Sieber Resin CAS 915706-90-0 100-200mesh，1%DVB

Sieber Amide Resin for Protected Peptide Amide Synthesis

SHBC Sieber Amide Resin is a highly acid-labile solid support for Fmoc solid-phase peptide synthesis (SPPS).

Its xanthenyl-based linker allows peptide amides to be released under mild acidic conditions. This makes the resin especially suitable for preparing protected peptide amide fragments for further modification, fragment coupling or conjugation.

The 100–200 mesh, 1% DVB-crosslinked polystyrene matrix provides practical swelling, reagent accessibility, filtration and washing performance.

Product Specifications

Key Advantages of SHBC Sieber Amide Resin

Mild Release of Peptide Amides

Peptides can be cleaved using dilute TFA conditions, reducing exposure to the strong acidic cleavage normally required by standard Rink Amide resins.

Preparation of Protected Peptide Fragments

Mild cleavage can release the peptide while retaining selected side-chain protecting groups. This is useful for downstream coupling, cyclization, labeling and conjugation.

Produces C-Terminal Primary Amides

Cleavage from the linker produces peptides with a C-terminal –CONH₂ group without an additional amidation step.

Compatible with Fmoc SPPS

The resin supports standard Fmoc deprotection and amino acid coupling cycles used in manual and automated peptide synthesis.

Accessible Xanthenyl Linker

The linker structure may facilitate the attachment of bulky amino acids or other sterically demanding building blocks.

Practical Resin Matrix

The 1% DVB-crosslinked polystyrene and 100–200 mesh particle size provide a useful balance between swelling, reagent diffusion, filtration and mechanical handling.

How Sieber Amide Resin Works

Sieber Amide Resin contains an Fmoc-protected xanthenyl amine linker attached to a crosslinked polystyrene support.

A typical synthesis workflow includes:

1. Pre-swell the resin in a compatible SPPS solvent.

2. Remove the Fmoc group from the linker.

3. Couple the protected C-terminal amino acid.

4. Perform repeated Fmoc deprotection and coupling cycles.

5. Wash the resin between reaction steps.

6. Cleave the peptide using validated dilute-TFA conditions.

7. Purify and characterize the protected peptide amide.

A commonly reported cleavage condition is approximately 1% TFA in DCM. Actual conditions must be optimized according to the peptide sequence and protecting groups.

Applications

SHBC Sieber Amide Resin can be used for:

• Fmoc solid-phase peptide synthesis

• C-terminal peptide amide synthesis

• Protected peptide amide fragments

• Acid-sensitive peptide synthesis

• Convergent peptide synthesis

• Peptide fragment coupling

• Cyclic peptide development

• Modified and labeled peptides

• Bioconjugation-ready peptide fragments

• Peptide library synthesis

• Therapeutic peptide research

• Antigenic and epitope peptides

• Secondary carboxamide support development

• Research-scale and batch peptide production

The resin is particularly useful when the peptide must be removed from the solid support before complete side-chain deprotection.

Sieber Amide Resin vs Other SPPS Resins

Choose Sieber Amide Resin when a C-terminal peptide amide and mild release from the solid support are both required.

Selecting the Right Resin Loading

Resin loading influences synthesis capacity, reagent accessibility and aggregation risk.

Lower loading may be preferred for:

• Long peptide sequences

• Hydrophobic peptides

• Branched peptides

• Sterically demanding amino acids

• Aggregation-prone sequences

Higher loading may increase batch productivity for shorter and less demanding peptides.

Please specify the target loading range when requesting a quotation. Lot-specific substitution should be confirmed through the Certificate of Analysis.

Quality Control and Storage

Recommended quality-control parameters include:

• Linker loading or substitution

• Fmoc content

• Particle-size distribution

• DVB crosslinking percentage

• Moisture content

• Swelling performance

• Initial amino acid coupling efficiency

• Mild-cleavage performance

• Filtration behavior

• Lot-to-lot consistency

Keep the resin tightly sealed in a cool, dry environment according to the product label and SDS. Protect it from moisture, contamination and prolonged exposure to unsuitable conditions.

Frequently Asked Questions

What is Sieber Amide Resin used for?

It is used in Fmoc SPPS to prepare C-terminal peptide amides, especially protected peptide amide fragments.

Why is Sieber Resin described as highly acid-labile?

Its xanthenyl linker can be cleaved with dilute TFA, allowing peptide release under milder conditions than standard Rink Amide resins.

What C-terminus is produced?

Cleavage releases a peptide containing a C-terminal primary amide, –CONH₂.

Can side-chain protecting groups remain after cleavage?

Selected protecting groups may be retained under optimized mild cleavage conditions. Compatibility must be confirmed for the specific protecting-group strategy.

What is the difference between Sieber and Rink Amide Resin?

Both produce peptide amides. Sieber Resin is more acid-labile and is preferred for protected fragments, while Rink Amide Resin is commonly used when full acidic cleavage and deprotection are required.

Can Sieber Resin produce secondary carboxamides?

The linker can be modified through an appropriate reductive alkylation process to support the synthesis of selected secondary carboxamides.

Is it suitable for automated peptide synthesis?

Yes. It can be used with compatible automated Fmoc-SPPS workflows after method optimization.

What information is needed for a quotation?

Please provide the required quantity, target loading, intended peptide application, packaging requirement and required quality documents.

Request a Sample or Bulk Quote

SHBC supplies Sieber Amide Resin for protected peptide amide synthesis, Fmoc-SPPS development and batch production.

Contact Shanghai SanYu Biotechnology Co., Ltd. to request evaluation quantities, bulk packaging, lot-specific loading data, COA, SDS and quotation details.

For research and process-development use unless otherwise specified.