TopAC Blue HP Dye Affinity Chromatography Resin

Product Overview

SHBC TopAC Blue HP is a dye affinity chromatography resin for purification of various proteins.

The Blue Series is based on agarose with Cibacron Blue 3GA as the ligand. It provides physical and chemical stability, minimal ligand leaching, long service life and a broad range of protein purification applications.

TopAC Blue HP has a specified human serum albumin loading of ≥18 mg/mL and can be used for purification of proteins including serum albumin, dehydrogenases, kinases, transferases, interferons and plasma proteins.

Key Features

• Dye affinity chromatography resin

• Cibacron Blue 3GA ligand

• Agarose-based matrix

• Human serum albumin loading ≥18 mg/mL

• Broad protein-binding characteristics

• Physical and chemical stability

• Minimal ligand leaching

• Long service life

• Suitable for various protein purification applications

• Package sizes from 25 mL to 20 L

Technical Specifications

Ordering Information

Cibacron Blue 3GA Ligand

TopAC Blue HP uses Cibacron Blue 3GA as its affinity ligand.

The supplied Blue Series information describes Cibacron Blue 3GA as the ligand immobilized on an agarose-based chromatography medium.

This affinity ligand enables interaction with a broad range of proteins.

Human Serum Albumin Loading

The specified human serum albumin loading is:

≥18 mg/mL

This parameter is specifically listed as Human Serum Albumin Loading in the supplied table.

It should not be interpreted as a universal binding capacity for all proteins.

Protein Binding Characteristics

According to the supplied Blue Series description, this chromatography medium can bind proteins through specific interactions.

Proteins may also interact with the medium through nonspecific mechanisms involving:

• Charge interactions

• Hydrophobic interactions

These characteristics contribute to the broad application range of the Blue Series.

Human Serum Albumin Purification

TopAC Blue HP provides a specified human serum albumin loading of ≥18 mg/mL.

Serum albumin is specifically listed among the purification applications of the Blue Series, making TopAC Blue HP suitable for relevant albumin purification workflows.

Enzyme Purification

The Blue Series is widely applicable to purification of different enzymes.

The supplied information specifically lists:

• Dehydrogenases

• Kinases

• Transferases

TopAC Blue HP can therefore be used in suitable enzyme separation and purification workflows.

Interferon and Plasma Protein Purification

In addition to enzymes and serum albumin, the supplied product information lists interferons and plasma proteins among the applications of the Blue Series.

This broad protein interaction profile makes TopAC Blue HP suitable for a variety of affinity chromatography applications.

Physical and Chemical Stability

The Blue Series is described as having good physical and chemical stability.

It also features minimal ligand leaching and a long service life, supporting repeated chromatography purification applications where appropriate.

Specific numerical stability ranges are not provided in the supplied information.

Minimal Ligand Leaching

The supplied product description states that the ligand is not easily detached, providing minimal ligand leaching.

Together with the stated physical and chemical stability, this contributes to the long service life of the Blue Series chromatography medium.

Applications

SHBC TopAC Blue HP can be used for:

• Human serum albumin purification

• Serum albumin purification

• Dehydrogenase purification

• Kinase purification

• Transferase purification

• Interferon purification

• Plasma protein purification

• General protein purification

• Dye affinity chromatography

Product Advantages

TopAC Blue HP provides:

• Cibacron Blue 3GA affinity ligand

• Agarose-based matrix

• ≥18 mg/mL human serum albumin loading

• Broad protein-binding characteristics

• Specific protein interactions

• Charge-based interactions

• Hydrophobic interactions

• Good physical and chemical stability

• Minimal ligand leaching

• Long service life

• Package sizes from 25 mL to 20 L

Dye Affinity Chromatography for Protein Purification

Dye affinity chromatography uses immobilized dye ligands to interact with proteins.

TopAC Blue HP uses Cibacron Blue 3GA on an agarose-based chromatography medium.

According to the supplied information, proteins can interact with the medium through both specific affinity and nonspecific charge or hydrophobic interactions. This gives the Blue Series a broad range of protein purification applications.

Available Package Sizes

TopAC Blue HP is available in:

25 mL / 100 mL / 300 mL / 1 L / 5 L / 10 L / 20 L

The range supports different chromatography purification volumes.

Frequently Asked Questions

What is TopAC Blue HP?

TopAC Blue HP is a dye affinity chromatography resin for purification of various proteins.

What is the English product name?

The supplied English name is Blue Sepharose Affinity Resin HP.

What ligand is used?

TopAC Blue HP uses Cibacron Blue 3GA as the affinity ligand.

What matrix does it use?

The Blue Series is based on agarose.

What is the human serum albumin loading?

The specified human serum albumin loading is ≥18 mg/mL.

Is ≥18 mg/mL a universal protein binding capacity?

No. The supplied table specifically identifies this value as Human Serum Albumin Loading.

What proteins can TopAC Blue HP be used to purify?

The supplied information lists dehydrogenases, kinases, transferases, serum albumin, interferons and plasma proteins.

How does the resin interact with proteins?

According to the supplied information, it can bind proteins through specific interactions as well as nonspecific charge and hydrophobic interactions.

Does it have good physical and chemical stability?

Yes. Good physical and chemical stability is specifically stated in the supplied Blue Series description.

Does the ligand easily leach?

The supplied information states that the ligand is not easily detached, giving the medium minimal ligand leaching.

Is the particle size specified?

No. A numerical particle size for TopAC Blue HP is not provided in the supplied table.

Is a pressure-resistance value specified?

No. The supplied information does not provide a numerical pressure-resistance value for TopAC Blue HP.

What package sizes are available?

Available sizes are 25 mL, 100 mL, 300 mL, 1 L, 5 L, 10 L and 20 L.

What are the SHBC Art.No. numbers?

The corresponding Art.No. are A5099–A5105.

What brand is TopAC Blue HP?

TopAC Blue HP is supplied under the SHBC brand.