Products
SHBC provides colored microspheres, fluorescent microspheres, magnetic beads, silica microspheres, chromatography packing microspheres and biological reagents for diagnostic assay development, nucleic acid extraction, protein purification and separation applications.
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A5106 / A5107 / A5108 / A5109 / A5110 / A5111 / A5112
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Agarose
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Cibacron Blue 3GA
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≥24 mg/mL (Human Serum Albumin)
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25mL 100mL 300mL 1L 5L 10L 20L
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Chromatography Grade
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Blue Sepharose Affinity Resin
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Protein Purification / Human Serum Albumin Purification / Enzyme Purification
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Dye Affinity Chromatography Resin
Product Overview
SHBC HardAC Blue is a dye affinity chromatography resin designed for purification of a broad range of proteins.
The Blue Series is an agarose-based affinity chromatography medium using Cibacron Blue 3GA as the ligand. According to the supplied product information, the medium provides good physical and chemical stability, minimal ligand leaching, long service life and a wide range of applications.
HardAC Blue provides a specified human serum albumin loading of ≥24 mg/mL and is suitable for purification of proteins including dehydrogenases, kinases, transferases, serum albumin, interferons and plasma proteins.
Key Features
Dye affinity chromatography resin
Cibacron Blue 3GA ligand
Agarose-based chromatography medium
Human serum albumin loading ≥24 mg/mL
Broad protein-binding characteristics
Physical and chemical stability
Minimal ligand leaching
Long service life
Suitable for various protein purification applications
Packages from 25 mL to 20 L
Technical Specifications
Parameter | Specification |
|---|---|
Product Name | HardAC Blue |
Brand | SHBC |
English Name | Blue Sepharose Affinity Resin |
Matrix | Agarose |
Ligand | Cibacron Blue 3GA |
Human Serum Albumin Loading | ≥24 mg/mL |
Chromatography Mode | Dye Affinity Chromatography |
Main Application | Protein Purification |
Package | 25 mL / 100 mL / 300 mL / 1 L / 5 L / 10 L / 20 L |
Ordering Information
SHBC Art.No. | Packaging Specification |
|---|---|
A5106 | 25 mL |
A5107 | 100 mL |
A5108 | 300 mL |
A5109 | 1 L |
A5110 | 5 L |
A5111 | 10 L |
A5112 | 20 L |
Cibacron Blue 3GA Affinity Ligand
HardAC Blue uses Cibacron Blue 3GA as its affinity ligand.
According to the supplied Blue Series description, the ligand is incorporated into an agarose-based affinity chromatography medium and can interact with different proteins through both specific and nonspecific mechanisms.
Human Serum Albumin Loading
The specified human serum albumin loading of HardAC Blue is:
≥24 mg/mL
This value is specifically listed in the supplied table as Human Serum Albumin Loading.
It should not be interpreted as the universal binding capacity for all target proteins.
Broad Protein Binding
According to the supplied information, the Blue Series can bind proteins through specific interactions.
It can also interact with proteins through nonspecific:
Charge interactions
Hydrophobic interactions
These characteristics contribute to its broad application in protein purification.
Human Serum Albumin Purification
HardAC Blue provides a specified human serum albumin loading of ≥24 mg/mL.
Serum albumin is specifically listed among the purification applications of the Blue Series, making HardAC Blue suitable for relevant albumin purification workflows.
Enzyme Purification
The supplied Blue Series information identifies several enzyme groups among its applications, including:
Dehydrogenases
Kinases
Transferases
HardAC Blue can therefore be used in suitable affinity chromatography workflows for purification of these types of proteins.
Interferon Purification
Interferons are also specifically included among the applications listed for the Blue Series.
HardAC Blue can be considered for appropriate interferon separation and purification processes.
Plasma Protein Purification
The Blue Series is described as being widely applicable to plasma protein purification.
Its broad interaction mechanisms allow the chromatography medium to be used for purification of selected plasma proteins.
Physical and Chemical Stability
According to the supplied information, the Blue Series provides good physical and chemical stability.
This characteristic supports its use in chromatography purification processes requiring stable affinity media.
No numerical stability ranges are provided in the supplied material.
Minimal Ligand Leaching
The supplied product information states that the ligand is not easily detached from the chromatography medium.
This provides minimal ligand leaching and contributes to the stated long service life of the Blue Series.
Applications
SHBC HardAC Blue can be used for:
Human serum albumin purification
Serum albumin purification
Dehydrogenase purification
Kinase purification
Transferase purification
Interferon purification
Plasma protein purification
General protein purification
Dye affinity chromatography
Product Advantages
HardAC Blue provides:
Cibacron Blue 3GA affinity ligand
Agarose-based chromatography medium
≥24 mg/mL human serum albumin loading
Broad protein-binding characteristics
Specific affinity interactions
Charge interactions
Hydrophobic interactions
Good physical and chemical stability
Minimal ligand leaching
Long service life
Package sizes from 25 mL to 20 L
Dye Affinity Chromatography for Protein Purification
Dye affinity chromatography uses immobilized dye ligands to interact with target proteins.
HardAC Blue uses Cibacron Blue 3GA as its ligand on an agarose-based chromatography medium.
The supplied information states that the medium can interact with proteins through specific interactions as well as nonspecific charge and hydrophobic effects, supporting purification of a broad range of proteins.
HardAC Blue Human Serum Albumin Loading
One of the key specifications differentiating HardAC Blue in the supplied table is its human serum albumin loading:
≥24 mg/mL
This specification can be useful when evaluating a Blue affinity chromatography resin for applications involving serum albumin.
Available Package Sizes
HardAC Blue is available in:
25 mL / 100 mL / 300 mL / 1 L / 5 L / 10 L / 20 L
The package range supports different chromatography processing volumes.
Frequently Asked Questions
What is HardAC Blue?
HardAC Blue is a dye affinity chromatography resin designed for purification of various proteins.
What is the English product name?
The supplied English name is Blue Sepharose Affinity Resin.
What ligand does HardAC Blue use?
It uses Cibacron Blue 3GA as the affinity ligand.
What matrix is used?
The supplied Blue Series information identifies the chromatography medium as agarose-based.
What is the human serum albumin loading?
The specified human serum albumin loading is ≥24 mg/mL.
Is ≥24 mg/mL the binding capacity for all proteins?
No. The supplier table specifically identifies this parameter as Human Serum Albumin Loading.
What proteins can HardAC Blue be used to purify?
The supplied information lists dehydrogenases, kinases, transferases, serum albumin, interferons and plasma proteins.
How does HardAC Blue interact with proteins?
According to the supplied information, protein binding can occur through specific interactions as well as nonspecific charge and hydrophobic interactions.
Does HardAC Blue have good stability?
The Blue Series is described as having good physical and chemical stability.
Does the ligand easily leach?
The supplied information states that the ligand is not easily detached, providing minimal ligand leaching.
Is the particle size specified?
No. A numerical particle size for HardAC Blue is not provided in the supplied table.
Is a pressure resistance value provided?
No. The supplied information does not provide a numerical pressure resistance value.
What package sizes are available?
Available sizes are 25 mL, 100 mL, 300 mL, 1 L, 5 L, 10 L and 20 L.
What are the SHBC Art.No. numbers?
The corresponding Art.No. are A5106–A5112.
What brand is HardAC Blue?
HardAC Blue is supplied under the SHBC brand.