Product Overview
SHBC NHS TopAC 4FF is an NHS pre-activated affinity chromatography resin based on the Top-4FF high-flow agarose matrix.
According to the supplied product information, the agarose gel is pre-activated with N-hydroxysuccinimide (NHS) and features spacer arms suitable for coupling small-molecule ligands.
The specified coupling density is 16–23 μmol NHS/mL.
Key Features
NHS pre-activated affinity resin
N-Hydroxysuccinimide (NHS) activation
Top-4FF high-flow agarose matrix
Coupling density: 16–23 μmol NHS/mL
Features spacer arms
Suitable for coupling small-molecule ligands
Designed for affinity media preparation
Weight and volume packaging available
Package sizes from 25 g to 10 kg and 25 mL to 20 L
Technical Specifications
Parameter | Specification |
|---|
Product Name | NHS TopAC 4FF |
Brand | SHBC |
English Name | NHS Pre-activated Affinity Resin |
Series | 4FF |
Matrix | Top-4FF High-Flow Agarose |
Activation | N-Hydroxysuccinimide (NHS) |
Coupling Density | 16–23 μmol NHS/mL |
Spacer Arm | Yes |
Main Application | Small-Molecule Ligand Coupling |
Product Type | Pre-activated Affinity Resin |
Weight Package | 25 g / 100 g / 500 g / 1 kg / 5 kg / 10 kg |
Volume Package | 25 mL / 100 mL / 300 mL / 1 L / 5 L / 10 L / 20 L |
SHBC Art.No. | Packaging Specification |
|---|
A5174 | 25 g |
A5175 | 100 g |
A5176 | 500 g |
A5177 | 1 kg |
A5178 | 5 kg |
A5179 | 10 kg |
A5180 | 25 mL |
A5181 | 100 mL |
A5182 | 300 mL |
A5183 | 1 L |
A5184 | 5 L |
A5185 | 10 L |
A5186 | 20 L |
NHS-Activated Agarose Resin
NHS TopAC 4FF is an affinity chromatography matrix pre-activated with N-hydroxysuccinimide (NHS).
The supplied product information identifies Top-4FF as the agarose matrix used for this product.
The pre-activated format provides a matrix for coupling suitable ligands to prepare customized affinity chromatography media.
Top-4FF High-Flow Agarose Matrix
NHS TopAC 4FF uses the Top-4FF high-flow agarose matrix.
According to the supplied information, NHS TopAC 4FF is one of the NHS-activated agarose gels based on a high-rigidity/high-flow agarose matrix.
No numerical particle size, pressure resistance or recommended flow rate is provided in the supplied table, so these parameters are not specified here.
Coupling Density
The specified coupling density of NHS TopAC 4FF is:
16–23 μmol NHS/mL
The table specifically defines this parameter as Coupling Density (μmol NHS/mL).
Therefore, this specification should not be described as dynamic binding capacity or protein loading capacity.
Spacer Arm Design
The supplied product description states that NHS TopAC 4FF features spacer arms suitable for coupling small-molecule ligands.
The spacer arm separates the coupled ligand from the agarose matrix, making the product suitable for applications involving immobilization of appropriate small-molecule ligands.
Small-Molecule Ligand Coupling
One of the specifically stated applications of NHS TopAC 4FF is the coupling of small-molecule ligands.
The NHS pre-activated matrix and spacer-arm structure provide a ready-to-use support for preparing customized affinity chromatography media with suitable ligands.
Affinity Ligand Immobilization
NHS TopAC 4FF can be used as a pre-activated support for affinity ligand immobilization.
Once a suitable ligand is coupled to the matrix, the resulting affinity medium can be used in appropriate affinity chromatography applications.
The exact ligand compatibility and coupling conditions are not specified in the supplied table.
NHS TopAC 4FF provides a pre-activated agarose support for preparing customized affinity chromatography media.
Its combination of NHS activation, Top-4FF high-flow agarose matrix and spacer-arm design makes it particularly suitable for small-molecule ligand immobilization.
Applications
SHBC NHS TopAC 4FF can be used for:
Small-molecule ligand coupling
Affinity ligand immobilization
Customized affinity media preparation
Affinity chromatography resin preparation
Ligand immobilization
Affinity chromatography research
Product Advantages
NHS TopAC 4FF provides:
NHS pre-activated agarose matrix
Top-4FF high-flow agarose support
16–23 μmol NHS/mL coupling density
Spacer-arm structure
Suitable for small-molecule ligand coupling
Suitable for customized affinity media preparation
Weight and volume packaging options
Scale range from 25 g to 10 kg and 25 mL to 20 L
NHS TopAC 4FF for Small-Molecule Ligands
The supplied product information specifically states that NHS TopAC 4FF has spacer arms suitable for coupling small-molecule ligands.
This makes small-molecule ligand immobilization one of the key positioning points for this pre-activated affinity resin.
NHS Pre-activated Affinity Chromatography Resin
NHS TopAC 4FF combines an NHS pre-activated surface with a Top-4FF high-flow agarose matrix.
Instead of supplying a finished resin with a predetermined affinity ligand, it provides a pre-activated matrix onto which an appropriate ligand can be coupled.
This enables preparation of customized affinity chromatography media for suitable applications.
Available Package Sizes
NHS TopAC 4FF is supplied in both weight and volume packaging.
Weight packaging:
25 g / 100 g / 500 g / 1 kg / 5 kg / 10 kg
Volume packaging:
25 mL / 100 mL / 300 mL / 1 L / 5 L / 10 L / 20 L
Frequently Asked Questions
What is NHS TopAC 4FF?
NHS TopAC 4FF is an NHS pre-activated affinity chromatography resin based on a Top-4FF high-flow agarose matrix.
What is the English product name?
The supplied English name is NHS Pre-activated Affinity Resin.
What does NHS stand for?
In the supplied product description, NHS refers to N-hydroxysuccinimide.
What matrix does NHS TopAC 4FF use?
It uses the Top-4FF high-flow agarose matrix.
What is the coupling density?
The specified coupling density is 16–23 μmol NHS/mL.
Is 16–23 μmol NHS/mL a dynamic binding capacity?
No. The supplied table specifically identifies it as Coupling Density (μmol NHS/mL).
Does NHS TopAC 4FF have a spacer arm?
Yes. The supplied information specifically states that it features spacer arms.
What type of ligands is it suitable for?
The supplied description specifically states that the spacer arms are suitable for coupling small-molecule ligands.
Yes. NHS TopAC 4FF is a pre-activated matrix that can be used for suitable ligand immobilization and customized affinity media preparation.
Is particle size specified?
No. A numerical particle size is not provided in the supplied information.
Is pressure resistance specified?
No. A numerical pressure resistance value is not provided.
Is the recommended flow rate specified?
No. A numerical recommended flow rate is not provided.
What weight packages are available?
The available weight packages are 25 g, 100 g, 500 g, 1 kg, 5 kg and 10 kg.
What volume packages are available?
The available volume packages are 25 mL, 100 mL, 300 mL, 1 L, 5 L, 10 L and 20 L.
What are the SHBC Art.No. numbers?
The corresponding Art.No. are A5174–A5186.
What brand is NHS TopAC 4FF?
NHS TopAC 4FF is supplied under the SHBC brand.
