1um Streptavidin Microspheres SAPS1UM-10 1%

1µm Streptavidin Microspheres for Flow Cytometry

Shanghai SanYu Biotechnology Co., Ltd. supplies SAPS1UM-10 1µm Streptavidin Microspheres for flow cytometry assay development, biotinylated biomolecule immobilization, particle-based immunoassays, affinity capture research, and bulk reagent manufacturing.

SAPS1UM-10 microspheres have a nominal particle diameter of 1µm and are supplied as a 1% solids suspension. The microsphere surface is coated with streptavidin, enabling the particles to capture biotinylated antibodies, antigens, proteins, peptides, oligonucleotides, DNA, RNA, and other biotin-containing molecules.

The product can support laboratory feasibility studies, bead-assay optimization, pilot-scale development, repeated production, and bulk purchasing by biotechnology companies and research organizations.

Quick Product Answer

SAPS1UM-10 is a 1µm streptavidin-coated microsphere suspension with 1% solids. It is designed to immobilize biotinylated biomolecules for flow cytometry bead assays, immunoassay research, nucleic acid capture, affinity binding studies, and particle-based analytical method development.

Product Highlights

• Product name: 1µm Streptavidin Microspheres

• Catalog number: SAPS1UM-10

• Manufacturer: Shanghai SanYu Biotechnology Co., Ltd.

• Nominal particle diameter: 1µm

• Surface coating: Streptavidin

• Solids content: 1%

• Physical form: Aqueous microsphere suspension

• Primary application: Flow cytometry research

• Binding principle: Streptavidin–biotin affinity

• Supply capability: Samples, pilot batches, and bulk production

• Customization: Available according to project feasibility

• Intended use: Research use only

What Are SAPS1UM-10 Streptavidin Microspheres?

SAPS1UM-10 consists of micron-sized polymer microspheres with streptavidin immobilized on the particle surface.

Streptavidin-coated microspheres provide a convenient solid support for attaching biotinylated biomolecules without requiring the end user to perform direct covalent coupling to the particle surface.

A biotinylated molecule can be incubated with the microspheres and captured through the high-affinity streptavidin–biotin interaction. The prepared microspheres can then be used in particle-based binding assays, flow cytometry analysis, target capture, biomolecule immobilization, and assay-development research.

Potential biotinylated capture molecules include:

• Antibodies

• Antigens

• Recombinant proteins

• Peptides

• Enzymes

• Lectins

• Receptors

• Ligands

• Oligonucleotides

• DNA probes

• RNA probes

• Aptamers

• Biotinylated small molecules

The 1µm particle size provides a relatively high particle number per unit mass while maintaining a physical surface suitable for biomolecule immobilization.

Because 1µm particles are near the lower detection range of some conventional flow cytometers, instrument compatibility and acquisition settings should be evaluated during method development.

SAPS1UM-10 Technical Specifications

The following parameters should be confirmed using the final technical specification or lot-specific Certificate of Analysis:

• Particle matrix

• Measured particle diameter

• Particle-size distribution

• Particle-size coefficient of variation

• Streptavidin coating level

• Biotin-binding capacity

• Suspension buffer

• Stabilizer

• Surfactant

• Preservative

• Particle number concentration

• Packaging volume

• Shelf life

• Storage conditions

Binding capacity should be reported using the actual test method and lot-specific results rather than estimated from particle size or solids content.

How Streptavidin–Biotin Binding Works

Streptavidin is a biotin-binding protein with multiple binding sites. When a biotinylated antibody, protein, peptide, or nucleic acid is mixed with SAPS1UM-10, the biotin group interacts with the streptavidin coating on the microsphere surface.

This creates a rapid and stable method for immobilizing a wide range of biomolecules.

A typical structure is:

Microsphere Surface – Streptavidin – Biotin – Capture Molecule

For example:

Microsphere – Streptavidin – Biotinylated Antibody

The immobilized antibody can subsequently capture its corresponding antigen. A fluorescently labeled detection antibody can then be introduced, and the resulting microsphere-associated fluorescence can be measured by flow cytometry.

Advantages of Streptavidin–Biotin Immobilization

• Simple biomolecule attachment

• No direct activation of the microsphere required by the end user

• Compatible with many biotinylated biomolecules

• Flexible capture-molecule orientation

• Suitable for modular assay development

• Convenient replacement of one biotinylated ligand with another

• Compatible with immunoassay and nucleic acid workflows

• Suitable for single-analyte and multiplex research

The final binding performance depends on the biotinylation level, molecular size, incubation conditions, surface accessibility, buffer composition, and steric effects.

Why Choose 1µm Streptavidin-Coated Microspheres?

High Particle Number per Unit Volume

Compared with larger microspheres at the same solids content, 1µm microspheres provide a greater number of individual particles.

This may be useful when an assay requires:

• A large number of independent binding events

• Low microsphere consumption per test

• High-throughput reaction formats

• Small sample volumes

• Microplate-based workflows

• Numerous flow cytometry events

The actual particle number concentration should be determined from the measured particle diameter, particle density, solids content, and lot-specific characterization.

Large Collective Surface Area

A suspension containing many small microspheres can provide substantial total surface area for biomolecule immobilization.

However, usable binding capacity is determined by more than particle surface area. Streptavidin density, coating activity, steric accessibility, ligand size, and biotinylation level also affect the final result.

Modular Assay Design

One streptavidin-coated microsphere platform can be combined with different biotinylated capture molecules.

This allows researchers to develop multiple assays without producing a separate covalent particle conjugate for every antibody, protein, or nucleic acid probe.

Suitable for Fluorescence-Based Detection

SAPS1UM-10 does not need to be intrinsically fluorescent to support a flow cytometry assay.

A fluorescence signal can be introduced through:

• A fluorescently labeled biotinylated ligand

• A fluorescent target molecule

• A fluorescent detection antibody

• A fluorescent secondary antibody

• A fluorescent nucleic acid probe

• A fluorescent reporter conjugate

The reporter fluorescence identifies binding events associated with the microsphere population.

Suitable for Research-to-Production Development

SAPS1UM-10 can support early feasibility testing followed by pilot-scale and bulk manufacturing after technical requirements have been confirmed.

Key Product Features

1µm Nominal Particle Diameter

The micron-scale particle size is suitable for particle-based binding studies and optimized flow cytometry workflows.

Streptavidin-Coated Surface

The particle surface is designed to capture biotinylated biomolecules through streptavidin–biotin affinity.

1% Solids Suspension

The product is supplied at 1% solids and can be diluted according to the required number of microspheres, assay volume, and flow cytometry event rate.

Flexible Biomolecule Immobilization

The same microsphere platform may be used with different biotinylated antibodies, proteins, peptides, or nucleic acid probes.

No End-User Carbodiimide Activation Required

When a suitable biotinylated molecule is available, it can be immobilized without performing EDC/NHS activation of carboxyl groups.

Compatible with Fluorescent Reporter Assays

The microspheres can be combined with fluorescence-based detection reagents for flow cytometry analysis.

Suitable for Singleplex and Multiplex Research

Different microsphere populations, capture ligands, or fluorescence codes may be evaluated during multiplex assay development.

Bulk Manufacturing Capability

Shanghai SanYu Biotechnology Co., Ltd. supports sample evaluation, pilot production, repeated orders, OEM projects, and bulk supply.

Applications in Flow Cytometry

Bead-Based Immunoassay Development

SAPS1UM-10 may be used as a solid-phase support in research assays for detecting:

• Proteins

• Antibodies

• Antigens

• Cytokines

• Hormones

• Biomarkers

• Pathogen-associated targets

• Cell-derived molecules

• Environmental analytes

• Research compounds

A typical bead-based sandwich assay uses:

1. A biotinylated capture antibody immobilized on the microsphere.

2. A target analyte captured from the sample.

3. A fluorescent detection antibody bound to the captured target.

4. Flow cytometry to measure microsphere-associated fluorescence.

Biotinylated Antibody Immobilization

Biotinylated antibodies can be attached to SAPS1UM-10 without direct chemical activation of the microsphere.

Potential research applications include:

• Antigen detection

• Antibody screening

• Hybridoma screening

• Protein interaction analysis

• Assay feasibility testing

• Capture-antibody comparison

• Detection-reagent optimization

Protein and Peptide Binding Studies

Biotinylated proteins and peptides may be immobilized for:

• Antibody-binding studies

• Receptor–ligand interaction studies

• Protein–protein interaction research

• Epitope screening

• Biomarker research

• Drug-discovery assays

Nucleic Acid Capture

Biotinylated oligonucleotides, DNA, RNA, and aptamers may be attached to the microsphere surface.

Potential applications include:

• Hybridization assays

• Mutation-detection research

• Genotyping method development

• Sequence-specific capture

• Nucleic acid binding studies

• Aptamer-based detection

• Molecular diagnostic research

• PCR-product capture

Cell-Surface Binding Research

When coated with a suitable biotinylated ligand, SAPS1UM-10 may be evaluated for binding to specific cell-surface receptors.

The interaction between the particles and cells should be carefully optimized because microsphere concentration, ligand density, incubation time, temperature, cell condition, and washing procedures may affect the result.

Multiplex Flow Cytometry Research

SAPS1UM-10 may be incorporated into a multiplex platform when it can be distinguished from other microsphere populations.

Potential differentiation strategies include:

• Different particle sizes

• Different internal fluorescence codes

• Different fluorescence intensities

• Different visible colors

• Different reporter channels

• Different surface capture molecules

The standard SAPS1UM-10 product should not be assumed to contain an internal fluorescence code unless this is stated in the product specification.

Biotinylated Molecules Compatible with SAPS1UM-10

SAPS1UM-10 may be evaluated with many types of biotinylated molecules.

Biotinylated Antibodies

Applicable formats may include:

• Full-length IgG

• Fab fragments

• F(ab′)₂ fragments

• Recombinant antibodies

• Single-domain antibodies

• Other engineered antibody formats

The number and location of biotin groups may affect antibody orientation and antigen-binding activity.

Biotinylated Proteins

Potential examples include:

• Antigens

• Enzymes

• Receptors

• Cytokines

• Growth factors

• Binding proteins

• Recombinant fusion proteins

Biotinylated Peptides

Biotinylated peptides may support:

• Epitope mapping

• Antibody screening

• Receptor-binding studies

• Enzyme-substrate research

• Drug-discovery screening

Biotinylated Nucleic Acids

Potential formats include:

• Single-stranded DNA

• Double-stranded DNA

• RNA

• PCR products

• Oligonucleotide probes

• Aptamers

• Modified nucleic acid analogues

Biotinylated Small Molecules

Small biotinylated compounds may be attached when the biotin group remains accessible to the streptavidin surface.

A suitable spacer may reduce steric hindrance between the microsphere surface and the target molecule.

Recommended Biotinylated Ligand Immobilization Workflow

The following protocol is a general development starting point. Final conditions should be optimized according to the biotinylated molecule and intended assay.

1. Resuspend the Microspheres

Allow the microsphere suspension to reach the recommended handling temperature.

Mix the vial thoroughly using gentle inversion, rotation, or controlled vortexing until the suspension is homogeneous.

Avoid excessive foaming.

2. Calculate the Required Microsphere Quantity

Determine the amount of SAPS1UM-10 required according to:

• Number of tests

• Microspheres per test

• Assay volume

• Required binding capacity

• Expected sample concentration

• Desired flow cytometry event count

Do not calculate ligand loading from solids content alone. Use lot-specific binding capacity when available.

3. Wash the Microspheres

Transfer the required microsphere suspension to a clean, low-binding tube.

Wash the particles using a compatible binding buffer to remove storage additives that may interfere with the assay.

Possible separation approaches include:

• Centrifugation

• Membrane filtration

• Tangential-flow filtration

• Other validated particle-recovery methods

The appropriate method depends on the microsphere matrix, density, particle size, formulation, and processing volume.

4. Prepare the Biotinylated Ligand

Dilute the biotinylated antibody, protein, peptide, or nucleic acid in a compatible buffer.

The buffer should not contain excessive free biotin or other substances that compete for streptavidin binding sites.

5. Incubate the Microspheres with the Ligand

Combine the washed microspheres with the biotinylated ligand.

Incubate with gentle mixing to keep the particles suspended and promote uniform ligand exposure.

Parameters requiring optimization include:

• Ligand concentration

• Microsphere concentration

• Incubation time

• Temperature

• Buffer composition

• Buffer pH

• Mixing speed

• Total reaction volume

6. Remove Unbound Ligand

Separate and wash the microspheres to remove unbound biotinylated molecules.

Insufficient washing may increase background signal, while excessive or harsh washing may reduce recovery.

7. Block the Microsphere Surface

Use an application-compatible blocking reagent to reduce non-specific adsorption.

Potential blockers may include:

• Bovine serum albumin

• Casein

• Fish gelatin

• Non-immune immunoglobulin

• Synthetic blocking polymers

• Commercial particle-blocking buffers

The blocking agent must be selected according to the assay matrix and detection system.

8. Resuspend and Store the Coated Microspheres

Resuspend the prepared microspheres in a suitable storage or assay buffer.

Evaluate:

• Dispersion stability

• Ligand retention

• Binding activity

• Non-specific binding

• Microbial stability

• Fluorescence background

• Short-term and long-term storage stability

Flow Cytometry Bead Assay Development Workflow

1. Prepare Capture Microspheres

Immobilize the selected biotinylated capture molecule onto SAPS1UM-10.

Wash and block the coated microspheres before testing samples.

2. Prepare Experimental Controls

Recommended controls may include:

• Buffer blank

• Uncoated microsphere control

• Streptavidin microsphere without biotinylated ligand

• Biotinylated ligand-coated microsphere without sample

• Negative sample

• Positive sample

• Reporter-only control

• Isotype control when appropriate

• Single-color controls for multicolor experiments

3. Incubate with the Sample

Combine the prepared microspheres with the test sample.

Optimize:

• Sample volume

• Microsphere concentration

• Incubation time

• Temperature

• Mixing

• Wash conditions

• Sample dilution

• Matrix compatibility

4. Add the Fluorescent Reporter

Add a fluorescent detection antibody, secondary antibody, probe, or reporter molecule.

The reporter fluorophore should be compatible with the lasers and detectors available on the flow cytometer.

5. Wash the Microspheres

Remove unbound fluorescent reporter to reduce background fluorescence.

Maintain adequate microsphere recovery throughout the washing procedure.

6. Resuspend for Flow Cytometry

Resuspend the final microsphere pellet in a clean and compatible acquisition buffer.

Filter the buffer when low particulate background is required.

7. Acquire the Microspheres

Use scatter and fluorescence parameters to identify the microsphere population.

Possible plots include:

• Forward scatter versus side scatter

• Scatter versus reporter fluorescence

• Reporter fluorescence histogram

• Fluorescence area versus fluorescence height

• Scatter area versus scatter width

8. Analyze the Results

Possible analytical outputs include:

• Median fluorescence intensity

• Mean fluorescence intensity

• Percentage of positive microspheres

• Fluorescence distribution width

• Signal-to-background ratio

• Dose-response curve

• Limit-of-detection research

• Repeatability

• Recovery

• Specificity

• Cross-reactivity

The threshold defining a positive microsphere population should be established using appropriate negative controls.

Detection of 1µm Microspheres by Flow Cytometry

The ability to detect 1µm microspheres depends on the flow cytometer.

Important instrument factors include:

• Laser wavelength

• Laser power

• Forward-scatter optics

• Side-scatter optics

• Detector sensitivity

• Optical filter configuration

• Trigger channel

• Trigger threshold

• Flow-cell design

• Sample flow rate

• Electronic noise

• Sheath-fluid cleanliness

Scatter Detection

Some conventional flow cytometers can identify 1µm polymer microspheres using forward and side scatter, while other instruments may require optimized small-particle settings.

The measured scatter signal also depends on the microsphere refractive index and the optical configuration of the instrument.

Fluorescence-Based Detection

When scatter separation is insufficient, fluorescence associated with a bound reporter can provide a more selective method for identifying microsphere events.

Possible triggering strategies include:

• Forward-scatter triggering

• Side-scatter triggering

• Fluorescence triggering

• Combined scatter and fluorescence gating

Start with a Dilution Series

A dilution series helps identify a microsphere concentration that provides:

• Stable event rates

• Minimal coincident events

• Reproducible fluorescence

• Clear background separation

• Suitable acquisition time

• Adequate event counts

Excessively concentrated microspheres may produce swarm detection or coincidence, causing multiple particles to be recorded as one event.

Use Clean Buffers and Tubes

For 1µm particle analysis, particulate contamination from buffers, tubes, pipette tips, and instrument fluidics can interfere with the microsphere population.

Run a buffer blank before acquiring the sample.

Record Instrument Settings

For reproducibility, document:

• Flow cytometer model

• Laser configuration

• Detector filters

• Scatter settings

• Fluorescence settings

• Trigger parameter

• Trigger threshold

• Sample flow rate

• Microsphere dilution

• Acquisition time

• Number of collected events

• Analysis gates

Blocking and Reduction of Non-Specific Binding

Non-specific adsorption can increase background fluorescence and reduce assay sensitivity.

Potential causes include:

• Inadequate blocking

• Excess capture ligand

• Excess fluorescent reporter

• Incompatible buffer

• Hydrophobic sample components

• High protein concentration

• Aggregated antibodies

• Insufficient washing

• Microsphere aggregation

• Sample-matrix interference

Optimize the Blocking Reagent

Compare several blocking formulations rather than assuming one blocker will work for every assay.

Evaluate both:

• Reduction in negative-sample fluorescence

• Retention of positive-sample signal

Optimize Salt and Detergent Levels

Small amounts of compatible detergent may improve suspension stability and reduce non-specific adsorption.

Excessive detergent may affect biomolecule interactions or downstream assay performance.

Titrate the Capture Ligand

Applying more biotinylated ligand than required can increase reagent consumption and may contribute to steric crowding.

Test several ligand concentrations and select the lowest level that maintains suitable assay performance.

Titrate the Reporter

An excessive fluorescent reporter concentration can increase background.

Reporter titration should be performed using positive and negative samples.

Include Appropriate Controls

Controls help distinguish true target binding from:

• Microsphere autofluorescence

• Reporter adsorption

• Streptavidin-related background

• Sample-matrix effects

• Non-specific antibody interactions

Quality Control and Lot-to-Lot Consistency

For flow cytometry reagent development and bulk purchasing, product consistency should be evaluated using agreed specifications.

Potential quality-control parameters include:

• Nominal particle diameter

• Particle-size distribution

• Particle-size coefficient of variation

• Suspension appearance

• Solids content

• Streptavidin coating consistency

• Biotin-binding capacity

• Dispersion performance

• Aggregate level

• Background fluorescence

• Particle recovery

• Microbial control

• Packaging integrity

• Storage stability

Biotin-Binding Capacity

Binding capacity may be measured using a defined biotinylated probe.

The reported value depends on:

• Probe type

• Molecular size

• Number of biotin groups

• Test buffer

• Incubation time

• Detection method

• Calculation basis

The test method should be stated when comparing products or production lots.

Flow Cytometry QC

A flow cytometry-based QC procedure may evaluate:

• Position of the microsphere population

• Reporter fluorescence after binding a standard probe

• Distribution width

• Percentage of positive events

• Background separation

• Repeatability

• Aggregate population

The same instrument and acquisition settings should be used when comparing batches.

Lot-Specific Documentation

Available documentation may include:

• Certificate of Analysis

• Product specification

• Particle-size results

• Solids-content results

• Binding-capacity results

• Safety Data Sheet

• Handling instructions

• Storage recommendations

• Lot number and production date

Required tests and acceptance limits should be agreed upon before bulk production.

Bulk Manufacturing and Customization

Shanghai SanYu Biotechnology Co., Ltd. supports streptavidin microsphere projects from research evaluation to bulk manufacturing.

Research Sample Evaluation

Samples may be used to evaluate:

• Microsphere detection

• Streptavidin activity

• Biotinylated ligand binding

• Particle dispersion

• Non-specific binding

• Flow cytometry compatibility

• Assay feasibility

Pilot-Scale Production

Pilot batches may support:

• Ligand-loading optimization

• Assay development

• Stability testing

• Packaging evaluation

• Quality-control method development

• Customer verification

• Process transfer

Bulk Production

Bulk production can be arranged after the product specifications and quality requirements have been confirmed.

Information required for project evaluation may include:

• Required quantity

• Expected annual demand

• Preferred package size

• Intended application

• Required particle diameter

• Required solids content

• Required binding capacity

• Suspension-buffer requirements

• Preservative restrictions

• Quality-control requirements

• Documentation requirements

• Delivery schedule

• Delivery destination

Custom Particle Sizes

Alternative particle diameters may be discussed for qualified projects.

Particle size affects:

• Particle number

• Collective surface area

• Settling behavior

• Flow cytometry detection

• Washing and recovery

• Assay kinetics

• Biomolecule loading

Custom Streptavidin Loading

Different streptavidin coating levels or binding-capacity specifications may be evaluated according to project requirements and technical feasibility.

Custom Fluorescence Coding

For multiplex flow cytometry projects, internally fluorescent or color-coded streptavidin microspheres may be discussed.

Potential customization directions include:

• Different fluorescence colors

• Different fluorescence intensities

• Multiple internal codes

• Alternative excitation and emission wavelengths

Custom Concentration and Packaging

Possible options may include:

• Alternative solids concentrations

• Laboratory sample sizes

• Pilot-scale packages

• Bulk containers

• Customer-specific fill volumes

• OEM labels

• Customer-specific catalog numbers

Handling and Storage Recommendations

Follow the storage conditions stated on the final product label, technical data sheet, and Certificate of Analysis.

General recommendations include:

• Store refrigerated when specified.

• Do not freeze unless freeze-thaw stability has been validated.

• Keep the container tightly closed.

• Store the vial upright.

• Protect protein-coated microspheres from contamination.

• Mix thoroughly before sampling.

• Avoid allowing the particles to dry.

• Use clean pipette tips and low-binding tubes.

• Do not return diluted material to the original bottle.

• Avoid prolonged storage of prepared working dilutions.

• Record the lot number and opening date.

Resuspension

Microspheres may settle during storage.

Before use:

1. Allow the vial to reach the recommended handling temperature.

2. Gently invert or rotate the vial.

3. Apply controlled vortexing when permitted.

4. Confirm that the suspension is homogeneous.

5. Inspect for visible or irreversible aggregates.

Avoid Freezing

Freezing can cause microsphere aggregation and may reduce streptavidin binding activity.

Frozen product should be evaluated before use and should not be assumed to meet the original specification.

Avoid Free Biotin Contamination

Buffers, blocking reagents, samples, or supplements containing free biotin can occupy streptavidin-binding sites.

Review all assay components when unexpectedly low ligand binding is observed.

Frequently Asked Questions

What is SAPS1UM-10?

SAPS1UM-10 is a 1µm streptavidin-coated microsphere suspension with 1% solids, developed for binding biotinylated molecules in flow cytometry and bead-based assay research.

Who manufactures SAPS1UM-10?

SAPS1UM-10 is supplied by Shanghai SanYu Biotechnology Co., Ltd.

What is the particle diameter?

The nominal particle diameter is 1µm.

The measured diameter and particle-size distribution should be confirmed using the lot-specific documentation.

What is the solids content?

SAPS1UM-10 is supplied at 1% solids.

Are the microspheres magnetic?

SAPS1UM-10 should not be assumed to be magnetic unless magnetic properties are specifically stated in the product specification.

Separation and washing procedures should be selected according to the confirmed particle matrix.

Are the microspheres fluorescent?

The standard SAPS1UM-10 product should not be assumed to contain an internal fluorescent dye unless fluorescence is specifically listed in the specification.

Flow cytometry detection may use scatter or fluorescence from a bound reporter.

What molecules can bind to the microspheres?

The streptavidin surface may be used to capture biotinylated antibodies, proteins, peptides, oligonucleotides, DNA, RNA, aptamers, and other biotin-containing molecules.

Can antibodies be attached directly?

A biotinylated antibody can be attached through the streptavidin–biotin interaction.

An unmodified antibody will not specifically bind through this mechanism unless it is first biotinylated or connected through another compatible reagent.

Is EDC/NHS activation required?

EDC/NHS activation is generally not required when attaching a biotinylated molecule to a streptavidin-coated microsphere.

What is the binding capacity?

Binding capacity should be confirmed using the lot-specific Certificate of Analysis or technical specification.

It should not be calculated from solids content alone.

Can SAPS1UM-10 be used in flow cytometry?

Yes. It may be evaluated in flow cytometry bead assays, provided that the instrument can detect the 1µm particle population or the associated fluorescent reporter signal.

Can a conventional flow cytometer detect 1µm microspheres?

Detection is instrument dependent.

Some conventional flow cytometers can resolve 1µm particles using optimized scatter settings, while others may require fluorescence-triggered detection or a small-particle flow cytometry platform.

Can SAPS1UM-10 be used in a sandwich immunoassay?

Yes. A biotinylated capture antibody can be immobilized on the microsphere, followed by target capture and detection with a fluorescent reporter antibody.

All assay conditions must be optimized experimentally.

Can the product be used for nucleic acid assays?

Yes. Biotinylated oligonucleotides, DNA, RNA, PCR products, and aptamers may be evaluated for immobilization or target capture.

Can SAPS1UM-10 be used in multiplex assays?

It may be incorporated into a multiplex system when the particle population can be distinguished from other microsphere populations.

A customized fluorescent coding strategy may be required.

How should the microspheres be washed?

The washing method depends on the particle matrix, density, buffer, and processing volume.

Centrifugation or membrane-based methods may be evaluated and validated for recovery.

Why is microsphere recovery low after washing?

Possible causes include:

• Inadequate centrifugal force

• Short centrifugation time

• Loss during supernatant removal

• Particle adhesion to tubes

• Membrane retention

• Aggregation

• Incorrect particle-separation method

How can non-specific binding be reduced?

Optimize the blocking agent, wash conditions, detergent level, capture-ligand concentration, reporter concentration, incubation time, and sample dilution.

Can buffers containing free biotin be used?

Free biotin may compete with the biotinylated capture molecule for streptavidin-binding sites and should generally be avoided during ligand immobilization.

Should the product be diluted before use?

Yes. The working concentration should be selected according to the required number of particles, assay volume, event rate, and binding capacity.

How should SAPS1UM-10 be mixed?

Gently invert, rotate, or use controlled vortexing until the suspension is homogeneous.

Avoid excessive foaming and harsh treatment.

Can the product be frozen?

Freezing is generally not recommended because it may cause irreversible aggregation and loss of functional activity.

Is bulk production available?

Yes. Shanghai SanYu Biotechnology Co., Ltd. supports sample evaluation, pilot batches, repeated orders, and bulk manufacturing.

Can particle size or concentration be customized?

Alternative particle sizes, solids concentrations, package sizes, streptavidin coating levels, and fluorescence coding options may be discussed according to technical feasibility.

What information is required for a quotation?

Please provide:

• Product catalog number

• Required quantity

• Expected annual demand

• Preferred package size

• Intended application

• Flow cytometer model

• Required particle number per test

• Type of biotinylated molecule

• Required binding capacity

• Buffer restrictions

• Preservative restrictions

• Quality-control requirements

• Documentation requirements

• Delivery destination

Request a Sample or Bulk Quotation

SAPS1UM-10 1µm Streptavidin Microspheres provide a flexible particle platform for biotinylated biomolecule immobilization, flow cytometry bead assays, immunoassay research, protein-binding studies, nucleic acid capture, and research reagent production.

The product offers:

• 1µm nominal particle diameter

• Streptavidin-coated surface

• 1% solids suspension

• Capture of biotinylated biomolecules

• Compatibility with fluorescence reporter assays

• Sample and pilot-batch supply

• Bulk manufacturing capability

• Custom product-development support

For sample evaluation or bulk purchasing, provide the intended application, required quantity, biotinylated molecule, desired binding capacity, flow cytometer configuration, packaging requirements, and quality-control specifications.

Product Name: 1µm Streptavidin Microspheres
Catalog Number: SAPS1UM-10
Manufacturer: Shanghai SanYu Biotechnology Co., Ltd.
Nominal Particle Diameter: 1µm
Surface Modification: Streptavidin
Solids Content: 1%
Primary Application: Flow Cytometry and Bead-Assay Research
Supply Capability: Samples, Pilot Batches, and Bulk Production
Intended Use: Research Use Only. Not for diagnostic or therapeutic use.