TopAC Blue FF Dye Affinity Chromatography Resin

Product Overview

SHBC TopAC Blue FF is a dye affinity chromatography resin designed for purification of a broad range of proteins.

According to the supplied Blue Series information, the chromatography medium is based on agarose with Cibacron Blue 3GA as the ligand.

TopAC Blue FF provides a specified human serum albumin loading of ≥18 mg/mL and can be used for purification of proteins including dehydrogenases, kinases, transferases, serum albumin, interferons and plasma proteins.

Key Features

• Dye affinity chromatography resin

• Agarose-based chromatography matrix

• Cibacron Blue 3GA ligand

• Human serum albumin loading: ≥18 mg/mL

• Broad protein-binding characteristics

• Good physical and chemical stability

• Minimal ligand leaching

• Long service life

• Wide range of applications

• Available from 25 mL to 20 L

Technical Specifications

Ordering Information

Cibacron Blue 3GA Affinity Ligand

TopAC Blue FF uses Cibacron Blue 3GA as its affinity ligand.

According to the supplied series description, Cibacron Blue 3GA is immobilized on an agarose-based matrix to form the Blue Series affinity chromatography medium.

The ligand enables interaction with different proteins, providing a broad application range for protein separation and purification.

Human Serum Albumin Loading

The specified human serum albumin loading of TopAC Blue FF is:

≥18 mg/mL

This value is specifically identified in the supplied product table as Human Serum Albumin Loading.

It should therefore not be generalized as the binding capacity for every type of protein.

Protein Binding Mechanism

According to the supplied information, the Blue Series can bind proteins through specific interactions.

It can also interact with proteins through nonspecific mechanisms involving:

• Charge interactions

• Hydrophobic effects

These multiple interaction mechanisms contribute to the broad protein purification applications of the Blue Series.

Human Serum Albumin Purification

TopAC Blue FF has a specified human serum albumin loading of ≥18 mg/mL, making serum albumin one of the protein targets supported by the supplied product information.

The Blue Series is specifically described as being widely used in serum albumin purification.

Enzyme Purification

The supplied product information lists several enzyme classes among the applications of the Blue Series.

TopAC Blue FF can therefore be used in suitable purification workflows involving:

• Dehydrogenases

• Kinases

• Transferases

Plasma Protein Purification

The Blue Series is also described as being applicable to plasma protein purification.

Its broad protein-binding characteristics make TopAC Blue FF suitable for chromatography workflows involving selected plasma proteins.

Interferon Purification

Interferons are specifically included among the proteins listed in the supplied Blue Series application information.

TopAC Blue FF can therefore be considered for suitable affinity chromatography purification workflows involving interferon proteins.

Physical and Chemical Stability

The supplied Blue Series description states that the chromatography medium has good physical and chemical stability.

This characteristic supports its use in protein purification chromatography applications.

No numerical chemical-resistance or pressure-resistance values are provided in the supplied information, so no additional values are added.

Minimal Ligand Leaching

The Blue Series is described as having minimal ligand leaching.

This characteristic, together with its physical and chemical stability, contributes to the stated long service life of the chromatography medium.

Applications

SHBC TopAC Blue FF can be used for:

• Human serum albumin purification

• Serum albumin purification

• Dehydrogenase purification

• Kinase purification

• Transferase purification

• Interferon purification

• Plasma protein purification

• General protein purification

• Dye affinity chromatography

Product Advantages

TopAC Blue FF provides:

• Cibacron Blue 3GA affinity ligand

• Agarose-based matrix

• ≥18 mg/mL human serum albumin loading

• Specific and nonspecific protein interactions

• Broad protein purification applications

• Good physical stability

• Good chemical stability

• Minimal ligand leaching

• Long service life

• Package sizes from 25 mL to 20 L

Dye Affinity Chromatography

Dye affinity chromatography uses immobilized dye ligands to interact with target proteins.

For TopAC Blue FF, Cibacron Blue 3GA is used as the ligand on an agarose-based chromatography matrix.

According to the supplied product information, the medium can bind proteins through both specific affinity interactions and nonspecific charge and hydrophobic interactions.

This provides a broad range of potential applications in protein purification.

Available Package Sizes

TopAC Blue FF is available in:

25 mL / 100 mL / 300 mL / 1 L / 5 L / 10 L / 20 L

These package options support different protein purification scales.

Frequently Asked Questions

What is TopAC Blue FF?

TopAC Blue FF is a dye affinity chromatography resin designed for purification of various proteins.

What is the English product name?

The supplied English name is Blue Sepharose Affinity Resin FF.

What ligand does TopAC Blue FF use?

It uses Cibacron Blue 3GA as the affinity ligand.

What matrix is used?

According to the supplied Blue Series information, the chromatography medium is based on agarose.

What is the human serum albumin loading?

The specified human serum albumin loading is ≥18 mg/mL.

Is ≥18 mg/mL the binding capacity for every protein?

No. The table specifically identifies this value as Human Serum Albumin Loading, so it should not be presented as a universal protein binding capacity.

What proteins can TopAC Blue FF be used to purify?

The supplied information lists dehydrogenases, kinases, transferases, serum albumin, interferons and plasma proteins.

How does the Blue Series interact with proteins?

The supplied information states that it can bind proteins through specific interactions and through nonspecific charge and hydrophobic interactions.

Does TopAC Blue FF have good chemical stability?

Yes. The Blue Series is described as having good physical and chemical stability.

Does the ligand easily leach from the resin?

The supplied information describes the Blue Series as having minimal ligand leaching.

Is the particle size specified?

No. A numerical particle size for TopAC Blue FF is not provided in the supplied table.

What package sizes are available?

Available sizes are 25 mL, 100 mL, 300 mL, 1 L, 5 L, 10 L and 20 L.

What are the SHBC Art.No. numbers?

The corresponding Art.No. are A5092–A5098.

What brand is TopAC Blue FF?

TopAC Blue FF is supplied under the SHBC brand.