7um Flow Red-Fluo Microspheres RCY7UM-630 0.5 %

7µm Flow Red-Fluo Microspheres for Flow Cytometry

Shanghai SanYu Biotechnology Co., Ltd. supplies RCY7UM-630 7µm Flow Red-Fluo Microspheres for red-laser flow cytometry research, fluorescence channel evaluation, instrument method development, bead-based assay research, and bulk reagent production.

RCY7UM-630 microspheres have a nominal particle diameter of 7µm and are designed for excitation at approximately 630nm. They provide a broad far-red fluorescence emission range of 665–720nm.

The product is supplied as a 0.5% solids suspension and can support laboratory evaluation, flow cytometry project development, pilot-scale testing, repeated production, and bulk purchasing.

Quick Product Answer

RCY7UM-630 is a 7µm far-red fluorescent microsphere suspension with 0.5% solids. It is designed for approximately 630nm excitation and 665–720nm emission and can be evaluated in red-laser flow cytometry research, fluorescence detection studies, bead-based assay development, instrument-setting research, and fluorescent microsphere reagent production.

Product Highlights

• Product name: 7µm Flow Red-Fluo Microspheres

• Catalog number: RCY7UM-630

• Manufacturer: Shanghai SanYu Biotechnology Co., Ltd.

• Nominal particle diameter: 7µm

• Excitation wavelength: 630nm

• Emission wavelength: 665–720nm

• Fluorescence region: Far red

• Solids content: 0.5%

• Recommended detection method: Flow cytometry

• Recommended laser region: Red laser

• Supply capability: Samples, pilot batches, and bulk production

• Intended use: Research use only

What Are RCY7UM-630 Flow Red-Fluo Microspheres?

RCY7UM-630 consists of 7µm fluorescent microspheres that produce a far-red fluorescence signal when excited by a compatible red laser.

The 7µm particle diameter is within the micron-size range commonly evaluated in conventional flow cytometry research. Compared with nanoparticles and submicron beads, 7µm microspheres may produce stronger forward-scatter and side-scatter signals, making the main particle population easier to locate and gate on many flow cytometers.

The combination of micron-scale particle size and far-red fluorescence makes RCY7UM-630 suitable for research involving:

• Red-laser fluorescence detection

• Far-red detector evaluation

• Flow cytometer setting optimization

• Fluorescence threshold development

• Scatter and fluorescence gating

• Particle dilution studies

• Event-rate optimization

• Flow cytometry method development

• Bead-based assay research

• Fluorescent particle tracking

• Instrument comparison

• Research reagent manufacturing

RCY7UM-630 can be evaluated by biotechnology companies, flow cytometry laboratories, universities, research institutes, instrument manufacturers, reagent developers, and organizations developing particle-based analytical methods.

The standard product is supplied for research use. It should not automatically be represented as a certified calibration material, absolute counting standard, MESF standard, or regulated diagnostic control unless the relevant qualification and documentation are specifically provided.

RCY7UM-630 Technical Specifications

Lot-specific parameters should be confirmed using the corresponding product specification or Certificate of Analysis.

Depending on the confirmed order specification, supporting information may include:

• Nominal particle diameter

• Particle-size distribution

• Solids content

• Suspension appearance

• Fluorescence excitation information

• Fluorescence emission information

• Lot number

• Production date

• Storage recommendations

• Handling instructions

• Certificate of Analysis

• Safety Data Sheet

Particle matrix, surface chemistry, preservative system, particle-size coefficient of variation, fluorescence intensity range, shelf life, packaging size, and storage buffer should be confirmed before publication when these values are available.

630nm Excitation and 665–720nm Far-Red Emission

RCY7UM-630 is designed for excitation near 630nm and produces fluorescence across the 665–720nm far-red region.

Potentially compatible red-laser wavelengths may include:

• 630nm

• 633nm

• 635nm

• 637nm

• 640nm

The product specification identifies 630nm as the excitation wavelength. Instruments using nearby red-laser wavelengths may also be suitable, but the final fluorescence response should be confirmed on the intended flow cytometer.

The 665–720nm emission range may overlap several far-red detector configurations.

Potential detector filter regions may include:

• Approximately 660–680nm

• Approximately 665–695nm

• Approximately 670–710nm

• Approximately 680–720nm

• Approximately 690–735nm

These ranges are general compatibility examples rather than guaranteed instrument settings.

The measured fluorescence signal can be affected by:

• Laser wavelength

• Laser power

• Optical alignment

• Detector sensitivity

• Detector voltage or gain

• Dichroic mirrors

• Bandpass filters

• Sample concentration

• Flow rate

• Trigger threshold

• Suspension buffer

• Instrument background

• Particle aggregation

Users should review the actual laser and filter specifications of the flow cytometer rather than relying only on channel names such as APC, FL4, far red, red fluorescence, or red-laser channel.

Why Choose 7µm Fluorescent Microspheres?

Clearly Detectable Micron-Size Particle Population

The nominal 7µm particle diameter can provide a distinct microsphere population on many conventional flow cytometers.

Compared with submicron particles, 7µm microspheres may be easier to separate from:

• Electronic noise

• Buffer particles

• Small debris

• Instrument background

• Low-scatter contaminants

Actual performance depends on the optical and fluidic configuration of the instrument.

Suitable for Conventional Flow Cytometers

Research involving nanoparticles or extracellular vesicles may require specialized small-particle detection settings.

By contrast, a 7µm fluorescent microsphere population can generally be evaluated using more conventional forward-scatter, side-scatter, and fluorescence plots.

This makes RCY7UM-630 suitable for initial flow cytometry method development and laboratory training projects.

Red-Laser Excitation

RCY7UM-630 provides a far-red fluorescence signal under approximately 630nm excitation.

It may be used when researchers need a fluorescent particle population that can be detected using a red laser and a compatible far-red detector.

Cell-Size-Range Research Model

The 7µm particle size falls within the broad size range of many biological cells and cell-like particles.

The microspheres may therefore be evaluated when researchers require a synthetic micron-sized population for instrument-setting or particle-handling studies.

RCY7UM-630 does not reproduce the deformability, refractive index, density, surface structure, fluorescence distribution, or biological behavior of a specific cell type. Suitability as a cell surrogate must be established for each application.

Suitable for Bead-Based Research

The micron-scale particle size may provide a practical platform for research involving bead-based detection, particle coding, biomolecule immobilization, and multiplex flow cytometry.

A suitable functional surface is required when antibodies, antigens, proteins, or nucleic acids need to be coupled to the particles.

Supports Research-to-Production Development

RCY7UM-630 can be evaluated in small quantities during early-stage research and subsequently transferred to pilot or bulk manufacturing after the required technical specification has been confirmed.

Key Features and Benefits

7µm Nominal Particle Diameter

The micron-scale particle size is suitable for flow cytometry detection, gating studies, particle tracking, and bead-based method development.

Far-Red Fluorescence

The product is designed for approximately 630nm excitation and 665–720nm emission.

Red-Laser Compatibility

RCY7UM-630 can be evaluated on flow cytometers equipped with compatible red-laser excitation.

Broad Emission Range

The broad far-red emission region provides flexibility when selecting compatible detector filters.

0.5% Solids Suspension

The supplied concentration allows users to prepare working dilutions according to the required event rate, acquisition time, and experimental design.

Visible Separation from Small Debris

The micron-sized population may be easier to distinguish from submicron background particles than smaller fluorescent microspheres.

Suitable for Multiple Research Workflows

RCY7UM-630 may be evaluated in flow cytometry, fluorescence microscopy, microfluidics, particle tracking, assay development, and instrument research.

Sample-to-Bulk Supply

Shanghai SanYu Biotechnology Co., Ltd. supports laboratory sample evaluation, pilot-scale development, repeated orders, and bulk manufacturing.

Custom Development Support

Alternative sizes, concentrations, fluorescence intensities, surface chemistries, packaging formats, and quality-control requirements may be discussed according to project feasibility.

Applications in Flow Cytometry Research

Red-Laser Fluorescence Detection

RCY7UM-630 may be used to evaluate red-laser excitation and far-red fluorescence detection.

Researchers can study:

• Particle fluorescence intensity

• Signal-to-background separation

• Detector sensitivity

• Detector voltage or gain

• Fluorescence histogram position

• Fluorescence distribution width

• Run-to-run stability

• Day-to-day instrument variation

Flow Cytometer Setup Research

The microspheres can support research involving consistent instrument setup.

Potential procedures include:

• Locating the microsphere population

• Adjusting forward-scatter settings

• Adjusting side-scatter settings

• Selecting the far-red detector

• Establishing a fluorescence threshold

• Setting an acquisition trigger

• Creating a particle gate

• Comparing different flow rates

• Evaluating event-rate stability

• Checking sample carryover

The standard product should not be described as a certified alignment or calibration bead unless the appropriate value assignment and qualification have been completed.

Scatter and Fluorescence Gating

RCY7UM-630 can be evaluated using several plot combinations:

• Forward scatter versus side scatter

• Forward scatter versus far-red fluorescence

• Side scatter versus far-red fluorescence

• Far-red fluorescence histogram

• Pulse area versus pulse height

• Pulse area versus pulse width

These plots may help distinguish:

• Single microspheres

• Possible aggregates

• Background events

• Coincident events

• Abnormally bright particles

• Low-fluorescence particles

Fluorescence Threshold Development

A fluorescence trigger may be useful when researchers want to acquire only events that produce a far-red fluorescence signal.

RCY7UM-630 may support studies involving:

• Fluorescence-triggered acquisition

• Trigger-threshold selection

• Background-event exclusion

• Scatter-trigger comparison

• Detection sensitivity evaluation

• Low-intensity event discrimination

Particle Dilution Studies

The 0.5% solids suspension should normally be diluted before flow cytometry testing.

A dilution series can help determine a working concentration that provides:

• Stable event rates

• Minimal coincidence

• Clear population separation

• Reproducible fluorescence

• Suitable acquisition time

• Low sample consumption

• Acceptable background

The appropriate dilution factor depends on the instrument, flow rate, sample volume, application, and desired number of recorded events.

Event-Rate and Coincidence Evaluation

When too many microspheres pass through the laser interrogation region at the same time, two or more particles may be recorded as one event.

Possible indications of coincidence include:

• Increased apparent fluorescence intensity

• Increased scatter intensity

• Broad particle distributions

• Unexpected doublet populations

• Concentration-dependent signal changes

• Unstable event rates

Testing multiple dilutions can help identify an appropriate working range.

Fluidic-System Research

Fluorescent microspheres can also support studies of sample movement through a flow cytometer or microfluidic system.

Potential observations include:

• Sample introduction

• Flow stability

• Event-rate consistency

• Particle recovery

• Sample carryover

• Tube-to-tube reproducibility

• Cleaning effectiveness

• Blockage or interruption

• Particle loss within the fluidic path

Fluorescence Microscopy

RCY7UM-630 may be evaluated using fluorescence microscopes equipped with a compatible red excitation source and far-red emission filter.

Possible microscopy applications include:

• Particle localization

• Particle distribution studies

• Microfluidic imaging

• Filtration studies

• Surface-interaction research

• Particle uptake experiments

• Fluorescence stability observations

Compatibility should be confirmed using the actual microscope filter set.

Compatible Red Lasers and Detection Channels

Recommended Excitation Region

A red laser close to 630nm is recommended.

RCY7UM-630 is not primarily designed for excitation with 405nm, 488nm, or 561nm lasers.

Signal under alternative excitation wavelengths should not be assumed unless supported by spectral testing.

Recommended Emission Detection

Select a detector filter that overlaps the 665–720nm emission range.

The filter should be evaluated according to both:

• Center wavelength

• Bandwidth

For example:

• A 670/30 filter collects approximately 655–685nm.

• A 680/30 filter collects approximately 665–695nm.

• A 695/40 filter collects approximately 675–715nm.

• A 710/50 filter collects approximately 685–735nm.

These examples describe wavelength coverage only. They do not guarantee equal fluorescence intensity on every instrument.

APC-Associated Channels

The emission range of RCY7UM-630 may overlap certain detector channels commonly used for APC-like fluorophores.

However, the microspheres should not be described as APC-labeled unless APC is actually used in the product.

Compatibility should be determined using the actual product excitation and emission spectrum.

Spectral Flow Cytometry

RCY7UM-630 may also be evaluated on compatible spectral flow cytometers.

For spectral workflows, users should collect the complete fluorescence signature under the intended instrument settings and build an appropriate reference spectrum.

The product should be tested independently before being used for spectral unmixing controls.

Flow Cytometry Instrument Setup and Method Development

RCY7UM-630 may support the establishment of reproducible flow cytometry procedures.

Initial Instrument Configuration

Confirm that the flow cytometer includes:

• A compatible red laser

• A detector overlapping 665–720nm

• Adjustable fluorescence gain or voltage

• Adjustable scatter settings

• Suitable acquisition software

• Clean and stable fluidics

Establishing a Particle Gate

A preliminary gate may be created using forward scatter and side scatter.

The far-red fluorescence signal can then be used to confirm the identity of the microsphere population.

The gate should exclude:

• Buffer background

• Electronic noise

• Debris

• Possible aggregates

• Coincident events

• Saturated events

Optimizing Detector Gain

Begin with a relatively low detector setting and increase the gain or voltage gradually.

The main population should be positioned within the measurable range without reaching detector saturation.

Record the final settings for future testing.

Monitoring Distribution Width

A narrow fluorescence distribution may indicate a relatively uniform measured population, while a broad distribution can result from:

• Particle aggregation

• Coincident events

• Instrument instability

• Incomplete mixing

• Excessively high sample concentration

• Detector saturation

• Variation within the microsphere population

Further investigation is required before attributing distribution width to a single cause.

Comparing Instruments

The same microsphere lot may be evaluated on different flow cytometers.

For meaningful comparison, record:

• Instrument model

• Laser wavelength

• Laser power

• Detector filter

• Detector setting

• Trigger channel

• Threshold

• Sample flow rate

• Dilution

• Buffer

• Acquisition time

• Number of collected events

Results from different instruments may not be directly comparable without an appropriate standardization method.

Bead-Based Flow Cytometry Assay Development

RCY7UM-630 may be considered as a fluorescent particle platform for bead-based flow cytometry research.

Before developing a coupling procedure, confirm the actual particle surface chemistry.

Possible surface options for customized projects may include:

• Plain surface

• Carboxyl-functionalized surface

• Amino-functionalized surface

• Streptavidin-coated surface

• Protein-coated surface

• Other project-specific functional groups

Availability depends on technical feasibility.

Potential Biomolecules

With an appropriate functional surface, fluorescent microspheres may be evaluated for immobilizing:

• Antibodies

• Antigens

• Proteins

• Peptides

• Enzymes

• Oligonucleotides

• DNA probes

• RNA probes

• Receptors

• Ligands

Coupling Development Parameters

Important factors may include:

• Surface functional group

• Activation chemistry

• Coupling-buffer composition

• Buffer pH

• Biomolecule concentration

• Microsphere concentration

• Activation time

• Coupling time

• Mixing method

• Blocking reagent

• Washing conditions

• Storage buffer

• Preservative

• Target sample matrix

• Non-specific adsorption

• Fluorescence retention

The coupling process should be optimized using the actual biomolecule and application.

Multiplex Assay Development

Several bead populations may potentially be distinguished using:

• Particle size

• Fluorescence color

• Fluorescence intensity

• Internal fluorescence code

• Surface chemistry

• Reporter fluorescence

For multiplex assay research, each bead population should remain distinguishable after:

• Biomolecule coupling

• Blocking

• Washing

• Storage

• Sample incubation

• Reporter labeling

• Flow cytometry acquisition

The reporter channel should also be sufficiently separated from the RCY7UM-630 far-red fluorescence signal.

Immunoassay Research

Functionalized 7µm microspheres may potentially be evaluated in bead-based immunoassay development.

A typical research workflow may include:

1. Microsphere activation or coating.

2. Capture biomolecule immobilization.

3. Blocking of unreacted surfaces.

4. Incubation with the target sample.

5. Addition of a detection reagent.

6. Washing.

7. Flow cytometry acquisition.

8. Data analysis.

The standard RCY7UM-630 product should not be assumed to contain a coupling-ready surface unless the functional group is specifically confirmed.

Comparison with 3µm and 500nm Fluorescent Microspheres

RCY7UM-630, RCY3UM-630, and 500nm Flow Red-Fluo Microspheres can serve different flow cytometry research priorities.

Choose 7µm RCY7UM-630 When:

• A larger micron-sized fluorescent population is preferred.

• Strong scatter visibility is important.

• A cell-size-range synthetic particle is required.

• The project involves red-laser detector setup.

• A bead-based assay platform is being developed.

• Easier separation from small particulate background is desired.

• A larger particle surface is preferred for a customized coupling project.

Choose 3µm RCY3UM-630 When:

• A smaller micron-sized bead is preferred.

• The project requires a balance between scatter detection and smaller particle size.

• A compact bead-based flow cytometry platform is being considered.

• Lower settling behavior than larger beads is preferred.

Choose 500nm Microspheres When:

• The project focuses on submicron particle detection.

• Small-particle flow cytometry is the primary objective.

• Fluorescence-triggered acquisition is being developed.

• The instrument has suitable submicron detection capability.

• Extracellular-vesicle-related method research is being performed.

The final particle size should be selected according to instrument capability, assay design, desired surface area, suspension behavior, and target application.

Recommended Preparation and Testing Workflow

The following workflow is a general starting point and should be optimized for the intended flow cytometer and research application.

1. Prepare the Instrument

Clean and prime the flow cytometer according to the manufacturer’s instructions.

Confirm that:

• The fluidic system is stable.

• The red laser is operating.

• The far-red detector is available.

• The waste and sheath systems are ready.

• The sample line is free of carryover.

2. Prepare a Buffer Blank

Use the same buffer that will be used to dilute RCY7UM-630.

Run the buffer blank before the microspheres and record:

• Forward-scatter background

• Side-scatter background

• Far-red fluorescence background

• Total event rate

• Any abnormal particulate population

3. Resuspend RCY7UM-630

Micron-sized microspheres may settle during storage.

Before sampling:

1. Allow the vial to reach the recommended handling temperature.

2. Gently invert the container several times.

3. Apply controlled vortexing when necessary.

4. Confirm that the suspension appears homogeneous.

5. Avoid producing excessive foam.

Brief mild bath sonication may be evaluated if the product documentation permits it and ordinary mixing does not provide sufficient redispersion.

4. Prepare Working Dilutions

Do not begin with the undiluted 0.5% suspension.

Prepare several working dilutions using a clean and compatible buffer.

A series of stepwise or tenfold dilutions can help determine the suitable event-rate range.

5. Start with a Low Flow Rate

Use a low sample flow rate during the initial test.

A lower flow rate may improve population resolution and reduce coincidence.

Higher flow rates can be evaluated after suitable settings have been established.

6. Locate the Microsphere Population

Use forward scatter and side scatter to locate the micron-sized particle population.

Confirm particle identity using the far-red fluorescence channel.

7. Optimize the Far-Red Detector

Select a detector that overlaps 665–720nm.

Adjust the detector gain or voltage so that the population:

• Is clearly separated from the blank

• Remains within the measurable range

• Does not reach saturation

• Can be consistently gated

8. Exclude Aggregates and Doublets

Evaluate pulse geometry using appropriate parameters such as:

• Area

• Height

• Width

Possible aggregates may show increased scatter, fluorescence, or pulse width.

9. Evaluate Multiple Dilutions

Compare the fluorescence and scatter distributions of several dilutions.

A suitable working dilution should produce stable measurements without excessive coincidence.

10. Record the Complete Method

For reproducibility, record:

• Product catalog number

• Product lot number

• Flow cytometer model

• Laser wavelength

• Detector filter

• Detector voltage or gain

• Scatter settings

• Trigger channel

• Threshold

• Sample flow rate

• Dilution factor

• Buffer composition

• Mixing procedure

• Acquisition time

• Number of collected events

• Analysis gate

Quality Control and Batch-to-Batch Consistency

Research organizations purchasing fluorescent microspheres in bulk usually require defined and repeatable product specifications.

Potential quality-control parameters may include:

• Nominal particle diameter

• Particle-size distribution

• Particle-size coefficient of variation

• Suspension appearance

• Solids content

• Dispersion performance

• Aggregate level

• Excitation characteristics

• Emission characteristics

• Relative fluorescence intensity

• Fluorescence distribution

• Flow cytometry histogram position

• Lot-to-lot comparison

• Storage stability

• Packaging integrity

• Microbial control

Not every parameter is necessarily included in a standard product specification.

Required release tests and acceptance limits should be agreed upon before pilot or bulk production.

Flow Cytometry Performance Evaluation

A flow cytometry QC method may evaluate:

• Position of the main microsphere population

• Median or mean fluorescence intensity

• Fluorescence distribution width

• Percentage of events within the main gate

• Scatter distribution

• Aggregate population

• Blank separation

• Repeatability between runs

The same instrument and acquisition settings should be used when comparing production lots.

Batch-to-Batch Consistency

For repeated production, customers may request control of:

• Particle diameter

• Particle-size distribution

• Fluorescence intensity

• Fluorescence distribution

• Solids content

• Buffer composition

• Suspension stability

• Packaging configuration

Shanghai SanYu Biotechnology Co., Ltd. can discuss project-specific quality requirements before manufacturing.

Calibration and Certification Claims

Standard RCY7UM-630 should not automatically be represented as:

• A NIST-traceable size standard

• A certified fluorescence standard

• A MESF-assigned bead

• An ERF-assigned reference material

• An absolute counting bead

• A certified flow cytometer calibration bead

• A regulated diagnostic control

These applications require specific value assignment, testing, uncertainty analysis, traceability, and supporting documentation.

Bulk Manufacturing and Customization

Shanghai SanYu Biotechnology Co., Ltd. supports fluorescent microsphere projects from laboratory evaluation to bulk production.

Research Sample Evaluation

Small quantities may be evaluated for:

• Red-laser compatibility

• Far-red detector compatibility

• Particle dispersion

• Scatter detection

• Fluorescence intensity

• Event-rate optimization

• Assay feasibility

• Instrument compatibility

Pilot-Scale Production

Pilot batches can support:

• Method optimization

• Initial stability studies

• Packaging evaluation

• Quality-control development

• Coupling feasibility

• Customer verification

• Process transfer

Bulk Production

After the technical specification is confirmed, bulk production can be arranged according to the customer’s purchasing plan and annual forecast.

Project information should include:

• Required quantity

• Expected annual demand

• Preferred package size

• Intended application

• Flow cytometer configuration

• Required fluorescence level

• Surface chemistry

• Buffer requirements

• Quality specifications

• Documentation requirements

• Delivery schedule

• Delivery destination

Custom Particle Size

Alternative fluorescent microsphere diameters may be discussed for qualified projects.

Possible development directions include:

• Nanometer-scale particles

• Submicron particles

• Small micron-sized beads

• Cell-size-range microspheres

• Larger fluorescent microspheres

Custom Fluorescence

Depending on feasibility, customization may include:

• Alternative excitation wavelengths

• Alternative emission wavelengths

• Different fluorescence colors

• Adjusted fluorescence intensity

• Multiple fluorescence-intensity levels

• Multiple fluorescent codes

Custom Surface Chemistry

Possible project-specific surface options may include:

• Plain microspheres

• Carboxyl microspheres

• Amino microspheres

• Streptavidin-coated microspheres

• Protein-coated microspheres

• Other functional surfaces

Custom Concentration and Packaging

Alternative solids concentrations and packaging formats may be discussed according to order quantity and technical feasibility.

Available options may include:

• Laboratory sample packaging

• Pilot-scale packaging

• Bulk containers

• Customer-specific fill volumes

• OEM labeling

• Customer-specific product codes

Handling and Storage Recommendations

Follow the product label, technical data sheet, and Certificate of Analysis supplied with the order.

General handling recommendations include:

• Store protected from light.

• Refrigerated storage at 2–8°C is generally recommended unless otherwise specified.

• Do not freeze unless freeze-thaw stability has been confirmed.

• Keep the container tightly closed.

• Store the vial upright.

• Mix thoroughly before sampling.

• Use clean pipette tips and sample tubes.

• Avoid contaminating the original suspension.

• Minimize prolonged exposure to strong light.

• Avoid repeated unnecessary temperature changes.

• Do not allow the microspheres to dry.

• Do not return diluted material to the original bottle.

Settling and Resuspension

Because RCY7UM-630 contains 7µm particles, settling may occur during storage.

Settling alone does not necessarily indicate product failure.

Before use:

1. Gently invert the container.

2. Apply controlled vortexing when necessary.

3. Confirm that the suspension is homogeneous.

4. Inspect for irreversible aggregates.

5. Test a small working dilution before the full experiment.

Avoiding Aggregation

Aggregation may be affected by:

• Incompatible buffers

• High salt concentrations

• Extreme pH

• Repeated freezing and thawing

• Drying

• Microbial contamination

• Prolonged storage after dilution

• Incompatible proteins or additives

Evaluate buffer compatibility when developing a new application.

Light Protection

Keep the original product and working dilutions protected from unnecessary light exposure.

Prepare working dilutions shortly before use when possible.

Frequently Asked Questions

What is RCY7UM-630?

RCY7UM-630 is a 7µm far-red fluorescent microsphere suspension developed for flow cytometry research. It has approximately 630nm excitation, 665–720nm emission, and 0.5% solids.

Who manufactures RCY7UM-630?

RCY7UM-630 is supplied by Shanghai SanYu Biotechnology Co., Ltd.

What is the nominal particle diameter?

The nominal particle diameter is 7µm.

Lot-specific size information should be confirmed using the relevant product specification or Certificate of Analysis.

What is the excitation wavelength?

The specified excitation wavelength is 630nm.

What is the emission wavelength?

The specified fluorescence emission range is 665–720nm.

Which flow cytometry laser should be used?

A compatible red laser near 630nm is recommended.

Red lasers at 633, 635, 637, or 640nm may also be evaluated, but the fluorescence response should be confirmed experimentally.

Which detector should be selected?

Select a far-red detector whose optical filter overlaps the 665–720nm emission range.

Review the actual bandpass filter rather than relying only on the detector channel name.

Can it be detected using an APC channel?

The emission range may overlap certain APC-associated detector configurations.

However, RCY7UM-630 should not be described as APC-labeled unless APC is actually used in the product.

Can it be excited by a 488nm laser?

RCY7UM-630 is specified for approximately 630nm excitation.

Adequate excitation at 488nm should not be assumed without supporting spectral data.

Is the product suitable for conventional flow cytometers?

The 7µm particle diameter can be evaluated on many conventional flow cytometers with compatible red-laser excitation and far-red detection.

Actual performance depends on the instrument configuration.

Why use 7µm microspheres?

The larger micron-sized particles may provide stronger scatter signals and easier separation from small background particles than submicron microspheres.

They may also be suitable for cell-size-range particle studies and bead-based assay development.

Are the microspheres equivalent to biological cells?

No.

Although the particle diameter may fall within the broad size range of some cells, synthetic microspheres do not reproduce all physical and biological properties of cells.

Can RCY7UM-630 be used for flow cytometer alignment?

It may be evaluated in instrument-setting and comparative alignment research.

It should not be represented as a certified alignment standard without the required qualification and documentation.

Is RCY7UM-630 a calibration bead?

The standard product is a research fluorescent microsphere.

Formal calibration applications require appropriate value assignment, traceability, acceptance limits, and supporting documentation.

Can it be used for absolute counting?

The 0.5% solids value does not establish a verified number of particles per milliliter.

Do not use RCY7UM-630 as an absolute counting standard unless a validated particle-number concentration has been supplied.

Can the microspheres be used for compensation?

The product may be evaluated during fluorescence-channel research.

Formal compensation controls should match the assay fluorophore and staining characteristics as closely as possible. Suitability must be validated experimentally.

Can antibodies be coupled to the particles?

Antibody coupling requires an appropriate functional surface.

Confirm whether a carboxyl, amino, streptavidin, or other coupling surface is required.

Can RCY7UM-630 be used for bead-based immunoassays?

A suitably functionalized version may be evaluated for bead-based immunoassay research.

The surface chemistry, coupling method, blocking conditions, reporter channel, and assay matrix must be optimized.

Can it be used in multiplex flow cytometry?

Yes, RCY7UM-630 may be evaluated as one particle population in a multiplex research system.

The far-red identification signal must remain distinguishable from the other microsphere populations and reporter fluorescence.

What is the supplied solids content?

RCY7UM-630 is supplied at 0.5% solids.

Should the product be diluted before flow cytometry?

Yes. A working dilution should normally be prepared before acquisition.

Testing several dilutions is recommended to determine a suitable event rate and minimize coincidence.

Why do the particles settle?

Micron-sized particles may settle during storage because of their size and density.

Mix the suspension thoroughly before sampling.

How should the product be mixed?

Gentle inversion followed by controlled vortexing can be used to redisperse the microspheres.

Avoid excessive foaming and prolonged harsh treatment.

Can the product be frozen?

Freezing is generally not recommended unless the supplied documentation specifically confirms freeze-thaw stability.

How should RCY7UM-630 be stored?

Store protected from light and follow the product-specific storage instructions.

Refrigerated storage is generally recommended unless otherwise specified.

Is bulk production available?

Yes. Shanghai SanYu Biotechnology Co., Ltd. supports sample evaluation, pilot production, repeat orders, and bulk manufacturing.

Can the concentration be customized?

Alternative concentrations may be discussed according to project requirements, technical feasibility, and order quantity.

Can the fluorescence intensity be customized?

Different fluorescence-intensity levels may be discussed for qualified development projects.

Can the particle surface be customized?

Plain, carboxyl, amino, streptavidin-coated, or other functional surfaces may be discussed according to application and technical feasibility.

What information is required for a quotation?

Please provide:

• Product catalog number

• Required quantity

• Expected annual demand

• Preferred package size

• Flow cytometer model

• Red-laser wavelength

• Far-red detector filter

• Intended application

• Required surface chemistry

• Required fluorescence intensity

• Quality-control requirements

• Documentation requirements

• Delivery destination

Request a Sample or Bulk Quotation

RCY7UM-630 7µm Flow Red-Fluo Microspheres provide a micron-sized far-red fluorescent particle solution for red-laser flow cytometry research, instrument-setting studies, fluorescence channel evaluation, bead-based assay development, particle tracking, and research reagent manufacturing.

The product provides:

• 7µm nominal particle diameter

• Approximately 630nm excitation

• 665–720nm far-red emission

• 0.5% solids

• Compatibility evaluation with red-laser flow cytometers

• Sample and pilot supply

• Bulk production capability

• Custom development support

For sample evaluation or bulk purchasing, provide the intended application, flow cytometer configuration, required quantity, surface chemistry, packaging format, and quality-control requirements.

Product Name: 7µm Flow Red-Fluo Microspheres
Catalog Number: RCY7UM-630
Manufacturer: Shanghai SanYu Biotechnology Co., Ltd.
Nominal Particle Diameter: 7µm
Excitation Wavelength: 630nm
Emission Wavelength: 665–720nm
Solids Content: 0.5%
Primary Application: Flow Cytometry Research
Supply Capability: Samples, Pilot Batches, and Bulk Production
Intended Use: Research Use Only. Not for diagnostic or therapeutic use.