TopAC Benzamidine 4FF Affinity Chromatography Resin

Product Overview

SHBC TopAC Benzamidine 4FF is an affinity chromatography resin designed for the separation, purification and removal of serine proteases.

According to the supplied product information, TopAC Benzamidine 4FF is prepared by coupling an aminobenzamidine ligand to agarose gel Top-4FF.

Benzamidine derivatives are broad-spectrum inhibitors of serine proteases. This affinity mechanism makes TopAC Benzamidine 4FF suitable for purification of serine proteases or removal of serine proteases from biological samples.

The specified dynamic binding capacity is ≥35 mg/mL for trypsin.

Key Features

• Benzamidine affinity chromatography resin

• Aminobenzamidine affinity ligand

• Agarose gel Top-4FF matrix

• Dynamic binding capacity ≥35 mg/mL (Trypsin)

• Designed for serine protease purification

• Suitable for serine protease removal

• Applicable to biological samples

• Available from 25 mL to 20 L

Technical Specifications

Ordering Information

Aminobenzamidine Affinity Ligand

TopAC Benzamidine 4FF uses aminobenzamidine as the affinity ligand.

According to the supplied information, the aminobenzamidine ligand is coupled to agarose gel Top-4FF to prepare the affinity chromatography medium.

Benzamidine derivatives are described as broad-spectrum inhibitors of serine proteases.

Dynamic Binding Capacity

The specified dynamic binding capacity of TopAC Benzamidine 4FF is:

≥35 mg/mL (Trypsin)

The supplied table specifically uses trypsin as the reference protein for this binding capacity.

Therefore, ≥35 mg/mL should not be interpreted as the binding capacity for every serine protease.

Serine Protease Purification

TopAC Benzamidine 4FF is designed for affinity purification of serine proteases.

The supplied product information specifically lists serine proteases such as:

• Trypsin

• Thrombin

• Urokinase

• Kallikrein

• Prekallikrein

These proteins are included in the supplied description as examples of serine proteases associated with benzamidine affinity chromatography.

Trypsin Purification

Trypsin is specifically used as the reference protein for the dynamic binding capacity of TopAC Benzamidine 4FF.

The specified value is:

≥35 mg Trypsin/mL

This makes trypsin purification one of the key applications identified for the product.

Thrombin and Urokinase Purification

The supplied product information identifies thrombin and urokinase among the serine proteases associated with benzamidine derivatives.

TopAC Benzamidine 4FF can therefore be used in suitable affinity chromatography workflows involving these serine proteases.

Kallikrein and Prekallikrein Purification

Kallikrein and prekallikrein are also specifically listed in the supplied description.

The benzamidine affinity mechanism can be used for appropriate separation and purification workflows involving these serine proteases.

Serine Protease Removal

In addition to purification, TopAC Benzamidine 4FF can be used for removing serine proteases from biological samples.

This is specifically identified as an application in the supplied product information.

The resin can therefore be considered when the process objective is not recovery of the protease itself, but removal of serine protease components from a biological sample.

Applications

SHBC TopAC Benzamidine 4FF can be used for:

• Serine protease purification

• Serine protease removal

• Trypsin purification

• Thrombin purification

• Urokinase purification

• Kallikrein purification

• Prekallikrein purification

• Protease separation

• Biological sample processing

• Affinity chromatography

Product Advantages

TopAC Benzamidine 4FF provides:

• Aminobenzamidine affinity ligand

• Agarose gel Top-4FF matrix

• ≥35 mg/mL dynamic binding capacity for trypsin

• Specific application for serine proteases

• Suitable for protease purification

• Suitable for protease removal

• Broad application to different serine proteases

• Seven package sizes from 25 mL to 20 L

Benzamidine Affinity Chromatography

Benzamidine derivatives are described in the supplied product information as broad-spectrum inhibitors of serine proteases.

TopAC Benzamidine 4FF utilizes this interaction by coupling an aminobenzamidine ligand to agarose gel Top-4FF.

The resulting affinity chromatography resin can be used to separate and purify serine proteases or remove them from biological samples.

TopAC Benzamidine 4FF for Trypsin

Trypsin is one of the key target proteins for TopAC Benzamidine 4FF.

The supplied specification gives a dynamic binding capacity of:

≥35 mg/mL (Trypsin)

This value provides a defined reference specification when evaluating the resin for trypsin-related affinity purification applications.

Available Package Sizes

TopAC Benzamidine 4FF is available in:

25 mL / 100 mL / 300 mL / 1 L / 5 L / 10 L / 20 L

Frequently Asked Questions

What is TopAC Benzamidine 4FF?

TopAC Benzamidine 4FF is an affinity chromatography resin for purification or removal of serine proteases.

What is the English product name?

The supplied English name is Benzamidine Affinity Chromatography Resin 4FF.

What ligand does TopAC Benzamidine 4FF use?

The supplied information states that it uses an aminobenzamidine ligand.

What matrix is used?

The aminobenzamidine ligand is coupled to agarose gel Top-4FF.

What is the dynamic binding capacity?

The specified dynamic binding capacity is ≥35 mg/mL for trypsin.

Does ≥35 mg/mL apply to all serine proteases?

No. The supplied table specifically gives this value using trypsin as the reference protein.

What is the main application?

The main applications are serine protease purification and serine protease removal.

What serine proteases are listed in the supplied information?

The supplied information lists trypsin, thrombin, urokinase, kallikrein and prekallikrein.

Can TopAC Benzamidine 4FF be used to remove proteases?

Yes. The supplied description specifically states that the medium can be used for removing serine proteases from biological samples.

Can it be used for trypsin purification?

Yes. Trypsin is specifically identified, and the product has a stated dynamic binding capacity of ≥35 mg/mL for trypsin.

Is the particle size specified?

No. The supplied table does not provide a numerical particle size.

Is the recommended flow rate specified?

No. A numerical recommended flow rate is not provided in the supplied information.

Is pressure resistance specified?

No. A numerical pressure resistance value is not provided.

What package sizes are available?

Available sizes are 25 mL, 100 mL, 300 mL, 1 L, 5 L, 10 L and 20 L.

What are the SHBC Art.No. numbers?

The corresponding Art.No. are A5134–A5140.

What brand is TopAC Benzamidine 4FF?

TopAC Benzamidine 4FF is supplied under the SHBC brand.