Fmoc-Asn(Trt)-Wang Resin CAS 132388-59-1 0.3~0.8 Mmol/g, 100~200 Mesh, 1% DVB

Fmoc-Asn(Trt)-Wang Resin for C-Terminal Asparagine Peptides

SHBC Fmoc-Asn(Trt)-Wang Resin is a preloaded solid support for Fmoc solid-phase peptide synthesis of peptides containing C-terminal L-asparagine.

Fmoc-L-Asn(Trt)-OH is attached to an acid-labile Wang linker on a 1% DVB-crosslinked polystyrene matrix. The Trt group protects the asparagine side-chain amide during peptide assembly.

After acidic cleavage and deprotection, the resin produces a peptide ending in L-Asn-COOH.

Product Specifications

CAS 132388-59-1 identifies free Fmoc-Asn(Trt)-OH. Product-specific identification of the polymer-bound resin should follow the SHBC COA and SDS.

Key Advantages

Ready-to-Use Preloaded Support

Fmoc-L-Asn(Trt)-OH is already attached to the Wang linker, eliminating the initial asparagine loading step.

Defined C-Terminal Asparagine

The resin is designed for peptides that require L-asparagine as the C-terminal residue and a free carboxylic acid after cleavage.

Trt-Protected Side-Chain Amide

The trityl group protects the asparagine side-chain amide during repeated Fmoc deprotection and amino acid coupling cycles.

Compatible with Fmoc SPPS

The Wang linkage and Trt protection are compatible with standard base-mediated Fmoc peptide-chain assembly.

Acidic Cleavage and Deprotection

A validated TFA-based cleavage system can release the peptide, remove Trt and generate the final C-terminal Asn-COOH functionality.

Controlled Loading Range

The 0.3–0.8 mmol/g loading range supports selection according to peptide length, aggregation tendency and required productivity.

Practical Resin Matrix

The 100–200 mesh, 1% DVB-crosslinked polystyrene provides useful swelling, reagent diffusion, filtration and mechanical handling.

How Fmoc-Asn(Trt)-Wang Resin Works

The protected C-terminal asparagine is supplied pre-attached to the Wang linker through an acid-labile ester bond.

A typical workflow includes:

1. Pre-swell the resin in a compatible SPPS solvent.

2. Remove the Fmoc group from resin-bound Asn(Trt).

3. Couple the next protected amino acid.

4. Continue repeated Fmoc deprotection and coupling cycles.

5. Wash the resin between reaction steps.

6. Cleave and deprotect the completed peptide using a validated acidic system.

7. Purify and characterize the resulting C-terminal asparagine peptide acid.

The Trt group remains in place during Fmoc synthesis and is normally removed during final acidic cleavage.

Applications

SHBC Fmoc-Asn(Trt)-Wang Resin can be used for:

• C-terminal asparagine peptide synthesis

• Fmoc solid-phase peptide synthesis

• Peptide acids

• Asparagine-containing peptide fragments

• Therapeutic peptide research

• Antigenic and epitope peptides

• Enzyme substrate peptides

• Peptide reference standards

• Peptide library synthesis

• Modified and labeled peptides

• Peptidomimetic development

• Bioconjugation intermediates

• Research-scale and batch peptide production

The resin is particularly useful when the target sequence is predetermined to end in L-Asn-COOH.

Selecting the Right Resin Loading

Loading affects synthesis capacity, reagent accessibility and peptide aggregation.

Lower loading may be preferred for:

• Long peptide sequences

• Hydrophobic peptides

• Branched peptides

• Aggregation-prone sequences

• Sterically demanding coupling reactions

• Difficult peptide acids

Higher loading may improve productivity for shorter and less demanding sequences.

Always use the lot-specific loading value stated in the COA for resin weighing and reagent calculations.

Comparison with Other SPPS Supports

Fmoc-Asn(Trt)-Wang Resin and free Fmoc-Asn(Trt)-OH are different products. One is a polymer-bound support; the other is a soluble amino acid derivative.

Quality Control and Storage

Recommended qualification parameters include:

• Asparagine loading

• Fmoc content

• Trt protection integrity

• L-Asparagine stereochemical purity

• D-Isomer content

• Dipeptide content

• Particle-size distribution

• DVB crosslinking percentage

• Moisture content

• Residual free Wang linker sites

• Swelling performance

• Fmoc deprotection response

• Test-coupling efficiency

• Cleavage and Trt-removal performance

• Lot-to-lot consistency

Keep the resin tightly sealed in a cool, dry environment according to the product label and SDS. Protect it from moisture and contamination.

Frequently Asked Questions

What is Fmoc-Asn(Trt)-Wang Resin?

It is a Wang-type polystyrene resin preloaded with Fmoc- and Trt-protected L-asparagine for Fmoc solid-phase peptide synthesis.

What C-terminus is produced after cleavage?

The final peptide contains L-asparagine with a free C-terminal carboxylic acid.

What is the purpose of the Trt group?

Trt protects the asparagine side-chain amide during peptide-chain assembly and is normally removed during final acidic cleavage.

Does asparagine need to be loaded before synthesis?

No. Fmoc-L-Asn(Trt)-OH is already attached to the Wang linker.

Is this resin the same as Fmoc-Asn(Trt)-OH?

No. The resin is a polymer-bound synthesis support. Fmoc-Asn(Trt)-OH is a soluble amino acid building block.

Is it compatible with automated peptide synthesis?

Yes. It can be used with compatible automated Fmoc-SPPS workflows after method optimization.

How is the peptide cleaved?

A validated TFA-based cleavage system is commonly used. Acid concentration, scavengers and reaction time depend on the peptide sequence and protecting groups.

Which loading should I select?

Lower loading is often considered for long, hydrophobic or aggregation-prone peptides. Higher loading may improve productivity for shorter sequences.

What information is required for a quotation?

Please provide the required quantity, target loading, peptide application, packaging requirement and required quality documents.

Request a Sample or Bulk Quote

SHBC supplies Fmoc-Asn(Trt)-Wang Resin for Fmoc-SPPS, peptide process development and batch production.

Contact Shanghai SanYu Biotechnology Co., Ltd. to request evaluation quantities, bulk packaging, lot-specific loading data, COA, SDS and quotation details.

For research and process-development use unless otherwise specified.