TopAC Heparin FF Affinity Chromatography Resin

Product Overview

SHBC TopAC Heparin FF is a heparin affinity chromatography resin designed for separation and purification of a broad range of biomolecules.

The Heparin Series is prepared by covalently coupling heparin to highly cross-linked agarose, providing an affinity chromatography medium with broad biomolecule-binding characteristics.

TopAC Heparin FF has a specified ligand density of approximately 4 mg Heparin/mL and can be used for purification of various enzymes, coagulation-related proteins, growth factors and other heparin-binding biomolecules.

Key Features

• Heparin affinity chromatography resin

• Heparin affinity ligand

• Highly cross-linked agarose matrix

• Ligand density ≈4 mg Heparin/mL

• Suitable for a broad range of biomolecules

• Physical and chemical stability

• Non-leaching ligand characteristics

• Long service life

• Wide application range

• Easy scale-up

• Available from 25 mL to 20 L

Technical Specifications

Ordering Information

Heparin Affinity Ligand

TopAC Heparin FF uses heparin as the affinity ligand.

According to the supplied product information, heparin is covalently coupled to highly cross-linked agarose to form the affinity chromatography medium.

The specified ligand density for TopAC Heparin FF is:

≈4 mg Heparin/mL

Broad Biomolecule Purification

The Heparin Series is designed for separation and purification of various biomolecules.

According to the supplied product information, applications include different types of:

• Enzymes

• Coagulation factors

• Lipases

• Collagenases

• DNA polymerases

• Growth factors

• Other heparin-binding biomolecules

This broad binding range makes TopAC Heparin FF suitable for different affinity chromatography workflows.

Enzyme Purification

The supplied Heparin Series information specifically identifies various enzymes among its purification applications.

Examples include:

• Antithrombin III

• Thrombin-like enzymes

• Lipoprotein lipase

• Collagenase

• DNA polymerase

TopAC Heparin FF can therefore be used for suitable enzyme and protein purification applications.

Coagulation Factor Purification

The Heparin Series can be applied to purification of coagulation-related biomolecules.

The supplied information specifically lists:

• Human coagulation factor IX

• Human coagulation factor XI

• Human coagulation factor VIII

These applications make heparin affinity chromatography useful for suitable separation and purification workflows involving coagulation proteins.

Growth Factor Purification

A major application area of the Heparin Series is the purification of different growth factors.

The supplied product information lists:

• Human interleukins

• Human prostate growth factors

• Recombinant human vascular endothelial growth factor

• Chondrocyte growth factors

• Basic fibroblast growth factors

• Recombinant human acidic fibroblast growth factors

• Recombinant hepatocyte growth factors

• Recombinant human keratinocyte growth factors

TopAC Heparin FF can therefore be used in suitable growth factor purification processes.

Other Heparin-Binding Biomolecules

The supplied information also identifies additional biomolecules that can interact with Heparin Series chromatography media, including:

• Recombinant mouse heparin cofactor II

• Recombinant human platelet factor IV

• Recombinant human endostatin

This supports the use of TopAC Heparin FF across a broad range of biomolecule purification applications.

Physical and Chemical Stability

According to the supplied Heparin Series information, the chromatography medium provides good physical and chemical stability.

This characteristic supports its use in biomolecule purification processes requiring stable affinity chromatography media.

Specific numerical stability ranges are not provided in the supplied table.

Non-Leaching Ligand

The supplied product description states that the Heparin Series provides non-leaching ligand characteristics.

Stable ligand attachment helps support reliable affinity chromatography performance and contributes to the stated long service life of the medium.

Long Service Life and Scale-Up

The Heparin Series is described as having:

• Long service life

• Wide application range

• Easy scalability

TopAC Heparin FF is available in package sizes ranging from 25 mL to 20 L, supporting different chromatography processing volumes.

Applications

SHBC TopAC Heparin FF can be used for:

• Heparin affinity chromatography

• Enzyme purification

• Antithrombin III purification

• Thrombin-like enzyme purification

• Coagulation factor purification

• Lipoprotein lipase purification

• Collagenase purification

• DNA polymerase purification

• Growth factor purification

• Interleukin purification

• Vascular endothelial growth factor purification

• Fibroblast growth factor purification

• Hepatocyte growth factor purification

• Keratinocyte growth factor purification

• General heparin-binding biomolecule purification

Product Advantages

TopAC Heparin FF combines:

• Highly cross-linked agarose matrix

• Heparin affinity ligand

• ≈4 mg Heparin/mL ligand density

• Broad biomolecule affinity

• Physical and chemical stability

• Non-leaching ligand characteristics

• Long service life

• Wide application range

• Easy scale-up

• Package sizes from 25 mL to 20 L

Heparin Affinity Chromatography

Heparin affinity chromatography uses immobilized heparin as an affinity ligand for separation and purification of biomolecules.

TopAC Heparin FF is prepared by covalently coupling heparin to highly cross-linked agarose.

The resulting affinity medium can be used for purification of various enzymes, coagulation factors, growth factors and other biomolecules listed in the supplied Heparin Series information.

Available Package Sizes

TopAC Heparin FF is available in:

25 mL / 100 mL / 300 mL / 1 L / 5 L / 10 L / 20 L

This range supports both smaller purification requirements and larger-scale chromatography processes.

Frequently Asked Questions

What is TopAC Heparin FF?

TopAC Heparin FF is a heparin affinity chromatography resin for separation and purification of various biomolecules.

What is the English product name?

The supplied English name is Heparin Affinity Chromatography Resin FF.

What ligand does TopAC Heparin FF use?

It uses heparin as the affinity ligand.

What matrix is used?

According to the supplied product information, heparin is covalently coupled to highly cross-linked agarose.

What is the ligand density?

The specified ligand density is approximately 4 mg Heparin/mL.

Is 4 mg/mL the protein binding capacity?

No. The supplied table specifically identifies ≈4 mg Heparin/mL as Ligand Density, not protein binding capacity.

What biomolecules can TopAC Heparin FF be used to purify?

Applications listed in the supplied information include enzymes, coagulation factors, growth factors, DNA polymerase and other heparin-binding biomolecules.

Can it be used for coagulation factor purification?

Yes. The supplied information specifically lists human coagulation factors IX, XI and VIII.

Can it be used for growth factor purification?

Yes. Multiple growth factors are listed, including vascular endothelial, fibroblast, hepatocyte and keratinocyte growth factors.

Does the resin have good stability?

Yes. The supplied Heparin Series information states that the medium has good physical and chemical stability.

Is the ligand easily leached?

The supplied information describes the ligand as non-leaching.

Is the particle size specified?

No. A numerical particle size for TopAC Heparin FF is not provided in the supplied table.

What package sizes are available?

Available package sizes are 25 mL, 100 mL, 300 mL, 1 L, 5 L, 10 L and 20 L.

What are the SHBC Art.No. numbers?

The corresponding Art.No. are A5120–A5126.

What brand is TopAC Heparin FF?

TopAC Heparin FF is supplied under the SHBC brand.