Product Overview
SHBC HardHIC Phenyl (HS) is a high-rigidity agarose-based hydrophobic interaction chromatography (HIC) resin designed for the separation and purification of proteins and other biomolecules.
The resin uses a high-rigidity agarose matrix combined with a Phenyl hydrophobic ligand. The rigid matrix provides low backpressure and high flow capability, making the media particularly suitable for large-scale biomolecule separation and purification.
HardHIC Phenyl (HS) provides a reference dynamic binding capacity of approximately 27 mg/mL for BSA.
Key Features
High-rigidity agarose matrix
Phenyl hydrophobic ligand
HS specification
≈27 mg/mL dynamic binding capacity for BSA
Low backpressure
High flow capability
Suitable for protein purification
Suitable for biomolecule separation
Designed for large-scale purification
Multiple package sizes from 25 mL to 20 L
Technical Specifications
Parameter | Specification |
|---|
Product Name | HardHIC Phenyl (HS) |
Brand | SHBC |
Product Type | High Rigidity Agarose HIC Resin |
Matrix | High Rigidity Agarose |
Ligand | Phenyl |
Specification | HS |
Chromatography Mode | Hydrophobic Interaction Chromatography |
Dynamic Binding Capacity | ≈27 mg/mL (BSA) |
Main Characteristics | Low Backpressure / High Flow |
Package | 25 mL / 100 mL / 300 mL / 1 L / 5 L / 10 L / 20 L |
SHBC Art.No. | Packaging Specification |
|---|
A3057 | 25 mL |
A3058 | 100 mL |
A3059 | 300 mL |
A3060 | 1 L |
A3061 | 5 L |
A3062 | 10 L |
A3063 | 20 L |
High Rigidity Agarose Matrix
HardHIC Phenyl (HS) uses high-rigidity agarose as the chromatography matrix.
According to the supplied product information, the high-rigidity structure provides two important process characteristics:
Low backpressure
High flow rates
These characteristics make the resin suitable for purification processes where both chromatographic performance and processing efficiency are important.
Phenyl Hydrophobic Ligand
HardHIC Phenyl (HS) uses a Phenyl hydrophobic ligand for hydrophobic interaction chromatography.
The Phenyl ligand provides hydrophobic interaction with proteins and other biomolecules, allowing separation according to differences in their hydrophobic properties.
This makes the resin suitable for protein purification and other biomolecule separation processes.
Dynamic Binding Capacity
The specified reference dynamic binding capacity is:
≈27 mg/mL (BSA)
Bovine Serum Albumin (BSA) is used as the reference protein for the dynamic binding capacity listed for HardHIC Phenyl (HS).
Actual binding performance may vary according to the target biomolecule and chromatographic conditions.
Low Backpressure and High Flow
One of the main characteristics of the HardHIC series is its high-rigidity agarose matrix.
The supplied product information specifically highlights:
These properties make HardHIC Phenyl (HS) suitable for chromatography processes requiring efficient sample processing at larger purification scales.
Hydrophobic Interaction Chromatography
Hydrophobic interaction chromatography (HIC) separates proteins and other biomolecules based on differences in their hydrophobic properties.
HardHIC Phenyl (HS) provides a Phenyl-functionalized hydrophobic stationary phase combined with a mechanically rigid agarose matrix.
The resin can therefore be incorporated into downstream purification processes requiring hydrophobic selectivity and scalable chromatographic operation.
Applications
SHBC HardHIC Phenyl (HS) can be used for:
Protein purification
Recombinant protein purification
Biomolecule separation
Hydrophobic interaction chromatography
Biopharmaceutical downstream processing
Large-scale biomolecule purification
Process chromatography
Separation based on hydrophobic properties
Chromatography process development
Scale-up purification
Product Advantages
HardHIC Phenyl (HS) combines:
High-rigidity agarose matrix
Phenyl hydrophobic selectivity
≈27 mg/mL BSA binding capacity
Low backpressure
High flow capability
Suitable for large-scale separation
Suitable for large-scale purification
Package sizes up to 20 L
Designed for Large-Scale Purification
The HardHIC series is specifically positioned for large-scale separation and purification of biomolecules.
The high-rigidity agarose matrix helps maintain efficient chromatographic operation while providing low backpressure and high flow capability.
Available volumes from 25 mL to 20 L also support progression from initial evaluation to larger purification processes.
Available Package Sizes
HardHIC Phenyl (HS) is available in:
25 mL
100 mL
300 mL
1 L
5 L
10 L
20 L
The corresponding SHBC Art.No. ranges from A3057 to A3063.
Frequently Asked Questions
What is HardHIC Phenyl (HS)?
HardHIC Phenyl (HS) is a high-rigidity agarose-based hydrophobic interaction chromatography resin using a Phenyl ligand.
What matrix does HardHIC Phenyl (HS) use?
It uses a high-rigidity agarose matrix.
What ligand is used?
The hydrophobic ligand is Phenyl.
What is the dynamic binding capacity?
The specified reference dynamic binding capacity is approximately 27 mg/mL for BSA.
What are the main advantages of the high-rigidity agarose matrix?
According to the supplied product information, the matrix provides low backpressure and high flow rates.
Is HardHIC Phenyl (HS) suitable for large-scale purification?
Yes. The HardHIC series is specifically described as suitable for large-scale separation and purification of biomolecules.
What is HardHIC Phenyl (HS) used for?
Typical applications include protein purification, biomolecule separation, hydrophobic interaction chromatography and large-scale downstream purification.
Is it suitable for protein purification?
Yes. The Phenyl hydrophobic ligand makes the resin suitable for hydrophobic interaction-based protein separation and purification.
What package sizes are available?
Available packages are 25 mL, 100 mL, 300 mL, 1 L, 5 L, 10 L and 20 L.
What are the SHBC Art.No. numbers?
The corresponding SHBC Art.No. are A3057, A3058, A3059, A3060, A3061, A3062 and A3063.
What is the English product name?
The supplied English name is High Rigidity Agarose Phenyl HIC Resin HS.
What brand is HardHIC Phenyl (HS)?
HardHIC Phenyl (HS) is supplied under the SHBC brand.
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