users to prepare application-specific affinity chromatography media without starting with an unactivated agarose matrix.
Primary Amine (-NH2) Coupling
According to the supplied information, biomolecules containing primary amine (-NH2) groups can be covalently coupled to CNBr TopAC 4FF.
The resulting immobilized biomolecule can then be used as part of a specific affinity medium or immobilized enzyme system.
Coupling Density
The specified coupling density of CNBr TopAC 4FF is:
13–26 mg/mL (α-Lactalbumin)
The supplied table specifically uses α-lactalbumin as the reference protein.
This value represents coupling density, rather than dynamic binding capacity.
Affinity Ligand Immobilization
CNBr TopAC 4FF can be used as a pre-activated support for immobilizing suitable affinity ligands containing primary amine groups.
Covalent immobilization onto the agarose matrix enables preparation of specific affinity chromatography media for suitable separation and purification applications.
Enzyme Immobilization
The supplied product description specifically states that CNBr-activated agarose can be used for immobilized enzyme preparation.
Suitable enzymes containing primary amine groups can be covalently coupled to CNBr TopAC 4FF to prepare an immobilized enzyme matrix.
CNBr TopAC 4FF provides a pre-activated matrix for users who need to prepare customized affinity chromatography media.
A suitable biomolecule containing primary amine groups can be coupled to the resin, creating an application-specific affinity matrix.
Applications
SHBC CNBr TopAC 4FF can be used for:
Biomolecule immobilization
Primary amine (-NH2) coupling
Affinity ligand immobilization
Customized affinity resin preparation
Specific affinity media preparation
Enzyme immobilization
Immobilized enzyme preparation
Affinity chromatography research
Product Advantages
CNBr TopAC 4FF provides:
Pre-activated agarose matrix
Cyanogen bromide activation
Covalent primary amine coupling
13–26 mg/mL coupling density (α-Lactalbumin)
Suitable for affinity ligand immobilization
Suitable for enzyme immobilization
Multiple weight and volume packages
Scale range from 25 g to 10 kg and 25 mL to 20 L
CNBr TopAC 4FF for Biomolecule Immobilization
CNBr TopAC 4FF is designed for immobilization of biomolecules containing primary amine (-NH2) groups.
The activated agarose matrix provides reactive sites that enable covalent attachment of suitable biomolecules.
This makes the product suitable as a starting matrix for preparing customized affinity chromatography media.
CNBr TopAC 4FF for Enzyme Immobilization
CNBr TopAC 4FF can also be used for enzyme immobilization.
Suitable enzymes containing primary amine groups can be covalently attached to the activated matrix, enabling preparation of immobilized enzyme systems.
CNBr TopAC 4B vs CNBr TopAC 4FF
The supplied table gives different reference coupling densities for the two products:
Product | Coupling Density | Reference Protein |
|---|
CNBr TopAC 4B | 25–60 mg/mL | α-Lactalbumin |
CNBr TopAC 4FF | 13–26 mg/mL | α-Lactalbumin |
These values should be treated as coupling density specifications, not dynamic binding capacities.
Available Package Sizes
CNBr TopAC 4FF is available in both weight and volume packaging.
Weight: 25 g / 100 g / 500 g / 1 kg / 5 kg / 10 kg
Volume: 25 mL / 100 mL / 300 mL / 1 L / 5 L / 10 L / 20 L
Frequently Asked Questions
What is CNBr TopAC 4FF?
CNBr TopAC 4FF is a cyanogen bromide-activated agarose affinity resin for covalent coupling of biomolecules containing primary amine groups.
What is the English product name?
The supplied English name is CNBr Pre-activated Affinity Resin 4FF.
What is the coupling density?
The specified coupling density is 13–26 mg/mL using α-lactalbumin as the reference protein.
Is 13–26 mg/mL the binding capacity?
No. The supplied table specifically identifies 13–26 mg/mL as Coupling Density.
What type of biomolecules can be coupled?
According to the supplied information, biomolecules containing primary amine (-NH2) groups can be covalently coupled to the matrix.
Can CNBr TopAC 4FF be used for enzyme immobilization?
Yes. The supplied description specifically identifies immobilized enzyme preparation as an application.
Yes. It can be used to covalently immobilize suitable biomolecules for preparation of specific affinity chromatography media.
What is the difference between CNBr TopAC 4B and 4FF in the supplied table?
One clear difference provided by the table is their reference coupling density: 4B is 25–60 mg/mL, while 4FF is 13–26 mg/mL, both using α-lactalbumin as the reference.
Is particle size specified?
No. The supplied information does not provide a numerical particle size.
Is pressure resistance specified?
No. A numerical pressure resistance value is not provided.
What package sizes are available?
Weight packages are 25 g, 100 g, 500 g, 1 kg, 5 kg and 10 kg. Volume packages are 25 mL, 100 mL, 300 mL, 1 L, 5 L, 10 L and 20 L.
What are the SHBC Art.No. numbers?
The corresponding Art.No. are A5161–A5173.
What brand is CNBr TopAC 4FF?
CNBr TopAC 4FF is supplied under the SHBC brand.
