Products
SHBC provides colored microspheres, fluorescent microspheres, magnetic beads, silica microspheres, chromatography packing microspheres and biological reagents for diagnostic assay development, nucleic acid extraction, protein purification and separation applications.
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A5113 / A5114 / A5115 / A5116 / A5117 / A5118 / A5119
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Agarose
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Cibacron Blue 3GA
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≥24 mg/mL (Human Serum Albumin)
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25mL 100mL 300mL 1L 5L 10L 20L
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Chromatography Grade
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Blue Sepharose Affinity Resin F
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Human Serum Albumin Purification / Enzyme Purification / Interferon Purification / Plasma Protein Purification
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Dye Affinity Chromatography Resin
Product Overview
SHBC HardAC Blue F is a dye affinity chromatography resin designed for the purification of a broad range of proteins.
The Blue Series is an agarose-based chromatography medium using Cibacron Blue 3GA as the affinity ligand. The medium can interact with proteins through specific affinity as well as charge and hydrophobic interactions.
HardAC Blue F provides a specified human serum albumin loading of ≥24 mg/mL and is suitable for purification applications involving serum albumin, enzymes, interferons and plasma proteins.
Key Features
Dye affinity chromatography resin
Cibacron Blue 3GA affinity ligand
Agarose-based chromatography medium
≥24 mg/mL human serum albumin loading
Broad protein interaction characteristics
Good physical and chemical stability
Minimal ligand leaching
Long service life
Wide range of protein purification applications
Available from 25 mL to 20 L
Technical Specifications
Parameter | Specification |
|---|---|
Product Name | HardAC Blue F |
Brand | SHBC |
English Name | Blue Sepharose Affinity Resin F |
Series | F |
Matrix | Agarose |
Ligand | Cibacron Blue 3GA |
Human Serum Albumin Loading | ≥24 mg/mL |
Chromatography Mode | Dye Affinity Chromatography |
Main Application | Protein Purification |
Package | 25 mL / 100 mL / 300 mL / 1 L / 5 L / 10 L / 20 L |
Ordering Information
SHBC Art.No. | Packaging Specification |
|---|---|
A5113 | 25 mL |
A5114 | 100 mL |
A5115 | 300 mL |
A5116 | 1 L |
A5117 | 5 L |
A5118 | 10 L |
A5119 | 20 L |
Cibacron Blue 3GA Affinity Ligand
HardAC Blue F uses Cibacron Blue 3GA as the affinity ligand.
According to the supplied Blue Series information, the ligand is immobilized on an agarose-based chromatography medium and provides interactions with a broad range of proteins.
This makes the resin suitable for multiple protein separation and purification applications.
Human Serum Albumin Loading
The specified human serum albumin loading is:
≥24 mg/mL
This specification is specifically referenced to human serum albumin in the supplied product table.
It should not be treated as a universal binding capacity for every protein.
Broad Protein Interaction
According to the supplied product information, Blue Series chromatography media can bind proteins through specific interactions.
Additional nonspecific interactions may include:
Charge interactions
Hydrophobic interactions
These different interaction mechanisms contribute to the broad application range of the Blue affinity chromatography series.
Human Serum Albumin Purification
HardAC Blue F provides ≥24 mg/mL human serum albumin loading.
Serum albumin is specifically identified among the applications of the Blue Series, making HardAC Blue F suitable for relevant albumin separation and purification workflows.
Enzyme Purification
The supplied Blue Series information lists several types of enzymes that can be purified using this chromatography medium, including:
Dehydrogenases
Kinases
Transferases
HardAC Blue F can therefore be used in suitable enzyme purification applications.
Interferon Purification
The supplied product information also identifies interferons among the proteins suitable for purification using the Blue Series.
HardAC Blue F provides another dye affinity chromatography option for suitable interferon purification workflows.
Plasma Protein Purification
HardAC Blue F can also be used for plasma protein purification.
The broad protein interaction characteristics of Cibacron Blue 3GA support affinity chromatography applications involving selected plasma proteins.
Physical and Chemical Stability
The Blue Series is described as providing good physical and chemical stability.
This supports the use of HardAC Blue F in protein chromatography workflows where stable affinity media are required.
No numerical stability range is provided in the supplied information.
Minimal Ligand Leaching
According to the supplied information, the Blue Series ligand is not easily detached.
This provides minimal ligand leaching and contributes to the stated long service life of the chromatography medium.
Applications
SHBC HardAC Blue F can be used for:
Human serum albumin purification
Serum albumin purification
Dehydrogenase purification
Kinase purification
Transferase purification
Interferon purification
Plasma protein purification
General protein purification
Dye affinity chromatography
Protein downstream purification
Product Advantages
HardAC Blue F combines:
Cibacron Blue 3GA ligand
Agarose-based chromatography medium
≥24 mg/mL human serum albumin loading
Broad protein interaction characteristics
Specific affinity interactions
Charge interactions
Hydrophobic interactions
Good physical and chemical stability
Minimal ligand leaching
Long service life
Package sizes up to 20 L
Dye Affinity Chromatography
Dye affinity chromatography uses immobilized dye ligands to interact with target proteins.
HardAC Blue F uses Cibacron Blue 3GA on an agarose-based chromatography medium.
According to the supplied information, proteins can interact with the medium through specific affinity interactions as well as nonspecific charge and hydrophobic interactions.
This enables the Blue Series to be used for purification of different types of proteins.
HardAC Blue F vs. HardAC Blue
According to the supplied table, both products have the same specified human serum albumin loading.
Feature | HardAC Blue F | HardAC Blue |
|---|---|---|
English Name | Blue Sepharose Affinity Resin F | Blue Sepharose Affinity Resin |
Series | F | Standard |
Ligand | Cibacron Blue 3GA | Cibacron Blue 3GA |
Matrix | Agarose | Agarose |
HSA Loading | ≥24 mg/mL | ≥24 mg/mL |
Package Range | 25 mL–20 L | 25 mL–20 L |
The supplied information does not provide enough additional quantitative specifications to make further performance claims about the difference between HardAC Blue F and HardAC Blue.
Blue Series Loading Comparison
Based on the supplied table:
Product | Human Serum Albumin Loading |
|---|---|
TopAC Blue FF | ≥18 mg/mL |
TopAC Blue HP | ≥18 mg/mL |
HardAC Blue | ≥24 mg/mL |
HardAC Blue F | ≥24 mg/mL |
These values specifically refer to human serum albumin loading and should not be interpreted as universal protein binding capacities.
Available Package Sizes
HardAC Blue F is available in:
25 mL / 100 mL / 300 mL / 1 L / 5 L / 10 L / 20 L
Frequently Asked Questions
What is HardAC Blue F?
HardAC Blue F is a dye affinity chromatography resin designed for purification of various proteins.
What is the English product name?
The supplied English name is Blue Sepharose Affinity Resin F.
What ligand does HardAC Blue F use?
It uses Cibacron Blue 3GA as its affinity ligand.
What matrix is used?
The supplied Blue Series information identifies the chromatography medium as agarose-based.
What is the human serum albumin loading?
The specified human serum albumin loading is ≥24 mg/mL.
Is ≥24 mg/mL the binding capacity for every protein?
No. The table specifically identifies this value as Human Serum Albumin Loading.
What proteins can HardAC Blue F be used to purify?
The supplied information lists dehydrogenases, kinases, transferases, serum albumin, interferons and plasma proteins.
How does the resin interact with proteins?
According to the supplied information, interactions include specific affinity as well as nonspecific charge and hydrophobic interactions.
Does the resin have good stability?
Yes. The supplied Blue Series information describes good physical and chemical stability.
Does the ligand easily leach?
The supplied information states that the ligand is not easily detached, providing minimal ligand leaching.
What is the difference between HardAC Blue F and HardAC Blue?
The supplied table identifies them as different product versions, but both have ≥24 mg/mL human serum albumin loading. Additional quantitative differences are not provided.
Is the particle size specified?
No. The supplied table does not provide a numerical particle size.
What package sizes are available?
Available sizes are 25 mL, 100 mL, 300 mL, 1 L, 5 L, 10 L and 20 L.
What are the SHBC Art.No. numbers?
The corresponding Art.No. are A5113–A5119.
What brand is HardAC Blue F?
HardAC Blue F is supplied under the SHBC brand.