TopGFCL-4B Cross-Linked Gel Filtration Media 90um

TopGF CL-4B Cross-Linked Agarose Gel Filtration Media

SHBC TopGF CL-4B is a cross-linked agarose gel filtration medium developed for the separation, purification and analysis of biological macromolecules.

Manufactured by cross-linking the TopGF 4B matrix with a bifunctional reagent, TopGF CL-4B provides improved physical and chemical stability. It can withstand high-temperature sterilization at 121°C and is suitable for processing polysaccharides, nucleic acids, viruses and other large biomolecules.

Product Overview

TopGF CL-4B is a porous size exclusion chromatography medium. Molecules are separated according to differences in molecular size rather than specific binding interactions.

The cross-linked matrix provides improved structural stability, flow performance and resistance to demanding operating conditions, making it suitable for laboratory research, process development and industrial purification.

Key Features and Benefits

• Cross-linked agarose gel filtration matrix

• Improved physical and chemical stability

• Withstands sterilization at 121°C

• Suitable for large biological molecules

• Broad macromolecule separation applications

• Gentle, non-binding separation mechanism

• Good flow and column handling performance

• Suitable for repeated purification processes

• Laboratory and bulk packaging available

• Consistent batch manufacturing

Product Specifications

How TopGF CL-4B Works

TopGF CL-4B separates molecules according to their ability to enter the pores of the gel matrix.

Large molecules cannot fully enter the internal pores and therefore move through the column more quickly. Smaller molecules enter more pores and remain in the column for a longer time.

This size-based separation mechanism does not require a specific ligand and helps preserve the biological activity of sensitive samples.

Recommended Applications

Polysaccharide Separation and Purification

Suitable for fractionating and purifying high-molecular-weight polysaccharides used in pharmaceutical, food, biotechnology and natural product research.

Nucleic Acid Purification

Can be used for the separation, cleanup and analysis of DNA, RNA and other nucleic acid samples.

Virus Purification

Suitable for virus particle purification, virus-related research, vaccine process development and analytical sample preparation.

Biological Macromolecule Fractionation

Used for separating macromolecules with different hydrodynamic sizes in research and process development.

Biomolecule Detection

Can be used in analytical workflows requiring size-based fractionation before downstream detection or characterization.

Process Development

Suitable for laboratory method development, pilot-scale purification and production process evaluation.

Advantages of Cross-Linked CL-4B Media

Enhanced Matrix Stability

Bifunctional cross-linking improves the strength and stability of the agarose matrix compared with conventional non-cross-linked TopGF 4B media.

High-Temperature Sterilization

TopGF CL-4B can tolerate sterilization at 121°C, supporting processes with strict microbial control requirements.

Gentle Separation Conditions

Gel filtration separates molecules without requiring strong adsorption or harsh elution conditions, helping protect sensitive biomolecules.

Suitable for Large Molecules

The porous matrix is designed for the fractionation and purification of polysaccharides, nucleic acids, viruses and other biological macromolecules.

Reliable Column Performance

The cross-linked structure supports stable bed volume, consistent flow and reliable chromatographic operation.

TopGF CL-4B vs. TopGF 4B

TopGF CL-4B is recommended when higher matrix stability, sterilization resistance and repeated process operation are required.

Recommended Operating Procedure

1. Mix the gel medium gently before use.

2. Remove the storage solution and wash with purified water or equilibration buffer.

3. Prepare a uniform gel slurry.

4. Pack the chromatography column according to the required bed height.

5. Equilibrate the column with the selected running buffer.

6. Clarify or filter the sample before loading.

7. Apply the sample without disturbing the packed bed.

8. Elute using the same buffer and collect fractions.

9. Analyze the target fractions using a suitable detection method.

10. Clean, sanitize and store the medium according to the validated process.

Column dimensions, sample volume and flow rate should be optimized according to the target molecule and required resolution.

Why Choose SHBC TopGF CL-4B

Stable Cross-Linked Matrix

Controlled cross-linking technology improves the physical and chemical performance of the agarose gel.

Reliable High-Temperature Resistance

The medium supports 121°C sterilization for applications requiring strict microbial control.

Designed for Biological Macromolecules

TopGF CL-4B is suitable for processing polysaccharides, nucleic acids, viruses and other large biological molecules.

Consistent Batch Production

SHBC applies controlled manufacturing and quality inspection procedures to support reliable batch-to-batch performance.

Flexible Supply Options

Laboratory samples, pilot quantities, bulk packaging and customized supply are available.

Technical Support

SHBC provides support for media selection, column packing, process development and application evaluation.

Custom Packaging and Bulk Supply

Shanghai SanYu Biotechnology Co., Ltd. supplies TopGF CL-4B for universities, research institutes, biotechnology companies, pharmaceutical manufacturers and purification process developers.

Available services include:

• Laboratory sample supply

• Pilot-scale quantities

• Bulk production

• Custom packaging volume

• OEM and ODM services

• Batch quality documentation

• Column packing guidance

• Purification process support

Please provide the target biomolecule, sample volume, column size and estimated annual demand when requesting a quotation.

Frequently Asked Questions

What is TopGF CL-4B?

TopGF CL-4B is a cross-linked agarose gel filtration medium used for separating biological molecules according to molecular size.

What samples can be purified with TopGF CL-4B?

Typical samples include polysaccharides, DNA, RNA, viruses and other biological macromolecules.

Can TopGF CL-4B withstand autoclaving?

Yes. TopGF CL-4B can withstand high-temperature sterilization at 121°C under validated operating conditions.

How is TopGF CL-4B different from TopGF 4B?

TopGF CL-4B uses a bifunctional cross-linking process that improves matrix strength, chemical resistance and sterilization tolerance.

Does TopGF CL-4B bind the target molecule?

TopGF CL-4B mainly separates molecules according to size rather than specific chemical binding.

Is TopGF CL-4B suitable for virus purification?

Yes. It can be evaluated for virus particle separation, purification, detection and vaccine process development.

Can SHBC provide bulk quantities?

Yes. SHBC supports sample evaluation, pilot orders, custom packaging and bulk manufacturing.

Storage and Handling

Store TopGF CL-4B according to the conditions stated in the product technical documentation.

Do not allow the gel medium to dry. Avoid freezing and microbial contamination. Mix gently before use and validate cleaning, sterilization and storage procedures for the specific process.

Request a Sample or Quotation

Contact Shanghai SanYu Biotechnology Co., Ltd. for samples, technical documentation, customized packaging or bulk pricing.

Product: TopGF CL-4B
Brand: SHBC
Product Type: Cross-linked agarose gel filtration medium
Sterilization Resistance: Up to 121°C
Main Applications: Polysaccharide, nucleic acid, virus and biomacromolecule purification
Manufacturer: Shanghai SanYu Biotechnology Co., Ltd.

For research and industrial purification use.