300nm Carboxyl Green-Fluo Microspheres CFG300-10 1%

300nm Carboxyl Green-Fluo Microspheres CFG300-10

SHBC CFG300-10 are 300 nm carboxyl-functionalized green fluorescent microspheres developed for lateral flow assays, fluorescent immunoassays, biosensors and biomolecule conjugation.

The particles feature 470/488 nm excitation, 525 nm emission and 1% solid content. Reactive surface carboxyl groups support covalent coupling with antibodies, antigens, proteins and peptides.

Product Specifications

Batch-specific particle size, size distribution, fluorescence intensity and carboxyl content are available in the Certificate of Analysis.

Green Fluorescence at 470/488–525nm

CFG300-10 can be excited using approximately 470 nm or 488 nm light and emits green fluorescence near 525 nm.

The optical profile is suitable for compatible fluorescence readers, imaging systems and green-channel detection instruments.

Key Advantages

Bright Green Fluorescence

The 525 nm emission provides a clear signal for qualitative and quantitative fluorescence detection.

Uniform 300nm Particle Size

Controlled particle sizing supports repeatable membrane migration, biomolecule coupling and assay performance.

Reactive COOH Surface

Surface carboxyl groups enable covalent immobilization of antibodies, antigens, proteins and other amino-containing molecules.

High Signal Capacity

The 300 nm particle size provides strong accumulated fluorescence at the test or detection area.

Efficient Biomolecule Loading

The particle surface provides suitable coupling area for antibodies, proteins, peptides and enzymes.

R&D and Bulk Supply

SHBC supports samples, pilot validation, customized specifications and industrial batch production.

Main Applications

Fluorescent Lateral Flow Assays

CFG300-10 can be used as a fluorescent reporter particle in qualitative and quantitative LFIA development.

Fluorescence Immunoassays

Suitable for sandwich, competitive and indirect fluorescent immunoassay formats.

Antibody and Antigen Conjugation

The carboxyl surface supports covalent immobilization of antibodies, antigens and recombinant proteins.

Biosensor Development

Can be used as a fluorescent signal carrier in optical biosensors and surface-based detection systems.

Protein and Peptide Labeling

Suitable for coupling proteins, enzymes, peptides and other amino-containing biomolecules.

Microfluidic Detection

Can be evaluated in compatible microfluidic channels, biochips and fluorescence detection platforms.

Diagnostic Reagent Production

Suitable for medical, veterinary, food-safety and environmental assay development.

Benefits for Fluorescent Immunoassays

CFG300-10 provides:

• Excitation compatible with 470/488 nm

• Green emission near 525 nm

• Reactive carboxyl coupling sites

• Strong accumulated fluorescence

• Good biomolecule-loading capacity

• Suitable membrane-assay performance

• Flexible surface modification

• Custom and bulk manufacturing

Antibody and Protein Coupling

Surface carboxyl groups can be activated using EDC or EDC/NHS chemistry and coupled with primary amine groups on antibodies or proteins.

A general coupling workflow includes:

1. Redisperse and wash the microspheres.

2. Activate the surface carboxyl groups.

3. Add the antibody or target protein.

4. Block remaining reactive sites.

5. Wash and resuspend the conjugate.

6. Evaluate coupling and fluorescence performance.

Coupling pH, activation time, reagent ratio and protein concentration should be optimized for each biomolecule.

Avoid primary amine-containing buffers during the active coupling step because they may compete with the target protein.

LFIA Development Considerations

For lateral flow assay development, optimize:

• Antibody-to-particle ratio

• Microsphere concentration

• Nitrocellulose membrane type

• Conjugate pad release

• Running buffer composition

• Surfactant concentration

• Test-line capture density

• Membrane migration rate

• Background fluorescence

• Reader filter settings

The final formulation should balance fluorescence intensity, migration speed and low non-specific background.

Why Use 300nm Fluorescent Microspheres?

Compared with smaller fluorescent nanoparticles, 300 nm microspheres may provide:

• Higher fluorescence per particle

• Greater ligand-loading area

• Stronger test-line accumulation

• Easier particle recovery

• Improved optical detection

• Good suitability for quantitative LFIA

Actual assay sensitivity should be confirmed using the selected antibody pair, membrane and fluorescence reader.

Dispersion and Handling

Before use:

• Mix gently before sampling

• Redisperse settled particles completely

• Use controlled bath sonication when required

• Avoid excessive bubbles and foam

• Protect the suspension from strong light

• Prevent the particles from drying

• Avoid repeated freeze-thaw cycles

• Confirm buffer compatibility

High salt concentration, unsuitable solvents or incorrect pH may reduce dispersion stability.

Purification and Washing

Suitable purification methods may include:

• Centrifugation

• Ultrafiltration

• Tangential flow filtration

• Size-exclusion chromatography

• Dialysis

Centrifugation speed and time should be optimized to achieve efficient recovery without producing a difficult-to-disperse pellet.

Assay Optimization

Important development parameters include:

• Carboxyl group density

• Antibody loading

• Coupling efficiency

• Blocking reagent

• Conjugate stability

• Particle aggregation

• Membrane migration

• Test-line intensity

• Non-specific adsorption

• Batch consistency

Small-scale screening is recommended before production scale-up.

Instrument Compatibility

Use fluorescence detection systems compatible with:

• Excitation near 470 or 488 nm

• Emission detection near 525 nm

• Suitable green-channel filters

• Appropriate detector sensitivity

Reader calibration and signal range should be confirmed during assay development.

Custom Carboxyl Green-Fluo Microspheres

SHBC can customize fluorescent microspheres according to assay and production requirements.

Customization options may include:

• Custom particle size

• Custom excitation and emission wavelength

• Different fluorescence colors

• Carboxyl group density

• Fluorescence intensity

• Solid content

• Dispersion buffer

• Biomolecule conjugation

• Custom packaging

• OEM and ODM production

Please provide the target application, biomolecule, detection instrument and required quantity when requesting customization.

Quality Control and Documents

Available product documents may include:

• Certificate of Analysis

• Technical Data Sheet

• Safety Data Sheet

• Particle size report

• Particle size distribution data

• Fluorescence spectrum

• Carboxyl content data

• Dispersion stability data

• Batch inspection report

Why Choose SHBC?

Shanghai SanYu Biotechnology Co., Ltd. supplies fluorescent microspheres, colored latex particles, magnetic beads and other functional microsphere materials.

SHBC provides:

• Controlled microsphere manufacturing

• Stable particle size distribution

• Multiple fluorescence wavelengths

• Functional surface modification

• Batch-to-batch consistency

• Sample evaluation support

• Pilot and bulk production

• OEM and ODM services

Frequently Asked Questions

What is CFG300-10?

CFG300-10 is a 300 nm carboxyl-functionalized green fluorescent microsphere suspension with 1% solid content.

What are the excitation and emission wavelengths?

The excitation wavelengths are 470/488 nm, and the emission wavelength is 525 nm.

What is CFG300-10 mainly used for?

It is suitable for fluorescent lateral flow assays, fluorescence immunoassays, biosensors, antibody coupling and diagnostic reagent development.

Can antibodies be coupled to CFG300-10?

Yes. Antibodies and proteins containing primary amine groups can be covalently coupled to the carboxyl surface.

Which coupling chemistry can be used?

EDC or EDC/NHS chemistry can be used to activate the surface carboxyl groups.

Is CFG300-10 suitable for quantitative LFIA?

Yes. Particle loading, membrane type, conjugate release, running buffer and reader settings should be optimized.

Can CFG300-10 be excited by a 488nm laser?

Yes. Its specified excitation range includes 488 nm, with green emission near 525 nm.

How does 300nm compare with 200nm particles?

The 300 nm particles may provide more fluorescence and coupling area per particle. The 200 nm particles may provide faster membrane migration.

Can CFG300-10 be separated by centrifugation?

Yes. Centrifugation conditions should be optimized according to the buffer, particle concentration and required recovery.

Can CFG300-10 be customized?

Yes. Particle size, fluorescence wavelength, carboxyl density, solid content, dispersion medium and packaging can be customized.

Request a Sample or Quotation

Contact SHBC for CFG300-10 samples, technical specifications, coupling support and bulk pricing.

Please provide:

• Target application

• Target biomolecule

• Assay format

• Detection instrument

• Required quantity

• Preferred packaging

• Estimated annual demand

Product: 300nm Carboxyl Green-Fluo Microspheres
Catalog Number: CFG300-10
Particle Size: 300 nm
Solid Content: 1%
Excitation/Emission: 470/488–525 nm
Surface Group: Carboxyl, COOH
Brand: SHBC
Manufacturer: Shanghai SanYu Biotechnology Co., Ltd.