Epoxy TopAC 6B Pre-activated Affinity Resin

Product Overview

SHBC Epoxy TopAC 6B is an epoxy pre-activated affinity chromatography resin based on a 6% agarose matrix.

According to the supplied product information, the resin can covalently immobilize organic molecules containing amino, thiol or hydroxyl groups through chemical reactions.

Suitable molecules include proteins, peptides and carbohydrates. The specified coupling density is 19–40 μmol epoxide/mL.

Key Features

• Epoxy pre-activated affinity resin

• 6% agarose matrix

• Coupling density: 19–40 μmol epoxide/mL

• Supports amino group coupling

• Supports thiol group coupling

• Supports hydroxyl group coupling

• Suitable for proteins, peptides and carbohydrates

• Convenient pre-activated format

• Mild coupling conditions

• High coupling efficiency

• Broad application range

• Weight and volume packaging available

Technical Specifications

Ordering Information

Epoxy Pre-activated Agarose Resin

Epoxy TopAC 6B uses an epoxy pre-activated 6% agarose matrix.

The reactive epoxide groups provide sites for covalent immobilization of suitable organic molecules through chemical coupling reactions.

The resin is supplied in a pre-activated form, eliminating the need to prepare the agarose activation chemistry before ligand coupling.

Coupling Density

The specified coupling density of Epoxy TopAC 6B is:

19–40 μmol epoxide/mL

The supplied table specifically defines this parameter as Coupling Density (μmol epoxide/mL).

It should therefore not be described as protein binding capacity or dynamic binding capacity.

Amino Group Coupling

Epoxy TopAC 6B can be used to immobilize organic molecules containing amino groups.

According to the supplied product information, amino-containing molecules can chemically react with the activated matrix and become covalently immobilized on the agarose gel.

Thiol Group Coupling

The resin also supports coupling of organic molecules containing thiol groups.

This expands the range of biomolecules and affinity ligands that can be immobilized onto the Epoxy TopAC 6B matrix.

Hydroxyl Group Coupling

Organic molecules containing hydroxyl groups can also be coupled to Epoxy TopAC 6B.

The ability to couple amino, thiol and hydroxyl groups gives the product a broad range of potential ligand immobilization applications.

Protein Immobilization

Proteins are specifically listed in the supplied product description as one of the types of organic molecules that can be immobilized onto Epoxy TopAC 6B.

Suitable proteins containing compatible functional groups can be covalently attached to the agarose matrix.

Peptide Immobilization

Epoxy TopAC 6B can also be used for peptide immobilization.

Peptides containing suitable amino, thiol or hydroxyl groups can undergo chemical coupling to the activated matrix.

Carbohydrate Immobilization

The supplied product information also specifically lists carbohydrates among the organic molecules suitable for coupling.

This broadens the potential use of Epoxy TopAC 6B beyond protein and peptide immobilization.

Affinity Ligand Immobilization

Epoxy TopAC 6B provides a pre-activated support for covalent immobilization of suitable affinity ligands.

The resulting ligand-coupled matrix can be used to prepare customized affinity chromatography media for appropriate separation and purification applications.

Mild Coupling Conditions

According to the supplied product description, one of the advantages of Epoxy TopAC 6B is its mild coupling conditions.

This characteristic supports its use for immobilization of suitable biomolecules where practical coupling conditions are important.

High Coupling Efficiency

The supplied product information also identifies high coupling efficiency as one of the characteristics of Epoxy TopAC 6B.

Together with its broad compatibility with amino, thiol and hydroxyl groups, this makes the resin suitable for diverse immobilization applications.

Applications

SHBC Epoxy TopAC 6B can be used for:

• Protein immobilization

• Peptide immobilization

• Carbohydrate immobilization

• Affinity ligand immobilization

• Amino group coupling

• Thiol group coupling

• Hydroxyl group coupling

• Customized affinity media preparation

• Biomolecule immobilization

• Affinity chromatography research

Product Advantages

Epoxy TopAC 6B provides:

• 6% agarose matrix

• Epoxy pre-activated surface

• 19–40 μmol epoxide/mL coupling density

• Amino group coupling

• Thiol group coupling

• Hydroxyl group coupling

• Suitable for proteins, peptides and carbohydrates

• Convenient pre-activated format

• Mild coupling conditions

• High coupling efficiency

• Broad application range

• Multiple packaging options

Epoxy TopAC 6B for Biomolecule Immobilization

Epoxy TopAC 6B is designed for covalent immobilization of suitable organic and biological molecules.

The supplied product information specifically identifies molecules containing:

• Amino groups

• Thiol groups

• Hydroxyl groups

as suitable for chemical coupling to the activated matrix.

Epoxy Resin for Protein and Peptide Immobilization

Proteins and peptides are specifically listed as examples of molecules that can be immobilized using Epoxy TopAC 6B.

The epoxy-activated matrix provides reactive groups for covalent coupling, allowing suitable proteins and peptides to be immobilized onto the agarose support.

Epoxy Resin for Carbohydrate Immobilization

Carbohydrates are also included in the supplied product description as suitable molecules for coupling.

This gives Epoxy TopAC 6B a broader application range for preparing customized immobilized biomolecule and affinity chromatography matrices.

Available Package Sizes

Epoxy TopAC 6B is available in both weight and volume packaging.

Weight packaging:
25 g / 100 g / 500 g / 1 kg / 5 kg / 10 kg

Volume packaging:
25 mL / 100 mL / 300 mL / 1 L / 5 L / 10 L / 20 L

Frequently Asked Questions

What is Epoxy TopAC 6B?

Epoxy TopAC 6B is an epoxy pre-activated affinity chromatography resin based on a 6% agarose matrix.

What is the English product name?

The supplied English name is Epoxy Pre-activated Affinity Resin.

What matrix does Epoxy TopAC 6B use?

According to the supplied information, it uses a 6% agarose matrix.

What is the coupling density?

The specified coupling density is 19–40 μmol epoxide/mL.

Is 19–40 μmol epoxide/mL the binding capacity?

No. The supplied table specifically identifies this value as Coupling Density.

What functional groups can be coupled?

The supplied information specifically lists amino, thiol and hydroxyl groups.

What types of biomolecules can be immobilized?

The supplied product description gives proteins, peptides and carbohydrates as examples.

Can Epoxy TopAC 6B be used for protein immobilization?

Yes. Proteins are specifically included in the supplied product description.

Can it be used for peptide immobilization?

Yes. Peptides are specifically listed as suitable molecules.

Can it be used for carbohydrate immobilization?

Yes. Carbohydrates are also specifically listed.

What are the advantages of Epoxy TopAC 6B?

The supplied information identifies convenient use, mild coupling conditions, high coupling efficiency and a broad application range as advantages.

Is particle size specified?

No. A numerical particle size is not provided in the supplied information.

Is pressure resistance specified?

No. A numerical pressure resistance value is not provided.

What weight packages are available?

Available weight packages are 25 g, 100 g, 500 g, 1 kg, 5 kg and 10 kg.

What volume packages are available?

Available volume packages are 25 mL, 100 mL, 300 mL, 1 L, 5 L, 10 L and 20 L.

What are the SHBC Art.No. numbers?

The corresponding Art.No. are A5187–A5199.

What brand is Epoxy TopAC 6B?

Epoxy TopAC 6B is supplied under the SHBC brand.