Product Overview
SHBC Octyl TopHIC HP is a high-performance, high-resolution hydrophobic interaction chromatography (HIC) media designed for the separation and purification of proteins and other biomolecules.
The media is based on 6% cross-linked agarose with an average particle size of approximately 34 μm and uses an Octyl hydrophobic ligand.
Compared with the Fast Flow series, the smaller particle size provides higher chromatographic resolution, making Octyl TopHIC HP particularly suitable for intermediate and fine purification of biomolecules.
Key Features
34 μm average particle size
6% cross-linked agarose matrix
Octyl hydrophobic ligand
High-resolution HIC media
≈35 mg/mL dynamic binding capacity for BSA
Smaller particle size than FF series
Higher chromatographic resolution
Suitable for intermediate purification
Suitable for fine purification
Designed for protein and biomolecule separation
Technical Specifications
Parameter | Specification |
|---|
Product Name | Octyl TopHIC HP |
Brand | SHBC |
Product Type | High-Resolution Hydrophobic Interaction Chromatography Media |
Matrix | 6% Cross-Linked Agarose |
Ligand | Octyl |
Chromatography Mode | Hydrophobic Interaction Chromatography |
Average Particle Size | 34 μm |
Dynamic Binding Capacity | ≈35 mg/mL (BSA) |
Package | 25 mL / 100 mL / 300 mL / 1 L / 5 L / 10 L / 20 L |
SHBC Art.No. | Packaging Specification |
A3022 | 25 mL |
A3023 | 100 mL |
A3024 | 300 mL |
A3025 | 1 L |
A3026 | 5 L |
A3027 | 10 L |
A3028 | 20 L |
34 μm High-Resolution Agarose Matrix
Octyl TopHIC HP uses a 6% cross-linked agarose matrix with an average particle size of approximately 34 μm.
The smaller particle size provides higher chromatographic resolution compared with the FF fast-flow series.
This makes the HP series particularly suitable for:
Intermediate purification
Fine purification
Polishing processes
High-resolution protein separation
Separation of closely related biomolecules
Hydrophobic Interaction Chromatography
Hydrophobic interaction chromatography separates biomolecules according to differences in their surface hydrophobicity.
Under suitable high-salt conditions, hydrophobic regions of biomolecules interact with hydrophobic ligands immobilized on the chromatography matrix.
By changing the mobile-phase conditions, these interactions can be weakened, allowing biomolecules with different hydrophobic properties to be separated.
Octyl Hydrophobic Ligand
Octyl TopHIC HP uses an Octyl hydrophobic ligand.
The Octyl ligand provides hydrophobic interaction selectivity for proteins and other biomolecules, while the fine 34 μm agarose matrix provides enhanced chromatographic resolution.
This combination makes Octyl TopHIC HP useful when fine separation of biomolecules with different hydrophobic properties is required.
Dynamic Binding Capacity
The specified reference dynamic binding capacity of Octyl TopHIC HP is:
≈35 mg/mL (BSA)
Bovine Serum Albumin (BSA) is used as the reference protein for the binding-capacity specification of this product.
Actual binding performance can vary depending on the properties of the target biomolecule and chromatographic conditions.
High Resolution for Fine Purification
The High Performance series is designed to provide higher resolution than the FF series.
Its 34 μm average particle size provides enhanced separation efficiency, making Octyl TopHIC HP suitable when the purification process requires:
Applications
SHBC Octyl TopHIC HP can be used for:
Protein purification
Recombinant protein purification
Biomolecule separation
Hydrophobic interaction chromatography
Intermediate purification
Fine purification
Polishing chromatography
Separation based on hydrophobicity
Biopharmaceutical downstream processing
Chromatography method development
Product Advantages
Octyl TopHIC HP combines:
High chromatographic resolution
Fine 34 μm particle size
6% cross-linked agarose matrix
Octyl hydrophobic selectivity
≈35 mg/mL BSA binding capacity
Suitable for intermediate purification
Suitable for fine purification
Suitable for protein and biomolecule separation
Octyl TopHIC HP vs. Octyl TopHIC 4FF
Both products use Octyl hydrophobic ligands but are designed for different purification priorities.
Feature | Octyl TopHIC HP | Octyl TopHIC 4FF |
Series | High Performance | Fast Flow |
Ligand | Octyl | Octyl |
Matrix | 6% Cross-Linked Agarose | 4% Highly Crosslinked Agarose |
Average Particle Size | 34 μm | 90 μm |
Reference Binding Capacity | ≈35 mg/mL BSA | ≈7 mg/mL HSA |
Main Positioning | High Resolution | Fast Flow |
Typical Application | Fine / Intermediate Purification | Process / General Purification |
Choose Octyl TopHIC HP when higher chromatographic resolution and fine purification are the main priorities.
Choose Octyl TopHIC 4FF when faster processing and general process purification are more important.
Storage and Handling
Keep Octyl TopHIC HP in its original container and protect the chromatography media from contamination during storage and handling.
Before use, equilibrate the packed bed with an appropriate buffer according to the intended HIC purification process.
Salt concentration, salt type, pH, sample loading and elution conditions should be optimized according to the target biomolecule.
Frequently Asked Questions
What is Octyl TopHIC HP?
Octyl TopHIC HP is a high-resolution hydrophobic interaction chromatography media based on 6% cross-linked agarose.
What is the average particle size?
The average particle size is approximately 34 μm.
What ligand does Octyl TopHIC HP use?
It uses an Octyl hydrophobic ligand.
What is the dynamic binding capacity?
The specified dynamic binding capacity is approximately 35 mg/mL for BSA.
What is the matrix?
The matrix is 6% cross-linked agarose.
What does HP mean?
HP represents the High Performance / high-resolution series, designed with smaller particles for improved chromatographic resolution.
Is Octyl TopHIC HP higher resolution than the FF series?
Yes. The HP series uses smaller particles and is designed to provide higher chromatographic resolution than the FF series.
What is Octyl TopHIC HP mainly used for?
It is mainly used for protein purification, intermediate purification, fine purification and hydrophobic interaction chromatography.
Is it suitable for fine purification?
Yes. The High Performance series is specifically positioned for intermediate or fine purification of biomolecules.
What package sizes are available?
SHBC offers 25 mL, 100 mL, 300 mL, 1 L, 5 L, 10 L and 20 L packages.
What are the SHBC Art.No. numbers?
The corresponding SHBC Art.No. are A3022, A3023, A3024, A3025, A3026, A3027 and A3028.
What brand is Octyl TopHIC HP?
Octyl TopHIC HP is supplied under the SHBC brand.
