TopAC Heparin HP Affinity Chromatography Resin

Product Overview

SHBC TopAC Heparin HP is a heparin affinity chromatography resin designed for separation and purification of various biomolecules.

The Heparin Series is prepared by covalently coupling heparin to highly cross-linked agarose. This provides an affinity chromatography medium suitable for purification of various enzymes, coagulation factors, growth factors and other heparin-binding biomolecules.

TopAC Heparin HP provides a specified ligand density of approximately 10 mg Heparin/mL.

Key Features

• Heparin affinity chromatography resin

• Heparin affinity ligand

• Highly cross-linked agarose matrix

• Ligand density ≈10 mg Heparin/mL

• Broad biomolecule purification applications

• Good physical and chemical stability

• Non-leaching ligand characteristics

• Long service life

• Wide application range

• Easy scalability

• Package sizes from 25 mL to 20 L

Technical Specifications

Ordering Information

Heparin Affinity Ligand

TopAC Heparin HP uses heparin as the affinity ligand.

According to the supplied Heparin Series information, heparin is covalently coupled to a highly cross-linked agarose matrix.

The specified ligand density of TopAC Heparin HP is:

≈10 mg Heparin/mL

This value represents ligand density, not the dynamic binding capacity of a target protein.

Biomolecule Purification

The Heparin Series has a broad application range for the separation and purification of biomolecules.

The supplied information identifies applications involving:

• Enzymes

• Coagulation factors

• Lipoprotein lipase

• Collagenase

• DNA polymerase

• Growth factors

• Other heparin-binding biomolecules

Enzyme Purification

The Heparin Series can be used for purification of various enzymes and enzyme-related proteins.

The supplied information specifically lists:

• Antithrombin III

• Thrombin-like enzymes

• Lipoprotein lipase

• Collagenase

• DNA polymerase

TopAC Heparin HP can therefore be used in suitable affinity chromatography workflows involving these biomolecules.

Coagulation Factor Purification

Heparin affinity chromatography can also be applied to purification of coagulation-related proteins.

The supplied product information specifically lists:

• Human coagulation factor IX

• Human coagulation factor XI

• Human coagulation factor VIII

TopAC Heparin HP can be used for appropriate purification workflows involving these coagulation factors.

Growth Factor Purification

The supplied Heparin Series information lists a wide range of growth factors among its applications, including:

• Human interleukins

• Human prostate growth factors

• Recombinant human vascular endothelial growth factor

• Chondrocyte growth factors

• Basic fibroblast growth factors

• Recombinant human acidic fibroblast growth factors

• Recombinant hepatocyte growth factors

• Recombinant human keratinocyte growth factors

This makes TopAC Heparin HP suitable for various growth factor purification applications.

Other Heparin-Binding Biomolecules

Additional biomolecules listed in the supplied product information include:

• Recombinant mouse heparin cofactor II

• Recombinant human platelet factor IV

• Recombinant human endostatin

These applications demonstrate the broad biomolecule affinity described for the Heparin Series.

Physical and Chemical Stability

The supplied information describes the Heparin Series as having good physical and chemical stability.

This characteristic supports use in affinity chromatography purification processes requiring stable chromatography media.

No numerical stability range is provided in the supplied information.

Non-Leaching Ligand Characteristics

According to the supplied Heparin Series description, the chromatography medium has non-leaching ligand characteristics.

Stable ligand attachment contributes to the stated long service life of the chromatography resin.

Long Service Life and Scalability

The Heparin Series is described as providing:

• Long service life

• Wide application range

• Easy scalability

TopAC Heparin HP is available in packages ranging from 25 mL to 20 L, supporting different purification volumes.

Applications

SHBC TopAC Heparin HP can be used for:

• Heparin affinity chromatography

• Enzyme purification

• Antithrombin III purification

• Thrombin-like enzyme purification

• Coagulation factor purification

• Lipoprotein lipase purification

• Collagenase purification

• DNA polymerase purification

• Growth factor purification

• Interleukin purification

• Vascular endothelial growth factor purification

• Fibroblast growth factor purification

• Hepatocyte growth factor purification

• Keratinocyte growth factor purification

• General heparin-binding biomolecule purification

Product Advantages

TopAC Heparin HP combines:

• Highly cross-linked agarose matrix

• Heparin affinity ligand

• ≈10 mg Heparin/mL ligand density

• Broad biomolecule affinity

• Good physical and chemical stability

• Non-leaching ligand characteristics

• Long service life

• Wide application range

• Easy scalability

• Packages from 25 mL to 20 L

Heparin Affinity Chromatography

Heparin affinity chromatography uses immobilized heparin to interact with biomolecules that have affinity for the ligand.

TopAC Heparin HP is prepared by covalently coupling heparin to highly cross-linked agarose.

The supplied Heparin Series information describes applications involving enzymes, coagulation factors, growth factors and other biomolecules.

TopAC Heparin HP vs. TopAC Heparin FF

Based strictly on the supplied table, the primary quantitative difference provided between these two products is their heparin ligand density.

The supplied information does not provide enough numerical data to claim differences in flow rate, resolution, pressure resistance or binding capacity between HP and FF.

Available Package Sizes

TopAC Heparin HP is available in:

25 mL / 100 mL / 300 mL / 1 L / 5 L / 10 L / 20 L

Frequently Asked Questions

What is TopAC Heparin HP?

TopAC Heparin HP is a heparin affinity chromatography resin for purification of various biomolecules.

What is the English product name?

The supplied English name is Heparin Affinity Chromatography Resin HP.

What ligand does TopAC Heparin HP use?

It uses heparin as the affinity ligand.

What matrix is used?

The supplied information states that heparin is covalently coupled to highly cross-linked agarose.

What is the ligand density?

The specified ligand density is approximately 10 mg Heparin/mL.

Is ≈10 mg/mL the protein binding capacity?

No. The supplied table specifically identifies this value as Ligand Density (mg Heparin/mL).

What biomolecules can TopAC Heparin HP be used to purify?

The supplied information lists enzymes, coagulation factors, growth factors and other heparin-binding biomolecules.

Can it be used for coagulation factor purification?

Yes. The supplied information specifically lists human coagulation factors IX, XI and VIII.

Can it be used for growth factor purification?

Yes. The supplied information lists multiple growth factors, including vascular endothelial, fibroblast, hepatocyte and keratinocyte growth factors.

What is the difference between TopAC Heparin HP and FF?

The supplied table specifies approximately 10 mg Heparin/mL ligand density for HP and approximately 4 mg Heparin/mL for FF. Other performance differences are not quantified in the supplied information.

Is the particle size specified?

No. A numerical particle size is not provided in the supplied table.

Is the recommended flow rate specified?

No. The supplied information does not provide a numerical recommended flow rate.

What package sizes are available?

Available sizes are 25 mL, 100 mL, 300 mL, 1 L, 5 L, 10 L and 20 L.

What are the SHBC Art.No. numbers?

The corresponding Art.No. are A5127–A5133.

What brand is TopAC Heparin HP?

TopAC Heparin HP is supplied under the SHBC brand.