300nm Carboxyl Yellow-Fluo Microspheres 1% CFY300-10

300nm Carboxyl Yellow-Fluo Microspheres CFY300-10

SHBC CFY300-10 is a 300nm carboxyl functionalized yellow fluorescent microsphere designed for biological detection, biomolecule labeling and immunoassay development.

The microspheres provide stable yellow fluorescence with excitation wavelength at 465nm and emission wavelength at 510nm.

With reactive carboxyl groups (-COOH) on the particle surface, CFY300-10 enables covalent coupling with antibodies, proteins, peptides and other biomolecules through EDC/NHS chemistry.

CFY300-10 Product Specifications

Yellow Fluorescent Microsphere Optical Properties

CFY300-10 provides yellow fluorescence suitable for fluorescence-based detection platforms.

Optical characteristics:

• Excitation wavelength: 465nm

• Emission wavelength: 510nm

The defined fluorescence spectrum supports compatibility with fluorescence readers, imaging systems and analytical instruments.

Stable fluorescence intensity helps improve detection reliability and assay consistency.

Carboxyl Functionalized Surface Chemistry

CFY300-10 contains surface carboxyl groups that provide active sites for biomolecule immobilization.

The carboxyl groups can be activated using EDC/NHS coupling chemistry to form stable amide bonds with amino groups of proteins and antibodies.

Typical conjugation process:

Carboxyl Microsphere Activation → Biomolecule Coupling → Blocking → Fluorescent Conjugate Formation

Benefits include:

• Stable covalent attachment

• Reduced biomolecule leakage

• Improved assay reproducibility

• Flexible surface modification

Advantages of 300nm Yellow Fluorescent Beads

Controlled 300nm Particle Size

The 300nm particle size provides:

• Good dispersion stability

• High surface area

• Efficient biomolecule loading

• Suitable optical performance

• Reliable assay development

Functional Carboxyl Surface

The -COOH surface enables:

• Antibody immobilization

• Protein conjugation

• Peptide coupling

• Biomarker assay development

• Custom surface modification

Stable Yellow Fluorescence

Key fluorescence advantages:

• Bright yellow signal

• Stable emission wavelength

• Good batch consistency

• Compatibility with fluorescence detection systems

Applications in Immunoassay Development

CFY300-10 can be used as a fluorescent carrier for biological detection systems.

Potential applications include:

• Immunofluorescence assays

• Antibody labeling

• Antigen detection

• Biomarker analysis

• Diagnostic reagent development

• Research assay development

Carboxyl fluorescent microspheres are widely used as carriers for antibody and protein immobilization due to their convenient surface chemistry.

Antibody and Protein Conjugation with Carboxyl Microspheres

CFY300-10 supports covalent biomolecule coupling through surface carboxyl groups.

Compatible biomolecules include:

• Monoclonal antibodies

• Polyclonal antibodies

• Antigens

• Proteins

• Peptides

• Nucleic acids

Typical coupling workflow:

1. Microsphere washing

2. Carboxyl group activation using EDC/NHS

3. Addition of biomolecule

4. Covalent amide bond formation

5. Blocking remaining active sites

6. Performance evaluation

Fluorescent Microspheres for Lateral Flow and POCT

Yellow fluorescent microspheres can be evaluated as labeling materials for fluorescence-based lateral flow assays.

Potential advantages:

• Enhanced fluorescence detection

• Stable signal output

• Flexible antibody conjugation

• Suitable for rapid diagnostic development

Applications include:

• Infectious disease testing

• Clinical biomarkers

• Food safety detection

• Veterinary diagnostics

• Environmental monitoring

Fluorescent Beads for Flow Cytometry and Bioanalysis

CFY300-10 may be used as fluorescent particles for analytical applications.

Potential uses:

• Flow cytometry calibration

• Fluorescent bead standards

• Multiplex assay development

• Particle-based detection platforms

The controlled fluorescence wavelength allows integration with compatible optical detection systems.

Comparison of Carboxyl Fluorescent Microspheres

Storage and Handling Recommendations

For maintaining product performance:

• Avoid repeated freeze-thaw cycles

• Protect from strong light

• Mix gently before use

• Maintain uniform particle dispersion

• Store under recommended conditions

Proper storage helps maintain fluorescence stability and particle consistency.

Custom Fluorescent Microsphere Manufacturing

Shanghai SanYu Biotechnology Co., Ltd. provides customized fluorescent microsphere solutions.

Available options include:

• Different particle sizes

• Different fluorescence colors

• Different surface functional groups

• Customized solid content

• Bulk production supply

Available products include:

• Carboxyl fluorescent microspheres

• Amino fluorescent microspheres

• Magnetic fluorescent microspheres

• Standard fluorescent particles

Frequently Asked Questions

What are CFY300-10 microspheres?

CFY300-10 are 300nm carboxyl functionalized yellow fluorescent microspheres designed for biomolecule coupling and fluorescence detection applications.

What are the excitation and emission wavelengths?

The excitation wavelength is 465nm and emission wavelength is 510nm.

What functional group is available on the surface?

CFY300-10 contains carboxyl (-COOH) functional groups.

Can CFY300-10 be coupled with antibodies?

Yes. The carboxyl groups support antibody coupling through EDC/NHS activation chemistry.

What applications use yellow fluorescent microspheres?

They are commonly used in immunoassays, fluorescence labeling, lateral flow assays and bioanalysis.

What is the solid content?

The standard solid content is 1%.

Can SHBC provide customized fluorescent microspheres?

Yes. SHBC provides customized particle sizes, fluorescence colors, surface groups and bulk manufacturing services.

Request CFY300-10 Samples and Bulk Pricing

Contact Shanghai SanYu Biotechnology Co., Ltd. for samples, technical information and bulk quotations of 300nm Carboxyl Yellow-Fluo Microspheres CFY300-10.

CFY300-10 combines controlled particle size, stable yellow fluorescence and carboxyl surface chemistry for advanced biological detection and assay development.