Fmoc-Cys(Trt)-Wang Resin CAS103213-32-7 0.3~0.8 Mmol/g, 100~200 Mesh, 1% DVB

Fmoc-Cys(Trt)-Wang Resin for C-Terminal Cysteine Peptides

SHBC Fmoc-Cys(Trt)-Wang Resin is a preloaded solid support for Fmoc solid-phase peptide synthesis of peptide acids containing C-terminal L-cysteine.

The resin contains N-Fmoc-S-trityl-L-cysteine attached to an acid-labile Wang linker. The Trt group protects the cysteine thiol during peptide-chain assembly.

Acidic cleavage releases a C-terminal cysteine peptide acid and removes the Trt group to generate a free thiol.

Product Specifications

Key Advantages of SHBC Fmoc-Cys(Trt)-Wang Resin

Ready-to-Use Preloaded Resin

The C-terminal Fmoc-Cys(Trt) residue is already attached, eliminating the initial amino acid loading step.

Produces C-Terminal Cysteine Peptide Acids

Cleavage from the Wang linker releases a peptide with C-terminal L-cysteine and a free carboxylic acid.

Trt-Protected Thiol

The acid-labile Trt group protects the cysteine sulfur during Fmoc deprotection and coupling cycles.

Free Thiol after Cleavage

Removal of Trt under validated acidic conditions generates a free thiol for oxidation, cyclization, labeling or conjugation.

Flexible Loading Range

The 0.3–0.8 mmol/g loading range supports different peptide lengths, sequence properties and synthesis scales.

Practical Resin Handling

The 1% DVB polystyrene matrix and 100–200 mesh particle size provide useful swelling, reagent access, filtration and washing performance.

How Fmoc-Cys(Trt)-Wang Resin Works

Fmoc-Cys(Trt) is attached to the Wang linker through its carboxyl group. The amino group is protected by Fmoc, while the thiol is protected by Trt.

A general synthesis workflow includes:

1. Pre-swell the resin in a compatible SPPS solvent.

2. Remove the Fmoc protecting group.

3. Couple the next protected amino acid.

4. Repeat Fmoc deprotection and coupling cycles.

5. Wash the resin between reaction steps.

6. Cleave and deprotect the peptide under validated acidic conditions.

7. Purify the peptide and control thiol oxidation as required.

Cleavage, thiol handling and oxidation conditions should be optimized for the target sequence.

Applications

SHBC Fmoc-Cys(Trt)-Wang Resin can be used for:

• Fmoc solid-phase peptide synthesis

• Peptides with C-terminal cysteine

• C-terminal peptide acid preparation

• Disulfide-containing peptide research

• Cyclic peptide development

• Thiol-mediated peptide conjugation

• Site-specific peptide labeling

• Bioconjugation-ready peptides

• Antigenic and epitope peptides

• Enzyme substrate peptides

• Peptide libraries

• Therapeutic peptide research

• Process development and batch synthesis

The free C-terminal cysteine thiol can support controlled disulfide formation or reaction with compatible thiol-selective labels and conjugation reagents.

Choosing the Right Resin Loading

Resin loading influences production capacity, reagent diffusion and aggregation risk.

Lower loading may be preferred for:

• Long peptide sequences

• Hydrophobic peptides

• Aggregation-prone sequences

• Sterically demanding modifications

• Complex disulfide-containing peptides

Higher loading may provide greater batch productivity for shorter and less demanding sequences.

Select the loading according to peptide length, sequence hydrophobicity, synthesis scale and downstream purification requirements.

Comparison with Other Cysteine SPPS Supports

Choose Fmoc-Cys(Trt)-Wang Resin when the required product is a peptide acid ending in L-cysteine.

Product Identification and CAS Clarification

Fmoc-Cys(Trt)-Wang Resin is a polymer-bound product identified by its full product name, loading, particle size, crosslinking and lot documentation.

CAS 103213-32-7 identifies free Fmoc-Cys(Trt)-OH. It is not a unique CAS number for the preloaded Wang resin.

Refer to the SHBC product label, COA and SDS for purchasing and technical identification.

Quality Control and Storage

Important qualification parameters include:

• Fmoc loading or substitution

• Amino acid identity

• Trt protection integrity

• Enantiomeric purity

• Dipeptide content

• Particle-size distribution

• DVB crosslinking

• Moisture content

• Resin swelling performance

• Residual unreacted linker sites

• Test coupling and cleavage performance

• Lot-to-lot consistency

Request the lot-specific SHBC COA for available test results.

Keep the resin tightly sealed in a cool, dry place according to the product label and SDS. Pre-swell the resin completely before synthesis.

After cleavage, protect free-thiol peptides from uncontrolled oxidation when required by the application.

Frequently Asked Questions

What is Fmoc-Cys(Trt)-Wang Resin used for?

It is used in Fmoc SPPS to prepare peptide acids containing C-terminal L-cysteine.

What C-terminus is obtained after cleavage?

The resin releases a peptide with C-terminal L-Cys-COOH.

What does Trt protect?

Trt protects the cysteine thiol group during peptide-chain assembly.

Is the thiol free after cleavage?

Validated acidic cleavage normally removes the Trt group and generates a free cysteine thiol.

Can the peptide be used for conjugation?

The free thiol may be used for disulfide formation, cyclization, labeling or thiol-selective conjugation under validated conditions.

Is the first amino acid already loaded?

Yes. Fmoc-Cys(Trt) is preloaded onto the Wang linker, allowing synthesis to begin with Fmoc removal.

Is CAS 103213-32-7 the CAS number of the resin?

No. CAS 103213-32-7 identifies free Fmoc-Cys(Trt)-OH, not the polymer-bound Wang resin.

What loading should I choose?

Lower loading is commonly considered for long, hydrophobic or aggregation-prone peptides. Higher loading may improve productivity for shorter sequences.

What information is required for a quotation?

Please provide the required quantity, target loading, peptide length, synthesis scale, packaging and required quality documents.

Request a Sample or Bulk Quote

SHBC supplies Fmoc-Cys(Trt)-Wang Resin for Fmoc peptide synthesis, process development and batch production.

Contact Shanghai SanYu Biotechnology Co., Ltd. to request samples, bulk pricing, loading options, packaging information, COA and SDS.

For research and process-development use unless otherwise specified.