Product Overview
SHBC GST TopAC 4FF is an affinity chromatography resin specifically designed for purification of glutathione S-transferase (GST) and GST fusion proteins.
The chromatography medium is prepared by coupling glutathione to highly cross-linked agarose gel, providing selective affinity for GST-tagged proteins.
GST TopAC 4FF has a specified ligand density of 120–320 μmol GST/mL and features high pressure resistance, fast flow and mild operating conditions that are conducive to maintaining protein activity.
Key Features
Designed for GST-tagged protein purification
Suitable for GST fusion protein purification
Glutathione affinity ligand
Highly cross-linked agarose gel matrix
Ligand density: 120–320 μmol GST/mL
High pressure resistance
Fast flow
Mild operating conditions
Helps maintain protein activity
Suitable for one-step affinity purification
Packages from 25 mL to 20 L
Technical Specifications
Parameter | Specification |
|---|
Product Name | GST TopAC 4FF |
Brand | SHBC |
English Name | GST-tag Affinity Resin 4FF |
Series | 4FF |
Matrix | Highly Cross-linked Agarose Gel |
Ligand | Glutathione |
Ligand Density | 120–320 μmol GST/mL |
Chromatography Mode | Affinity Chromatography |
Target | GST / GST Fusion Proteins |
Main Application | GST-tagged Protein Purification |
Characteristics | High Pressure Resistance / Fast Flow / Mild Conditions |
Package | 25 mL / 100 mL / 300 mL / 1 L / 5 L / 10 L / 20 L |
SHBC Art.No. | Packaging Specification |
|---|
A5050 | 25 mL |
A5051 | 100 mL |
A5052 | 300 mL |
A5053 | 1 L |
A5054 | 5 L |
A5055 | 10 L |
A5056 | 20 L |
Glutathione Affinity Ligand
GST TopAC 4FF is prepared by coupling glutathione to a highly cross-linked agarose gel matrix.
The glutathione ligand provides the affinity interaction required for selective purification of GST and GST fusion proteins.
This makes the resin particularly suitable for recombinant proteins expressed with a GST tag.
Ligand Density
The specified ligand density of GST TopAC 4FF is:
120–320 μmol GST/mL
This specification represents ligand density according to the supplied product table.
It should not be interpreted as the dynamic protein binding capacity of the resin.
GST-Tagged Protein Purification
GST is commonly used as a fusion tag in genetic engineering for the expression of recombinant fusion proteins.
GST TopAC 4FF is specifically designed to utilize the affinity interaction between GST and immobilized glutathione for purification of GST-tagged target proteins.
According to the supplied product information, proteins from different sources containing GST or GST fusion structures can be further purified using this affinity medium.
One-Step Affinity Purification
The supplied product description states that GST TopAC 4FF can be used to obtain high-purity target proteins through one-step purification.
The specific interaction between GST and glutathione enables selective capture of GST-tagged proteins from suitable samples.
High Pressure Resistance
GST TopAC 4FF is described as having high pressure resistance.
This characteristic comes together with fast-flow performance, supporting chromatography purification workflows where efficient processing is required.
A specific numerical pressure-resistance value is not provided in the supplied information.
The supplied product information identifies fast flow rate as one of the characteristics of the GST affinity chromatography medium.
This makes GST TopAC 4FF suitable for efficient affinity purification of GST-tagged and GST fusion proteins.
Mild Purification Conditions
GST TopAC 4FF is designed to operate under mild purification conditions.
According to the supplied information, these mild operating conditions are beneficial for maintaining protein activity during purification.
This is especially useful when purification performance and preservation of biological activity are both important.
Applications
SHBC GST TopAC 4FF can be used for:
GST-tagged protein purification
GST fusion protein purification
Recombinant protein purification
Affinity chromatography
One-step affinity purification
Protein separation and purification
Protein research
Downstream protein purification
Product Advantages
GST TopAC 4FF provides:
Glutathione affinity ligand
Highly cross-linked agarose gel
120–320 μmol GST/mL ligand density
Specific affinity for GST-tagged proteins
Suitable for GST fusion proteins
High pressure resistance
Fast flow
Mild operating conditions
Helps preserve protein activity
Suitable for one-step purification
Package sizes up to 20 L
GST Affinity Chromatography
GST affinity chromatography uses the specific interaction between GST and glutathione to isolate GST-tagged proteins.
In GST TopAC 4FF, glutathione is coupled to the highly cross-linked agarose gel matrix. GST-tagged target proteins can interact with the immobilized ligand, enabling selective affinity purification.
This makes the medium suitable for recombinant protein workflows using GST as an affinity tag.
Available Package Sizes
GST TopAC 4FF is available in:
25 mL / 100 mL / 300 mL / 1 L / 5 L / 10 L / 20 L
The broad package range supports laboratory protein purification as well as larger-scale purification requirements.
Frequently Asked Questions
What is GST TopAC 4FF?
GST TopAC 4FF is a glutathione affinity chromatography resin designed for purification of GST-tagged and GST fusion proteins.
What is the English product name?
The supplied English name is GST-tag Affinity Resin 4FF.
What ligand is used?
The resin uses glutathione as the affinity ligand.
What matrix is used?
According to the supplied information, glutathione is coupled to a highly cross-linked agarose gel.
What is the ligand density?
The specified ligand density is 120–320 μmol GST/mL.
Is 120–320 μmol GST/mL the protein binding capacity?
No. The supplier table specifically identifies this value as Ligand Density, not dynamic protein binding capacity.
What proteins can GST TopAC 4FF purify?
It is specifically designed for purification of GST-tagged proteins and GST fusion proteins.
Can it be used for recombinant proteins?
Yes. The supplied information specifically discusses GST as a tag used in genetic engineering for recombinant fusion protein expression.
Can GST TopAC 4FF be used for one-step purification?
Yes. According to the supplied information, the medium can be used for one-step purification to obtain high-purity target proteins from suitable samples.
Does it have high pressure resistance?
Yes. The supplied product information describes the chromatography medium as having high pressure resistance, although a specific pressure value is not provided.
Is GST TopAC 4FF a fast-flow resin?
The supplied information states that the chromatography medium has a fast flow rate.
Why are mild operating conditions important?
The supplied information states that the mild operating conditions are conducive to maintaining protein activity.
Is the particle size specified?
No. The supplied information does not provide a particle-size specification, so no particle size is added.
What package sizes are available?
Available sizes are 25 mL, 100 mL, 300 mL, 1 L, 5 L, 10 L and 20 L.
What are the SHBC Art.No. numbers?
The corresponding Art.No. are A5050–A5056.
What brand is GST TopAC 4FF?
GST TopAC 4FF is supplied under the SHBC brand.
